A LIMBIC CIRCUIT ANALYSIS OF DEEP BRAIN STIMULATION FOR DEPRESSION

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 2010年，我们继续了目标1的工作：描述BLA和PFC中的自发活动，并评估同步振荡的存在。我们已经收集了六只正在接受恐惧条件作用和消退训练的大鼠的数据，并已开始撰写一份描述我们发现的手稿。我们已经证明，在恐惧学习和表达过程中，BLA和mPFC局部场势中的增量(1.5-4赫兹)和伽马(30-80赫兹)频段显示出独特的连贯性增强模式，并且这些模式在没有学习恐惧联系的大鼠中看不到，这表明这些模式与动物的情绪状态存在功能关系。 我们现在已经开始了与目标2相关的实验：评估对PFC刺激的神经和行为反应。我们仿照人类DBS参数对被认为与Cg25同源的mPFC区域进行了电刺激，同时记录了BLA的行为和神经反应。通过在恐惧消退训练方案中进行刺激，我们希望展示一种对负面情绪的抑制和消退学习的加速。这项实验中使用的大鼠刚刚到达，每天都要称重，为食物限制做准备，这是协议的第一步。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In 2010, we continued work on Aim 1: Characterize spontaneous activity in the BLA and PFC, and evaluate the presence of synchronous oscillations. We have collected data from six rats undergoing fear conditioning and extinction training, and have begun work on a manuscript describing our findings. We have demonstrated that delta (1.5-4Hz) and gamma (30-80Hz) frequency bands in the local field potentials of the BLA and mPFC show unique patterns of increased coherence during fear learning and expression, and that these patterns are not seen in rats that do not learn the fear association, indicating a functional relationship with the emotional state of the animal. We have now begun experiments related to Aim 2: Evaluate neural and behavioral response to PFC stimulation. We have applied electrical stimulation modeled after human DBS parameters to the mPFC region thought to be homologous to Cg25, while recording behavior and neural responses in the BLA. By stimulating during the fear extinction training protocol, we hope to demonstrate a suppression of negative emotion and an acceleration of extinction learning. The rats being used in this experiment have just arrived and are being weighed daily in preparation for food restriction, the first step in the protocol.