MicroXCT-200 X-ray Scanner

MicroXCT-200 X射线扫描仪

• 批准号: 8049942
• 负责人: BJORN REINO OLSEN
• 金额: $ 58.8万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8049942
• 关键词: 3-Dimensional; Address; Affect; Aging; Biological; Blood Vessels; Bone Regeneration; Data; Degenerative polyarthritis; Dental Schools; Diagnostic radiologic examination; Disease; Disease Progression; Electron Microscopy; Eye diseases; Funding; Gene Mutation; Goals; High Resolution Computed Tomography; Histology; Human; Imaging technology; Inborn Genetic Diseases; Inflammatory; Joints; Marketing; Medicine; Microscope; Mus; Mutation; Natural regeneration; Osteoporosis; Process; Research; Research Personnel; Research Project Grants; Resolution; Roentgen Rays; Sampling; Skeleton; System; Techniques; Tooth structure; Universities; bone; bone loss; detector; eye blood vessel; human disease; innovation; instrument; medical schools; mouse model; novel; repaired; soft tissue; submicron; tomography; ultra high resolution

DESCRIPTION (provided by applicant): In this application, a group of Harvard University investigators seeks funding to purchase an ultra high resolution computed tomography system for 3-dimensional structural analyses of developing, growing and aging bones, joints, teeth, eyes and blood vessels of genetically modified mice. The goal is to stimulate funded research projects on disease mechanisms of inherited disorders of the skeleton, osteoporosis, osteoarthritis, inflammatory bone loss, degenerative eye diseases and vascular anomalies and accelerate progress in studies of bone repair and tooth regeneration. Existing and new mouse models of disease will be used to identify critical mechanistic steps in disease progression, identify novel targets for therapy and strategies for repair/regeneration. Many of the genetic alterations in the mice being studied are identical to gene mutations responsible for inherited disorders in humans, while others are affecting processes that are altered in human disease. The data resulting from this research are therefore highly relevant to understanding mechanisms of human disease. The proposed high resolution Xradia MicroXCT-200 scanner, currently unavailable at Harvard Medical School/Harvard School of Dental Medicine, will enable investigators to address critical questions and collect data that are beyond the limits of current imaging technology. The instrument incorporates several innovative technical features that enable it to outperform all other 3-dimensional X-ray microscopes currently on the market for micro-tomography of biological samples. With proprietary PhaseEnhanced detectors, the MicroXCT makes high resolution (submicron) 3-dimensional histology of both mineralized and soft tissues possible. By combining innovative use of contrast-enhancing techniques of electron microscopy with ultrahigh resolution microCT the investigators believe data can be acquired even in cases where X-ray imaging is otherwise impossible. The acquisition of the instrument will accelerate outstanding research into disease- associated processes in mouse models of human diseases. PUBLIC HEALTH RELEVANCE: This project proposes the use of a new high resolution computed tomography instrument, Xradia MicroXCT-200, to obtain three-dimensional images of normal and diseased bones, cartilage, eye tissues and blood vessels in mouse models of human diseases. The instrument has capabilities that go beyond those of existing microCT scanners and will enable investigators to collect novel data on the progression and consequences of disease-causing processes in both mineralized and soft tissues. This new technology will accelerate progress in a number of existing research projects and generate new insights related to major diseases, such as genetic disorders of bones and joints, osteoporosis, arthritis, vascular anomalies, macular degeneration, periodontal disease and tumors.

描述(申请人提供)：在这份申请中，哈佛大学的一组研究人员寻求资金购买超高分辨率计算机断层扫描系统，用于对转基因小鼠发育、生长和老化的骨骼、关节、牙齿、眼睛和血管进行三维结构分析。其目标是促进关于骨骼遗传性疾病、骨质疏松症、骨关节炎、炎症性骨丢失、退行性眼病和血管异常的疾病机制的资助研究项目，并加快骨修复和牙齿再生的研究进展。现有的和新的小鼠疾病模型将用于确定疾病进展的关键机制步骤，确定新的治疗目标和修复/再生策略。在被研究的小鼠中，许多基因变化与导致人类遗传性疾病的基因突变相同，而其他一些基因变化正在影响人类疾病的变化过程。因此，这项研究得出的数据与理解人类疾病的机制高度相关。拟议的高分辨率XRadia MicroXCT-200扫描仪目前尚未在哈佛医学院/哈佛大学牙医学院获得，它将使研究人员能够解决关键问题并收集超出当前成像技术限制的数据。该仪器结合了几个创新的技术特征，使其性能优于目前市场上所有其他生物样品微断层扫描的三维X射线显微镜。利用专有的PhaseEnhanced探测器，MicroXCT使矿化组织和软组织的高分辨率(亚微米)三维组织学成为可能。通过将电子显微镜的对比度增强技术与超高分辨率显微CT相结合，研究人员相信，即使在X射线成像不可能的情况下，也可以获得数据。该仪器的收购将加速对人类疾病小鼠模型中与疾病相关的过程的杰出研究。 与公共卫生相关：该项目建议使用一种新的高分辨率计算机断层成像仪器XRadia MicroXCT-200，以获得人类疾病小鼠模型中正常和病变的骨骼、软骨、眼组织和血管的三维图像。该仪器具有超越现有MicroCT扫描仪的能力，将使研究人员能够收集关于矿化组织和软组织中致病过程的进展和后果的新数据。这项新技术将加快一些现有研究项目的进展，并产生与重大疾病相关的新见解，如骨骼和关节遗传性疾病、骨质疏松症、关节炎、血管异常、黄斑变性、牙周病和肿瘤。