Molecular Genetics of HSV Reactivation
HSV 再激活的分子遗传学
基本信息
- 批准号:8187898
- 负责人:
- 金额:$ 41.01万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-08-15 至 2015-07-31
- 项目状态:已结题
- 来源:
- 关键词:Afferent NeuronsAntiviral AgentsBerylliumBindingBlindnessCellsChromatinChromosomesClinicalComplexDNADataDevelopmentDiseaseEZH2 geneElementsEncephalitisEpigenetic ProcessEpisomeEventFunctional RNAGangliaGene SilencingGenesGenetic TranscriptionGenomeGoalsHerpes LabialisHerpesvirus 1HeterochromatinHistone H3HumanImmunoprecipitationInfectionIntronsLifeLysineLyticMaintenanceMalignant NeoplasmsMapsMediatingModelingMolecular GeneticsMusNeuronsPRC1 ProteinPlayPolycombProcessProteinsRNARecruitment ActivityRecurrenceRegulationRelative (related person)RepressionResearchRoleSimplexvirusSmall Interfering RNASpecificityStimulusStructureTestingViralViral GenomeVirus LatencyWorkYY1 Transcription Factorcell growth regulationflexibilityinhibitor/antagonistinsightknock-downlatent infectionlytic gene expressionmutantnovelpromoterprotein complexresearch studysmall molecule
项目摘要
DESCRIPTION (provided by applicant): Herpes simplex virus type 1 (HSV-1) establishes a life-long latent infection within sensory neurons. During latency the viral genomes are maintained as circular episomes and the lytic genes are silenced. Periodically the genomes within some of the neurons reactivate resulting in recurrent clinical disease. A major focus of our research is to determine how HSV-1 lytic genes are silenced in neurons during latency, and how this process is reversed during reactivation. During the past project period our studies have implicated the polycomb repressor complex (PRC) as an important epigenetic regulator of HSV latency and reactivation. The overall hypothesis to be tested in this proposal is that viral regulation of PRC binding to the HSV genome plays a key role in controlling the degree of suppression of lytic genes during latency in a manner that facilitates reactivation. The experiments proposed here will define the viral elements that are involved in this process. In Aim 1 we will identify the regions of the HSV-1 genome that recruit the polycomb establishment complex (PRC2). Cellular genes silenced by H3K27triMe recruit PRC2 via cis DNA elements or indirectly through interactions with YY1 or non-coding RNAs (ncRNAs). PRC2 then methylates histone H3 at lysine 27 which leads to heterochromatin formation. Therefore we will determine if PRC2 binds directly to elements on the HSV-1 genome, or whether this binding is mediated through other factors. After heterochromatin is established a second PRC, the PRC maintenance complex (PRC1), recognizes the H3K27triMe mark and maintains the repressed state. RNA immunoprecipitation (RIP) data indicate that the LAT 2.0kb stable intron binds to the PRC1 suggesting that the intron sequesters PRC1 and reduces the degree of H3K27triMe repression on the latent genomes. We hypothesize that this competition with PRC1 binding is a critical factor in maintaining the lytic genes in a more flexible "suppressed but reversible" heterochromatic state facilitating reactivation. In Aim 2 we will characterize the binding of PRC1 to the LAT intron and determine if reducing the amount of PRC1 in the cell is suficient to enhance HSV reactivation. Finally, in order for reactivation to occur, the PRC1 repression must be released and the chromatin associated with the HSV-1 lytic promoters must remodel to a transcriptionaly permissive state. We have previously shown that during explant-induced reactivation of latent murine ganglia that there is a transient increase in LAT abundance. The goal of Aim 3 will be to determine how the H3K27triMe mark is removed in order to allow lytic transcription to proceed, and whether the LAT is directly involved in this process. The proposed studies will provide key insight into the mechanism of lytic gene silencing during HSV-1 latency and details concerning the novel role that the LAT intron plays in modulating H3K27triMe. In addition this work may provide new insight into novel mechanisms of PRC regulation of cellular genes.
