A LIMBIC CIRCUIT ANALYSIS OF DEEP BRAIN STIMULATION FOR DEPRESSION

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 2010年，我们继续致力于目标1：表征BLA和PFC中的自发活动，并评估同步振荡的存在。 我们已经从六只接受恐惧条件反射和消除训练的老鼠身上收集了数据，并开始撰写描述我们发现的手稿。 我们已经证明，BLA和mPFC的局部场电位中的δ（1.5- 4 Hz）和γ（30- 80 Hz）频带在恐惧学习和表达过程中显示出独特的增强一致性模式，并且这些模式在没有学习恐惧关联的大鼠中没有观察到，表明与动物的情绪状态存在功能关系。 我们现在已经开始了与目标2相关的实验：评估PFC刺激的神经和行为反应。 我们应用电刺激模拟人类DBS参数的mPFC区域被认为是同源的Cg 25，同时记录行为和神经反应的BLA。 通过在恐惧消退训练方案中进行刺激，我们希望证明负面情绪的抑制和消退学习的加速。 本实验中使用的大鼠刚刚抵达，每天都会称重，为限食做准备，这是该方案的第一步。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In 2010, we continued work on Aim 1: Characterize spontaneous activity in the BLA and PFC, and evaluate the presence of synchronous oscillations. We have collected data from six rats undergoing fear conditioning and extinction training, and have begun work on a manuscript describing our findings. We have demonstrated that delta (1.5-4Hz) and gamma (30-80Hz) frequency bands in the local field potentials of the BLA and mPFC show unique patterns of increased coherence during fear learning and expression, and that these patterns are not seen in rats that do not learn the fear association, indicating a functional relationship with the emotional state of the animal. We have now begun experiments related to Aim 2: Evaluate neural and behavioral response to PFC stimulation. We have applied electrical stimulation modeled after human DBS parameters to the mPFC region thought to be homologous to Cg25, while recording behavior and neural responses in the BLA. By stimulating during the fear extinction training protocol, we hope to demonstrate a suppression of negative emotion and an acceleration of extinction learning. The rats being used in this experiment have just arrived and are being weighed daily in preparation for food restriction, the first step in the protocol.