A novel fragmentation technique for tandem mass spectrometry

一种用于串联质谱分析的新型裂解技术

• 批准号: 7996740
• 负责人: VADYM D BERKOUT
• 金额: $ 21.85万
• 依托单位: MASSTECH, INC.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-02 至 2013-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7996740
• 关键词: Biological; Biological Process; Biological Sciences; Cations; Cells; Complex Mixtures; Development; Digestion; Dissociation; Ensure; Goals; Ions; Liquid substance; Mass Spectrum Analysis; Pattern; Peptides; Performance; Play; Post-Translational Protein Processing; Principal Investigator; Procedures; Protein Analysis; Proteins; Proteomics; Research; Resolution; Role; Source; Speed; Structure; Techniques; Tissues; Vertebral column; base; clinical application; design; drug discovery; high throughput technology; innovation; instrument; mass spectrometer; novel; public health relevance; success; tandem mass spectrometry

DESCRIPTION (provided by applicant): The primary goal of this proposal is to develop a novel fragmentation technique based on interaction of peptide ions with radical beam. We plan to demonstrate that this technique will produce different type of fragment ions compared to those obtained in collision-induced dissociation. It will also overcome problems associated with collision-induced dissociation, e.g. preferential fragmentation and facile losses of labile groups involved in many important posttranslational modifications. Combining both fragmentation techniques in one high performance QqTOF mass spectrometer will substantially increase the value of these instruments for high throughput proteomics applications. PUBLIC HEALTH RELEVANCE: Proteomics studies biological processes by the systematic analysis of the proteins expressed in a cell or tissue. It plays an important role in modern life sciences, drug discovery, and clinical applications. We propose a new high throughput technology for increasing the efficiency of protein identification and structure elucidation using tandem mass spectrometry.

描述（由申请人提供）：该提案的主要目标是开发一种基于肽离子与自由基束相互作用的新型碎裂技术。我们计划证明，与碰撞诱导解离中获得的碎片离子相比，该技术将产生不同类型的碎片离子。它还将克服与碰撞引起的解离相关的问题，例如参与许多重要翻译后修饰的不稳定基团的优先断裂和容易丢失。将两种裂解技术结合在一台高性能 QqTOF 质谱仪中将大大增加这些仪器在高通量蛋白质组学应用中的价值。 公共健康相关性：蛋白质组学通过对细胞或组织中表达的蛋白质进行系统分析来研究生物过程。它在现代生命科学、药物发现和临床应用中发挥着重要作用。我们提出了一种新的高通量技术，用于提高使用串联质谱法进行蛋白质鉴定和结构阐明的效率。