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Proteomic Core

蛋白质组核心

基本信息

批准号：
8539500
负责人：
Robert L Moritz
金额：
$ 44.34万
依托单位：
INSTITUTE FOR SYSTEMS BIOLOGY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8539500
关键词：
Address Adopted Affinity Affinity Chromatography Algorithms Amines Amino Acids Architecture Benchmarking Binding Binding Proteins Biological Assay Biotin Cell Extracts Cells Chemicals Chromatin Chromosomes Cleaved cell Collaborations Communities Complex Complex Mixtures Computer software Coupling Cross-Linking Reagents Crosslinker DNA DNA-Protein Interaction Data Data Analyses Data Set Databases Detection Development Dissociation Effectiveness Enzymes Esters Evaluation Experimental Designs Fractionation Future Gases Gene Expression Genes Genetic Transcription Goals Homo Hydrolysis Informatics Ions Kinetochores Label Length Lipids Macromolecular Complexes Manuals Manufacturer Name Manuscripts Maps Mass Spectrum Analysis Measurement Measures Methodology Metric Modification Molecular Profiling Nucleic Acid Probes Nucleic Acids Peptides Phase Post-Translational Protein Processing Preparation Proteins Proteome Proteomics Publishing RNA Polymerase II Reagent Regulatory Element Relative (related person)Research Research Personnel Resources Roentgen Rays Role SAGA Sampling Signal Transduction Spectrometry Structure Subcategory System Systems Biology Techniques Technology Testing Training Training and Education Validation Work Yeasts analytical tool base cell growth regulation computerized tools crosslink data acquisition data exchange deep sequencing design histone acetyltransferase improved in vivo instrument interest ion mobility locked nucleic acid metabolomics new technology novel novel strategies open source operation peroxisome preference promoter protein aminoacid sequence protein complex protein protein interaction research study software development success technology development tool transcription factor

项目摘要

Overview. The proteomics core provides cutting-edge proteomics capabilities to the Center, ISB and many collaborators locally and worldwide. Recent accomplishments include: development of analytical and computational tools enabling comprehensive and systematic analysis of proteomes, subproteomes, and post translational modifications; development of software suites for evaluation and validation of proteomic datasets; and the design and implementation of targeted quantitative mass spectrometry (MS) experiments to analyze proteomes and subcellular proteomes. The core equipped with state-of-the-art MS technologies (see Resources) and will continue to disseminate and promote tools for high quality quantitative data acquisition and rapid implementation of new technologies. The core provides training and assistance on MS operation and experimental design, and assists with the Proteomics Informatics course (See Education and Training). Because the core is so integral to Center research, we provide below a description of ongoing technology development. Quantitative Proteomics. The core is a world leader in the development and application of both label and label-free quantitative proteomics for expression profiling and analysis of macromolecular assemblages. For example, the core, in collaboration with the Aitchison group, developed a novel automated approach to quantify peptides in SILAC experiments (QTIPs) along with new approaches for isolation of macromolecular complexes. These approaches significantly improved the identification of in vivo relevant interactions and led to extensive definition of signaling networks involved in peroxisome induction

概况.蛋白质组学核心为中心，ISB和当地和世界各地的许多合作者提供尖端的蛋白质组学能力。最近的成就包括：开发分析和计算工具，实现蛋白质组，亚蛋白质组和翻译后修饰的全面和系统的分析;开发软件套件，用于蛋白质组数据集的评估和验证;以及设计和实施靶向定量质谱（MS）实验，以分析蛋白质组和亚细胞蛋白质组。该核心配备了最先进的MS技术（请参阅参考资料），并将继续传播和推广用于高质量定量数据采集和快速实施新技术的工具。核心提供MS操作和实验设计的培训和帮助，并协助蛋白质组学信息学课程（见教育和培训）。由于核心是中心研究的组成部分，我们在下面提供了正在进行的技术开发的描述。定量蛋白质组学。核心是一个世界领先的开发和应用的标签和无标签的定量蛋白质组学的表达谱和分析的大分子组装。例如，核心，与艾奇逊集团合作，开发了一种新的自动化方法来量化SILAC实验（QTIPs）中的肽，沿着分离大分子复合物的新方法。这些方法显著改善了体内相关相互作用的鉴定并导致广泛的定义信号网络参与过氧化物酶体诱导