FAIMS-Selected Reaction Monitoring to Quantitative Protein Networks in Disease

描述(申请人提供)：强大的研究技术的进步，如质谱学，使研究人员不仅能够对生物体或细胞器系统的蛋白质互补的许多变体进行分类，而且最近还使用定量蛋白质组学工具，在空间和时间上专门对细胞内的每种蛋白质成分进行定量。对FAIMS增强型TSQ-Vantage三重四极杆的收购对于ISB的研究基础设施至关重要，用于开发和广泛传播下一代技术和仪器给从博士生和博士后到ISB和其他合作研究所有经验的PI的研究人员。由实验学家和计算生物学家使用，通过拟议的仪器收集的数据将通过识别和定量多肽及其翻译后修饰来增强对蛋白质功能和作用的理解，并最终将向研究人员提供与生物体的形式和功能有关的蛋白质组动力学。收购FAIMS增强型TSQ-Vantage三重四极杆质谱仪将大大提高我们在许多系统中对蛋白质进行专门定量的能力，这将使ISB的研究人员能够推动这项技术在识别对先天性免疫反应、过氧化物酶功能和DNA修复至关重要的蛋白质数量变化方面提供更深入的见解。这将通过筛选出化学背景噪音，特别是目标蛋白质的蛋白质组特征，并以非常高的特异性和灵敏度对它们进行定量，这是目前其他质谱仪无法实现的。最后，这种仪器将使研究人员能够组装出特定系统的蛋白质补充的完整定量图谱，并从基因及其转录本的相互作用网络中做出推断。获得这项质谱学技术将有助于发展和完成目前在ISB及其合作伙伴正在进行的生物学研究。

DESCRIPTION (provided by applicant): Advances in powerful research technologies such as mass spectrometry have enabled researchers to not only catalogue the many variants of the protein complement of an organism or organelle system, but more recently, using quantitative proteomic tools, to specifically quantitate each protein component both spatially and temporally within the cell. The acquisition of the FAIMS-enhanced TSQ-Vantage triple-quadrupole is crucial to the research infrastructure at ISB for development and broad dissemination of next-generation technology and instrumentation to researchers ranging from Ph.D. students and post-docs to experienced PI's at ISB and other collaborating institutes. Used by experimentalists and computational biologists, the data collected with the proposed instrumentation will enhance the understanding of protein function and action through identification and quantitation of peptides, their posttranslational modifications and will ultimately inform researchers of proteome dynamics that are responsible for an organism's form and functions. The acquisition of a FAIMS-enhanced TSQ-Vantage triple-quadrupole mass spectrometer will substantially improve our ability to specifically quantitate proteins across many systems, which will enable ISB researchers to drive the technology to provide greater insights in identifying protein quantitative changes important to innate immune response, peroxisome function and DNA repair. This will be achieved through the ability to screen out chemical background noise, specifically target proteotypic signatures of target proteins and quantitate them with very high specificity and sensitivity not currently achievable with other mass spectrometers. Finally, this instrument will enable researchers to assemble a complete quantitative map of the protein complement of a given system and make inferences from the interacting network of genes and their transcripts. The acquisition of this mass spectrometry technology will aid in the development and completion of biological studies currently underway at ISB as well as with its collaborating partners.