PROMOTER-BASED FUNCTIONAL MAPPING OF AMYGDALA MICROCIRCUITS

基于启动子的杏仁核微电路功能图谱

• 批准号: 8172377
• 负责人: DONALD G RAINNIE
• 金额: $ 4.39万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172377
• 关键词: Amygdaloid structure; Cholecystokinin; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Green Fluorescent Proteins; Institution; Interneurons; Investigation; Maps; Neurons; Physiological; Property; Rattus; Reporting; Research; Research Personnel; Resources; Source; United States National Institutes of Health; base; in vivo; promoter

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During the reporting period we completed our investigation of the physiological and morphological properties of cholecystokinin (CCK) containing interneurons using the CCK promoter tagged with a green fluorescent protein (GFP). The resulting lentiviral construct was then used to transfect neurons in vivo, only those neurons that normally express CCK co-expressed GFP. We have used this approach to examine the physiological properties of CCK interneurons in the rat basolateral amygdala.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在报告期间，我们利用绿色荧光蛋白(GFP)标记的CCK启动子，完成了对含有CCK的中间神经元的生理和形态特性的研究。 然后将所得到的慢病毒构建物用于体内神经元的转基因，只有那些正常表达CCK的神经元才共同表达GFP。我们用这种方法研究了大鼠杏仁基底外侧核CCK中间神经元的生理特性。