A novel ion source for efficient coupling of liquid chromatography and mass spect

用于高效耦合液相色谱和质谱的新型离子源

• 批准号: 8057752
• 负责人: VADYM D BERKOUT
• 金额: $ 20万
• 依托单位: MASSTECH, INC.
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8057752
• 关键词: Affinity; Automation; Biological; Biological Process; Biological Sciences; Buffers; Cells; Charge; Chromatography; Complex; Complex Mixtures; Coupled; Coupling; Development; Electrospray Ionization; High Pressure Liquid Chromatography; Hydrophobic Interactions; Ion Exchange; Ions; Liquid Chromatography; Liquid substance; Mass Spectrum Analysis; Molecular Sieve Chromatography; Peptides; Phase; Play; Procedures; Protein Analysis; Proteins; Proteomics; Reagent; Research; Resolution; Role; Sampling; Solutions; Source; Speed; System; Techniques; Tissues; Trifluoroacetic Acid; clinical application; drug discovery; high throughput technology; innovation; interest; ion source; ionization; ionization technique; novel; success

DESCRIPTION (provided by applicant): The primary objective of this proposal is to develop a novel ion source for the efficient on-line coupling of a wide array of chromatographic techniques with mass spectrometry. The use of liquid chromatography separation is a prerequisite for high-throughput mass spectrometry analysis of complex proteomics samples. Commonly used electrospray ionization (ESI), although allowing a direct coupling, imposes significant limitations on the mobile phases and additives used in LC separations. The most widely used separation technique, reversed-phase liquid chromatography, is performed in non-optimal conditions to satisfy operational requirements of the electrospray ion source. The proposed ion source will overcome these limitations and provide efficient analyte ionization from a wide range of liquid sample compositions. Thus, the ion source will allow RP-LC to perform in optimal conditions and also the use of chromatographic techniques not previously amenable by direct ESI-MS analysis. PUBLIC HEALTH RELEVANCE: Proteomics studies biological processes by the systematic analysis of proteins expressed in a cell or tissue. It plays an important role in modern life sciences, drug discovery, and clinical applications. We propose a new high-throughput technology for the efficient on- line coupling of a wide array of chromatographic separation techniques with mass spectrometry.

描述（由申请人提供）：本提案的主要目的是开发一种新型离子源，用于多种色谱技术与质谱法的有效在线耦合。液相色谱分离的使用是复杂蛋白质组学样品高通量质谱分析的先决条件。通常使用的电喷雾电离（ESI）虽然允许直接偶联，但对LC分离中使用的移动的相和添加剂施加了显著的限制。最广泛使用的分离技术，反相液相色谱，在非最佳条件下进行，以满足电喷雾离子源的操作要求。所提出的离子源将克服这些局限性，并提供有效的分析物电离范围广泛的液体样品组合物。因此，离子源将允许RP-LC在最佳条件下进行，并且还使用先前不适合直接ESI-MS分析的色谱技术。 公共卫生相关性：蛋白质组学通过系统分析细胞或组织中表达的蛋白质来研究生物过程。它在现代生命科学、药物发现和临床应用中发挥着重要作用。我们提出了一种新的高通量技术，用于高效的在线耦合的广泛阵列的色谱分离技术与质谱。