Structure and functions of the actin cytoskeleton

肌动蛋白细胞骨架的结构和功能

• 批准号: 10794592
• 负责人: Tatyana Svitkina
• 金额: $ 25万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-16 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10794592
• 关键词: Actins; Administrative Supplement; Cytoskeleton; Equipment; Fluorescence; Funding; Goals; Image; Lighting; Microscope; Microscopy; Productivity; Research; Structure; Technology; fighting; human disease; novel diagnostics; parent grant; tool; ultra high resolution

Summary In this Administrative Supplement for Equipment application I request funds to purchase a superresolution fluorescence module (DeepSIM) installed on Nikon inverted microscope to perform subdiffraction imaging using SIM (Structured Illumination Microscopy) technology in order to perform research at the high technological level demanded by the current state of the field. This equipment is needed to achieve goals of the parent grant R35 GM 140832 entitled “Structure and functions of the actin cytoskeleton”.

总结 在这份设备申请的行政补充文件中，我申请资金购买一台超分辨率 荧光模块（DeepSIM）安装在尼康倒置显微镜上，用于进行亚衍射成像 使用SIM（结构化照明显微镜）技术，以进行研究，在高 技术水平要求的领域。需要这种设备来实现以下目标： 标题为“肌动蛋白细胞骨架的结构和功能”的母授权R35 GM 140832。

项目成果

Feedback between mechanosensitive signaling and active forces governs endothelial junction integrity.

• DOI: 10.1038/s41467-022-34701-y
• 发表时间: 2022-11-19
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: McEvoy, Eoin;Sneh, Tal;Moeendarbary, Emad;Javanmardi, Yousef;Efimova, Nadia;Yang, Changsong;Marino-Bravante, Gloria E.;Chen, Xingyu;Escribano, Jorge;Spill, Fabian;Manuel Garcia-Aznar, Jose;Weeraratna, Ashani T.;Svitkina, Tatyana M.;Kamm, Roger D.;Shenoy, Vivek B.
• 通讯作者: Shenoy, Vivek B.

Actin polymerization promotes invagination of flat clathrin-coated lattices in mammalian cells by pushing at lattice edges.

• DOI: 10.1038/s41467-022-33852-2
• 发表时间: 2022-10-17
• 期刊: Nature communications
• 影响因子: 16.6

Adenomatous Polyposis Coli (APC) in cell migration.

• DOI: 10.1016/j.ejcb.2022.151228
• 发表时间: 2022-06
• 期刊: EUROPEAN JOURNAL OF CELL BIOLOGY
• 影响因子: 6.6
• 作者: Fang, Xingyuan;Svitkina, Tatyana M.
• 通讯作者: Svitkina, Tatyana M.

A phosphoinositide switch mediates exocyst recruitment to multivesicular endosomes for exosome secretion.

磷酸肌醇开关介导外囊肿募集到多囊内体进行外泌体分泌。

• DOI: 10.1038/s41467-023-42661-0
• 发表时间: 2023-10-28
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Liu, Di-Ao;Tao, Kai;Wu, Bin;Yu, Ziyan;Szczepaniak, Malwina;Rames, Matthew;Yang, Changsong;Svitkina, Tatyana;Zhu, Yueyao;Xu, Fengyuan;Nan, Xiaolin;Guo, Wei
• 通讯作者: Guo, Wei

Imaging Cytoskeleton Components by Electron Microscopy.

• DOI: 10.1007/978-1-0716-1661-1_2
• 发表时间: 2022
• 期刊: Methods in molecular biology (Clifton, N.J.)