Bioenergetics Core

核心C：生物能量学分析核心 导演：克雷格卡诺·比森博士 药物发现和生物医学科学系教授 项目摘要 生物能量学分析核心设施的主要目标是支持COBRE研究人员在 与细胞氧化还原和初级能量代谢有关的代谢物通量的表征- 主要和次要细胞氧化还原物质的来源。该设施提供传统的， "黄金标准"技术，如同位素、放射性和光谱分析。核心也是一个 Seahorse Biosciences细胞外通量（XF）荧光技术的开发地点，用于测量 代谢通量（即，氧消耗、CO2和乳酸盐挤出）。的 基本的Seahorse XF应用程序可以实现小样本量的高通量代谢测量， 已经改变了代谢通量定量分析的效用。XF的创新调整 在核心设施中开发的技术正在提供氧化还原的真实的时间通量测量 物种的细胞和组织，更重要的是，通过使用生物能量学途径的审讯， 药物或遗传干预。我们已经协调了这些最近的战略技术 收购到一个核心，为COBRE调查人员的工作提供分析支持， 合作者，同时也扩展技术，以适应新的努力，并进一步使测量与 提高翻译能力。在COBRE第一阶段，核心被称为“代谢组学核心”。 除了传统的单时间点定量外，还提供了新生的Seahorse XF技术。 通过LC-MS或NMR技术测定代谢物浓度。如下文所述，这些传统的代谢组学 对于COBRE的主要研究人员来说，这些技术并不像XF技术那样有用，特别是 后者技术先进，传统的代谢组学已经成熟。因为传统的代谢组学 分析现在是商业上可获得的，导致成本降低（与学术DNA寡聚体或 肽核心已被淘汰），代谢组学核心已更名为"生物能量学分析 核心"，以反映我们开发的XF服务，最好地支持我们的研究人员， 没有其他外部资源。事实上，由于XF技术利用活样本， 很容易被“外包”。

CORE C: Bioenergetics Profiling Core Director: Craig Cano Beeson, Ph.D. Professor, Department of Drug Discovery and Biomedical Sciences Project Summary The primary goal of the Bioenergetics Profiling core facility is to support COBRE investigators in the characterization of metabolite fluxes related to cellular redox and primary energy metabolism – the prevalent sources of both the primary and secondary cellular redox species. The facility provides access to traditional, `gold standard' techniques such as isotopomer, radiometric, and spectroscopic analyses. The core is also a development site for the Seahorse Biosciences extracellular flux (XF) fluorometric technology used to measure metabolic fluxes (i.e., oxygen consumption, CO2 and lactate extrusion) in real time using multiwell plates. The basic Seahorse XF applications enable high throughput metabolic measurements with small sample sizes that have transformed the utility of quantitative analyses of metabolic fluxes. Innovative adaptations of the XF technologies developed in the core facility are providing access to real time flux measurements of redox species in cells and tissues and, more importantly, the interrogation of bioenergetics pathways via use of pharmacological or genetic interventions. We have coordinated these recent strategic technological acquisitions into a core that provides analytical support for the efforts of the COBRE investigators and their collaborators while also extending the technology to suit new efforts and further enable measurements with improved translational potential. In the COBRE Phase I stage the core was known as the “Metabolomics Core” that offered nascent Seahorse XF technology in addition to traditional single time-point quantification of metabolite concentrations via LC-MS or NMR techniques. As described below, these traditional metabolomics techniques were not as useful to the lead COBRE investigators as were the XF technologies, particularly as the latter technology advanced and traditional metabolomics has matured. Because traditional metabolomics analyses are now commercially available leading to cost reductions (much as academic DNA oligomer or peptide cores became obsolete), the metabolomics core has been renamed as the “Bioenergetics Profiling Core” to reflect the XF services we have developed that best support our investigators, and for which there are no alternative external resources. Indeed, because XF technologies utilize living samples, it is unlikely they will be easily `out-sourced'.