ZONA PELLUCIDA BINDING PROTEINS IN SPERM-EGG INTERACTIONS
精子-卵子相互作用中的透明带结合蛋白
基本信息
- 批准号:8102880
- 负责人:
- 金额:$ 18.6万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至 2012-09-30
- 项目状态:已结题
- 来源:
- 关键词:Acrosome ReactionAddressAmino Acid SequenceBindingBinding ProteinsCalcium BindingCandidate Disease GeneCell membraneChickensComplexCysteineCytoplasmDNADatabasesDefectEgg ProteinsExpressed Sequence TagsFertilityFertilizationFutureGene FamilyGene StructureGenesGenomeGerm CellsHumanIn VitroInfertilityKnockout MiceMale InfertilityMusMutationNamesOocytesPH-30 fusion proteinPatternPeptide Sequence DeterminationPeptidyl-Dipeptidase APerivitelline SpacePlayProcessProteinsReproductionRoleSilicon DioxideSperm PenetrationSperm-Ovum InteractionsTestisVertebratesZP1ZP2Zona Pellucidacalmegincontraceptive targetcyritestinegghuman PHEMX proteinhuman malein vivomenparalogous genesperm cellsperm proteinzona pellucida glycoproteinzygote
项目摘要
Project #3: Zona Pellucida Binding Proteins In Sperm Egg Interactions
Reproduction is a complex and multifactorial process, and hundreds of mammalian genes have already been
identified to function in reproduction. The binding of spermatozoa to the cumulus oophorus, the egg zona
pellucida, and eventually the egg plasma membrane are essential steps for the future fusion of gamete
genomes and the initiation of the zygote organization. As might be expected, both sperm and egg proteins
play active roles in the initial and subsequent interactions of sperm and eggs. Many of the proteins that play
key or supporting roles from binding of sperm to the zona pellucida until entry of the sperm DNA into the egg
cytoplasm are largely unknown. Our overlying hypothesis is that the two zona pellucida binding proteins are
critical mammalian sperm proteins in one or more of these steps in fertilization. The Specific Aims are: 1)
Study ZPBP1:ZPBP2 binding and sperm-egg interactions in vitro; 2) Generate and analyze mice lacking
ZPBP1, ZPBP2, or both; and 3) Determine whether men with defective binding of their sperm to eggs have
mutations in ZPBP1 or ZPBP2 genes or additional candidate genes. These studies will be important for
understanding the key proteins and mechanisms involved in sperm:egg interactions in vivo and will help to
define essential proteins implicated in human male infertility.
项目3:精子卵相互作用中的透明带结合蛋白
繁殖是一个复杂和多因素的过程,数百个哺乳动物基因已经
被识别为在繁殖中起作用的。精子与卵丘卵带的结合
透明质膜和最终的卵质膜是未来配子融合的必要步骤
基因组和合子组织的启动。不出所料,精子和卵子蛋白
在精子和卵子最初和随后的相互作用中发挥积极作用。许多起作用的蛋白质
精子与透明带结合直到精子DNA进入卵子的关键或辅助作用
细胞质很大程度上是未知的。我们的假设是两个透明带结合蛋白是
哺乳动物精子在受精过程中的一个或多个步骤中的关键蛋白质。具体目标是:1)
ZPBP1:ZPBP2结合及体外精子-卵子相互作用的研究2)缺乏小鼠的建立和分析
ZPBP1、ZPBP2或两者兼而有之;以及3)确定精子与卵子结合有缺陷的男性是否有
ZPBP1或ZPBP2基因突变或其他候选基因。这些研究将对以下方面具有重要意义
了解精子中的关键蛋白质和机制:卵子在体内的相互作用,将有助于
定义与人类男性不育有关的基本蛋白质。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MARTIN M. MATZUK其他文献
MARTIN M. MATZUK的其他文献
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{{ truncateString('MARTIN M. MATZUK', 18)}}的其他基金
Disruption of semen liquefaction using specific KLK3 inhibitors as a new contraceptive
使用特定 KLK3 抑制剂作为新避孕药破坏精液液化
- 批准号:
10682061 - 财政年份:2022
- 资助金额:
$ 18.6万 - 项目类别:
Kinases as Therapeutic Targets for Endometriosis
激酶作为子宫内膜异位症的治疗靶点
- 批准号:
10674987 - 财政年份:2022
- 资助金额:
$ 18.6万 - 项目类别:
Disruption of semen liquefaction using specific KLK3 inhibitors as a new contraceptive
使用特定 KLK3 抑制剂作为新避孕药破坏精液液化
- 批准号:
10764639 - 财政年份:2022
- 资助金额:
$ 18.6万 - 项目类别:
Disruption of semen liquefaction using specific KLK3 inhibitors as a new contraceptive
使用特定 KLK3 抑制剂作为新避孕药破坏精液液化
- 批准号:
10419647 - 财政年份:2022
- 资助金额:
$ 18.6万 - 项目类别:
Disruption of semen liquefaction using specific KLK3 inhibitors as a new contraceptive
使用特定 KLK3 抑制剂作为新避孕药破坏精液液化
- 批准号:
10598585 - 财政年份:2022
- 资助金额:
$ 18.6万 - 项目类别:
Kinases as Therapeutic Targets for Endometriosis
激酶作为子宫内膜异位症的治疗靶点
- 批准号:
10532966 - 财政年份:2022
- 资助金额:
$ 18.6万 - 项目类别:
Targeting testis-specific ubiquitin-proteasome pathways for male contraception
针对男性避孕的睾丸特异性泛素蛋白酶体途径
- 批准号:
10018522 - 财政年份:2019
- 资助金额:
$ 18.6万 - 项目类别:
Functional genomics and DEC-Tec to identify germ cell-specific contraceptives
功能基因组学和 DEC-Tec 鉴定生殖细胞特异性避孕药
- 批准号:
10164823 - 财政年份:2017
- 资助金额:
$ 18.6万 - 项目类别:
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