MOLECULAR ANALYSIS OF PLASMODIUM VIVAX SURFACE ANTIGENS

间日疟原虫表面抗原的分子分析

• 批准号: 8357395
• 负责人: MARY R GALINSKI
• 金额: $ 4.12万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357395
• 关键词: Adhesions; Apical; Bioinformatics; Biological; Biological Assay; Cells; Experimental Models; Funding; Genes; Grant; Human; Image; In Vitro; Infection; Intervention; Investigation; Knowledge; Laboratories; Malaria; Malaria Vaccines; Methods; Molecular; Molecular Analysis; Monkeys; National Center for Research Resources; Parasites; Pharmaceutical Preparations; Plasmodium; Plasmodium vivax; Primates; Principal Investigator; Process; Proteins; Proteomics; Public Health; Research; Research Infrastructure; Resolution; Resources; Saimiri; Series; Source; Surface Antigens; System; Technology; Testing; Transfection; United States National Institutes of Health; Vivax Malaria; cost; genetic manipulation; genome database; merozoite surface protein; nonhuman primate; novel; programs; research study; socioeconomics; vaccine candidate

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The long term objective of this project is to analyse and understand structurally and functionally the molecular make-up of Plasmodium vivax merozoites. This knowledge will identify and characterize proteins that may serve as important vivax malaria vaccine candidates or targets of anti-malaria drug interventions. Plasmodium vivax is a highly predominant malaria species, causing 80 million cases or more of malaria each year and major socioeconomic public health ramifications worldwide. Nevertheless, P. vivax is under investigated in part because it cannot be cultured reliably long term in vitro. Specialized expertise and non human primate resources are therefore required for in depth laboratory investigations on this human parasite species. Experimental models using P. vivax parasites and the related simian malaria species P. knowlesi and P. cynomolgi in non-human primates are fundamental to this research program. This year, studies focused on confirming that 10 of 11 P. vivax (Sal I) msp3 genes are expressed as merozoite surface proteins in an infection, and that surprisingly one of these may encode a novel merozoite apical localized protein. Other efforts focused on developing and testing a series of constructs to establish transfection systems for studies of P. vivax. Additionally, parasite stocks of P. vivax were generated from Saimiri boliviensis monkey infections and processed for specific proteomic and cell biological experiments. This research capitalizes extensively on the recent availability of Plasmodium genome databases, utilizing bioinformatics, microarrays, proteomics, genetic manipulation strategies, high resolution imaging capabilities, functional adhesion and invasion assays, immunochemical methods and cell biological technologies.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 该项目的长期目的是在结构和功能上分析和理解体内植物的分子组成。这些知识将识别和表征蛋白质，这些蛋白质可能是重要的抗麦芽疟疾疫苗或抗马拉里亚药物干预措施的靶标。疟原虫是一种高度的疟疾物种，每年造成8000万例或更多的疟疾病例，并且在全球范围内引起重大的社会经济公共卫生后果。然而，Vivax的部分研究部分是因为不能在体外长期可靠地培养它。 因此，对这种人类寄生虫物种进行深度实验室调查需要专业知识和非人类灵长类动物资源。 使用Vivax P.寄生虫和相关的邻虫疟疾物种P. knowlesi和P. cynomolgi中的实验模型是该研究计划的基础。 今年，研究重点是确认11个P. Vivax（SAL I）MSP3基因在感染中以梅洛唑岩表面蛋白的形式表达，而令人惊讶的是，其中一种可能会编码一种新型的Merozoite顶端局部蛋白质。其他工作重点是开发和测试一系列构造，以建立用于维瓦克斯研究的转染系统。 此外，在saimiri boliviensis猴子感染中产生了疟原虫的寄生虫库存，并处理特定的蛋白质组学和细胞生物学实验。这项研究大量利用了疟原虫基因组数据库的最新可用性，利用生物信息学，微阵列，蛋白质组学，遗传操纵策略，高分辨率成像能力，功能粘附和入侵测定，免疫化学方法和细胞生物学技术。