PUBLIC HEALTH RELEVANCE: Herpes simplex virus (HSV) causes cold sores and other serious disease in humans including encephalitis and blindness. While there are antiviral drugs to treat herpes, they don't completely block the infection and there is no cure. This research is aimed at studying a cellular protein complex, PRC, involved in controlling HSV genes during latent infection and could result in identifying new targets to develop better therapies for treating herpes infections.
描述(由申请人提供):1型单纯疱疹病毒(HSV-1)在感觉神经元内建立终身潜伏感染。在潜伏期,病毒基因组保持为环状附加体,裂解基因沉默。一些神经元内的基因组定期重新激活,导致临床疾病复发。我们研究的一个主要重点是确定HSV-1裂解基因在潜伏期期间如何在神经元中沉默,以及这一过程在重新激活期间如何逆转。在过去的项目期间,我们的研究表明多梳阻遏复合物(PRC)作为一个重要的HSV潜伏期和再激活的表观遗传调节因子。在该提议中待检验的总体假设是,病毒调节PRC与HSV基因组的结合在以促进再激活的方式控制潜伏期期间裂解基因的抑制程度方面起关键作用。这里提出的实验将确定参与这一过程的病毒元件。在目的1中,我们将鉴定HSV-1基因组中招募多梳建立复合体(PRC 2)的区域。被H3 K27 triMe沉默的细胞基因通过顺式DNA元件或间接通过与YY 1或非编码RNA(ncRNA)的相互作用招募PRC 2。然后PRC 2在赖氨酸27处甲基化组蛋白H3,这导致异染色质形成。因此,我们将确定PRC 2是否直接与HSV-1基因组上的元件结合,或者这种结合是否通过其他因素介导。在异染色质形成后,第二个PRC,PRC维持复合物(PRC 1),识别H3 K27 triMe标记并维持抑制状态。RNA免疫沉淀(RIP)数据表明LAT 2.0kb稳定内含子与PRC 1结合,表明内含子隔离PRC 1并降低H3 K27 triMe对潜伏基因组的抑制程度。我们假设,与PRC 1结合的这种竞争是将裂解基因维持在更灵活的“抑制但可逆”异染色质状态以促进重新激活的关键因素。在目的2中,我们将表征PRC 1与LAT内含子的结合,并确定减少细胞中PRC 1的量是否足以增强HSV再活化。最后,为了使再激活发生,PRC 1抑制必须被释放,并且与HSV-1裂解启动子相关的染色质必须重塑到转录允许状态。我们以前已经表明,在潜伏的小鼠神经节,有一个短暂的LAT丰度增加,在QT诱导的再激活。目标3的目标是确定如何去除H3 K27 triMe标记以允许裂解转录进行,以及LAT是否直接参与该过程。拟议的研究将提供关键的洞察力裂解基因沉默的机制,在HSV-1潜伏期和细节的新作用,LAT内含子在调制H3 K27 triMe。此外,这项工作可能会提供新的见解,PRC的细胞基因调控的新机制。
公共卫生相关性:单纯疱疹病毒(HSV)可引起唇疱疹和其他严重的人类疾病,包括脑炎和失明。虽然有抗病毒药物治疗疱疹,但它们不能完全阻断感染,也没有治愈方法。这项研究的目的是研究一种细胞蛋白复合物PRC,它在潜伏感染期间参与控制HSV基因,并可能导致确定新的靶点,以开发更好的治疗疱疹感染的疗法。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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David C. Bloom其他文献
Phosphorylated-tau associates with HSV-1 chromatin and correlates with nuclear speckles decondensation in low-density host chromatin regions
磷酸化tau 与单纯疱疹病毒 1 染色质相关联,并与低密度宿主染色质区域的核斑点解凝聚相关。
- DOI:
10.1016/j.nbd.2025.106804 - 发表时间:
2025-03-01 - 期刊:
- 影响因子:5.600
- 作者:
Leonardo D'Aiuto;Jill K. Caldwell;Terri G. Edwards;Chaoming Zhou;Matthew L. McDonald;Roberto Di Maio;Wood A. Joel;Vanesa R. Hyde;Callen T. Wallace;Simon C. Watkins;Maribeth A. Wesesky;Or A. Shemesh;Vishwajit L. Nimgaonkar;David C. Bloom - 通讯作者:
David C. Bloom
Herpes simplex virus-1 and varicella-zoster virus latency in ganglia
