Correlative and Advanced Electron Microscopy

相关和先进电子显微镜

• 批准号: 8063033
• 负责人: Tatyana Svitkina
• 金额: $ 12.13万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8063033
• 关键词: Behavior; Biological; Biological Preservation; Biological Process; Biology; Biomedical Research; Carbon; Cells; Complex; Cytoskeleton; Diamond; Diffusion; Disease; Electron Microscope; Electron Microscopy; Electron Microscopy Facility; Equipment; Event; Film; Goals; Imagery; Individual; Instruction; Intracellular Membranes; Investigation; Life; Light; Lighting; Macromolecular Complexes; Metals; Microscope; Microscopic; Microscopy; Microtomy; Modification; Molecular; Molecular Motors; Monitor; Organelles; Plasma; Plastic Embedding; Platinum; Preparation; Principal Investigator; Procedures; Pump; Research Personnel; Resolution; Sampling; Services; Shadowing (Histology); Solutions; Source; Staging; System; Techniques; Technology; Thick; Tissues; Vacuum; Vesicle; Water; Work; cell motility; digital; electron tomography; evaporation; fighting; fundamental research; insight; instrument; instrumentation; light microscopy; member; photoactivation; programs; research study; sample fixation; success; three dimensional structure; tool; trafficking; transmission process

Program Director/Principal Investigator (Last, First, Middle): Goldman, Yale E. (Svitkina-Core C) PROJECT SUMMARY (See instructions): As fundamental research in biology increasingly focuses on events occurring at the macromolecular level, structural studies at molecular and supramolecular levels become especially important. Electron microscopy provides high resolution structural information, which exceeds in resolution and a span of applications even most advanced light microscopic approaches. The major limitation of electron microscopy, its applicability only to "dead" samples, can be partially overcome by correlating the structural organization of a fixed sample with its dynamic behavior recorded prior to fixation using light microscopic approaches. This electron microscopy core unit will supply electron microscopy services for the program including sample preparation and analysis by a variety of electron microscopy techniques. The core will also develop advanced techniques in correlative light and electron microscopy specifically suited for the investigation of molecular motors and vesicle trafficking in cells and in cell-free motility systems. These new techniques will be also made available to the core users. RELEVANCE (Seeinstructions): Electron microscopy is a unique tool to get insight into fine structural organization of tissues, cells, and molecules. Such structural information paves a road toward understanding of complex biological processes, without which it is not possible to fight a disease. PROJECT/

项目主任/主要研究者（最后，第一，中间）：高盛，耶鲁大学E。（Svitkina-Core C） 项目总结（见说明）： 随着生物学基础研究越来越关注大分子水平上发生的事件， 在分子和超分子水平上的结构研究变得特别重要。电子显微镜 提供高分辨率的结构信息，其在分辨率和应用范围上甚至超过 最先进的光学显微镜方法。电子显微镜的主要局限性， 只有“死”的样品，可以部分克服相关的结构组织的固定 在固定之前使用光学显微镜方法记录样品的动态行为。该电子 显微镜核心单位将提供电子显微镜服务的计划，包括样品制备 并通过各种电子显微镜技术进行分析。核心还将开发先进的 相关光学和电子显微镜技术，特别适合于分子的研究 马达和囊泡运输细胞和无细胞运动系统。这些新技术也将 提供给核心用户。 相关性（参见说明）： 电子显微镜是一种独特的工具，可以深入了解组织，细胞和细胞的精细结构组织。 分子。这种结构信息为理解复杂的生物过程铺平了道路， 没有它就不可能战胜疾病。 项目/