Bioenergetics Core

核心C：生物能量学剖析核心 导演：克雷格·卡诺·比森博士 药物发现与生物医学科学系教授 项目摘要 生物能学分析核心设施的主要目标是支持Cobre调查人员在 与细胞氧化还原和初级能量代谢有关的代谢物通量的特征 主要和次要细胞氧化还原物种的来源。该设施提供访问传统的、 “黄金标准”技术，如同位素、辐射和光谱分析。核心也是一个 用于测量海马生物科学胞外通量(XF)荧光技术的开发现场 使用多孔板实时监测代谢通量(即耗氧量、二氧化碳和乳酸排泄量)。这个 基本的海马XF应用支持高通量新陈代谢测量，样本量小， 改变了代谢通量定量分析的效用。XF的创新改编 在核心设施中开发的技术正在提供对氧化还原的实时通量测量的访问 细胞和组织中的物种，更重要的是，通过使用 药物或遗传干预。我们已经协调了最近的这些战略技术 将收购转化为核心，为Cobre调查人员和他们的努力提供分析支持 协作者，同时扩展技术以适应新的工作，并进一步支持测量 提高了翻译潜力。在科布雷第一阶段，核心被称为“代谢组学核心”。 这提供了除了传统的单时间点量化之外的新生海马XF技术 通过LC-MS或核磁共振技术测定代谢物浓度。如下所述，这些传统的代谢组学 对于Cobre的首席调查人员来说，技术不如XF技术有用，特别是 后者技术先进，传统代谢组学已经成熟。因为传统的代谢组学 分析现在可以商业化，从而降低了成本(很像学术DNA低聚物或 多肽核心已过时)，代谢组学核心已被重新命名为“生物能量谱” 核心“以反映我们开发的最好地支持我们的调查人员的XF服务，对于这些服务，有 没有可供选择的外部资源。事实上，由于XF技术使用的是活样本，它们不太可能做到这一点 很容易被“外包”。

CORE C: Bioenergetics Profiling Core Director: Craig Cano Beeson, Ph.D. Professor, Department of Drug Discovery and Biomedical Sciences Project Summary The primary goal of the Bioenergetics Profiling core facility is to support COBRE investigators in the characterization of metabolite fluxes related to cellular redox and primary energy metabolism – the prevalent sources of both the primary and secondary cellular redox species. The facility provides access to traditional, `gold standard' techniques such as isotopomer, radiometric, and spectroscopic analyses. The core is also a development site for the Seahorse Biosciences extracellular flux (XF) fluorometric technology used to measure metabolic fluxes (i.e., oxygen consumption, CO2 and lactate extrusion) in real time using multiwell plates. The basic Seahorse XF applications enable high throughput metabolic measurements with small sample sizes that have transformed the utility of quantitative analyses of metabolic fluxes. Innovative adaptations of the XF technologies developed in the core facility are providing access to real time flux measurements of redox species in cells and tissues and, more importantly, the interrogation of bioenergetics pathways via use of pharmacological or genetic interventions. We have coordinated these recent strategic technological acquisitions into a core that provides analytical support for the efforts of the COBRE investigators and their collaborators while also extending the technology to suit new efforts and further enable measurements with improved translational potential. In the COBRE Phase I stage the core was known as the “Metabolomics Core” that offered nascent Seahorse XF technology in addition to traditional single time-point quantification of metabolite concentrations via LC-MS or NMR techniques. As described below, these traditional metabolomics techniques were not as useful to the lead COBRE investigators as were the XF technologies, particularly as the latter technology advanced and traditional metabolomics has matured. Because traditional metabolomics analyses are now commercially available leading to cost reductions (much as academic DNA oligomer or peptide cores became obsolete), the metabolomics core has been renamed as the “Bioenergetics Profiling Core” to reflect the XF services we have developed that best support our investigators, and for which there are no alternative external resources. Indeed, because XF technologies utilize living samples, it is unlikely they will be easily `out-sourced'.