A Novel Method of Ion Fragmentation in Tandem Mass Spectrometry

串联质谱中离子断裂的新方法

• 批准号: 7414572
• 负责人: VADYM D BERKOUT
• 金额: $ 40.92万
• 依托单位: SCIENCE AND ENGINEERING SERVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2010-01-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7414572
• 关键词: Acceleration; Address; Area; Biological; Biological Process; Biological Sciences; C-Peptide; Cell physiology; Cells; Classification; Cleaved cell; Dependence; Development; Digestion; Dissociation; Electron Transport; Electronics; Gases; Goals; Ions; Mass Spectrum Analysis; Methods; Object Attachment; Peptides; Performance; Play; Post-Translational Protein Processing; Protein Analysis; Proteins; Proteome; Proteomics; Research; Role; Sampling; Source; Speed; Structure; Techniques; Time; Tissues; Vertebral column; base; clinical application; drug discovery; high throughput technology; human DNA sequencing; instrument; mass spectrometer; novel; small molecule; tandem mass spectrometry

DESCRIPTION (provided by applicant): The primary objective of the proposed research is the development of a tandem orthogonal acceleration time-of-flight (TOF) mass spectrometer for high throughput proteomics applications with enhanced MS/MS capabilities. This will be achieved by building a dual tandem instrument capable of operating in two different MS/MS modes. Alongside with traditional collision induced dissociation (CID), this instrument will feature a novel fragmentation technique based on the interaction of peptide ions with a flow of metastable atoms or molecules. This new fragmentation technique will cleave different peptide backbone bonds than CID with less dependence on peptide composition and preserve labile posttranslational modifications. Incorporating both techniques in one high performance Q-TOF mass spectrometer will allow using these instruments for high throughput proteomics applications. Proteomics studies biological processes by the systematic analysis of the proteins expressed in a cell or tissue. It plays an important role in modern life sciences, drug discovery, and clinical applications. We propose a new high throughput technology for increasing the efficiency of protein identification and structure elucidation using tandem mass spectrometry.

描述（由申请人提供）：拟议研究的主要目标是开发串联正交加速飞行时间（TOF）质谱仪，用于具有增强MS/MS功能的高通量蛋白质组学应用。这将通过构建能够在两种不同MS/MS模式下操作的双串联仪器来实现。除了传统的碰撞诱导解离（CID）之外，该仪器还将采用一种基于肽离子与亚稳态原子或分子流相互作用的新型碎片化技术。这种新的片段化技术将切割与CID不同的肽骨架键，对肽组成的依赖性较小，并保留不稳定的翻译后修饰。在一个高性能Q-TOF质谱仪中使用这两种技术将允许将这些仪器用于高通量蛋白质组学应用。蛋白质组学通过系统分析细胞或组织中表达的蛋白质来研究生物过程。它在现代生命科学、药物发现和临床应用中发挥着重要作用。我们提出了一种新的高通量技术，提高效率的蛋白质鉴定和结构阐明使用串联质谱。