- DOI:
10.1080/13550280390194000 - 发表时间:
2003-03-01 - 期刊:
- 影响因子:1.900
- 作者:
Bradley M. Mitchell;David C. Bloom;Randall J. Cohrs;Donald H. Gilden;Peter G. E. Kennedy - 通讯作者:
Peter G. E. Kennedy
801. RNA Gene Therapy Targeting Herpes Simplex Virus
- DOI:
10.1016/j.ymthe.2006.08.890 - 发表时间:
2006-01-01 - 期刊:
- 影响因子:
- 作者:
Jia Liu;Sonal S. Tuli;David C. Bloom;Gregory S. Schultz;Steve C. Ghivizzani;Alfred S. Lewin - 通讯作者:
Alfred S. Lewin
Posterior ankyloglossia: A case report
- DOI:
10.1016/j.ijporl.2009.02.011 - 发表时间:
2009-06-01 - 期刊:
- 影响因子:
- 作者:
Michael W. Chu;David C. Bloom - 通讯作者:
David C. Bloom
David C. Bloom的其他文献
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{{ truncateString('David C. Bloom', 18)}}的其他基金
Effects of HSV-1 infection on neural progenitor cell biology in vitro and in vivo
HSV-1感染对神经祖细胞体外和体内生物学的影响
- 批准号:
10201788 - 财政年份:2020
- 资助金额:
$ 41.01万 - 项目类别:
Effects of HSV-1 infection on neural progenitor cell biology in vitro and in vivo
HSV-1感染对神经祖细胞体外和体内生物学的影响
- 批准号:
10623148 - 财政年份:2020
- 资助金额:
$ 41.01万 - 项目类别:
Effects of HSV-1 infection on neural progenitor cell biology in vitro and in vivo
HSV-1感染对神经祖细胞体外和体内生物学的影响
- 批准号:
10047416 - 财政年份:2020
- 资助金额:
$ 41.01万 - 项目类别:
Effects of HSV-1 infection on neural progenitor cell biology in vitro and in vivo
HSV-1感染对神经祖细胞体外和体内生物学的影响
- 批准号:
10395571 - 财政年份:2020
- 资助金额:
$ 41.01万 - 项目类别:
Effects of HSV-1 reactivation from latency on aspects of neural precursor cells neurogenesis and accumulation of Alzheimer's molecular hallmarks
HSV-1从潜伏期重新激活对神经前体细胞神经发生和阿尔茨海默病分子标志积累的影响
- 批准号:
10710940 - 财政年份:2020
- 资助金额:
$ 41.01万 - 项目类别:
Function of histone chaperones in HSV-1 chromatin sturcture during latency, establishing maintenance and reactivation
潜伏期 HSV-1 染色质结构中组蛋白伴侣的功能、建立维持和重新激活
- 批准号:
8930277 - 财政年份:2015
- 资助金额:
$ 41.01万 - 项目类别:
Regulation of lytic and latent infection by HSV-1 encoded miRNAs
HSV-1 编码的 miRNA 对裂解和潜伏感染的调节
- 批准号:
8219674 - 财政年份:2012
- 资助金额:
$ 41.01万 - 项目类别:
Regulation of lytic and latent infection by HSV-1 encoded miRNAs
HSV-1 编码的 miRNA 对裂解和潜伏感染的调节
- 批准号:
8414420 - 财政年份:2012
- 资助金额:
$ 41.01万 - 项目类别:
Regulation of lytic and latent infection by HSV-1 encoded miRNAs
HSV-1 编码的 miRNA 对裂解和潜伏感染的调节
- 批准号:
8602830 - 财政年份:2012
- 资助金额:
$ 41.01万 - 项目类别:
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