Correlative and Advanced Electron Microscopy

项目总监/首席调查员(最后、第一、中间)：高盛，耶鲁大学E.(Svitina-Core C) 项目总结(见说明)： 随着生物学的基础研究越来越关注发生在大分子水平上的事件， 在分子和超分子水平上的结构研究变得特别重要。电子显微镜 提供高分辨率的结构信息，其分辨率甚至超过了应用范围 最先进的光学显微镜方法。电子显微镜的主要局限性是它的适用性 只有到了“死”样本，才能通过关联固定的结构组织来部分克服 在固定前使用光学显微镜方法记录其动态行为的样品。这个电子 显微镜核心单元将为该计划提供包括样品制备在内的电子显微镜服务 并用多种电子显微镜技术进行分析。核心还将发展先进的 特别适用于分子研究的相关光学和电子显微镜技术 马达和囊泡在细胞和无细胞运动系统中的运输。这些新技术也将成为 向核心用户提供。 相关性(请参阅说明)： 电子显微镜是一种独特的工具，可以深入了解组织、细胞和 分子。这样的结构信息为理解复杂的生物过程铺平了道路， 没有它，就不可能与疾病作斗争。 项目/

Program Director/Principal Investigator (Last, First, Middle): Goldman, Yale E. (Svitkina-Core C) PROJECT SUMMARY (See instructions): As fundamental research in biology increasingly focuses on events occurring at the macromolecular level, structural studies at molecular and supramolecular levels become especially important. Electron microscopy provides high resolution structural information, which exceeds in resolution and a span of applications even most advanced light microscopic approaches. The major limitation of electron microscopy, its applicability only to "dead" samples, can be partially overcome by correlating the structural organization of a fixed sample with its dynamic behavior recorded prior to fixation using light microscopic approaches. This electron microscopy core unit will supply electron microscopy services for the program including sample preparation and analysis by a variety of electron microscopy techniques. The core will also develop advanced techniques in correlative light and electron microscopy specifically suited for the investigation of molecular motors and vesicle trafficking in cells and in cell-free motility systems. These new techniques will be also made available to the core users. RELEVANCE (Seeinstructions): Electron microscopy is a unique tool to get insight into fine structural organization of tissues, cells, and molecules. Such structural information paves a road toward understanding of complex biological processes, without which it is not possible to fight a disease. PROJECT/