IMMUNO-VIROLOGICAL AND SAMPLE REPOSITORY SIV-INFECTED AND UNINFECTED MANGABEYS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 本管理研究将感染SIV和未感染SIV的白眉猴（SM）的Yerkes菌落作为一个队列，进行一年两次的免疫病毒学评价。从2004年开始每年两次的样本采集，在2004 - 2009年期间采集了500多份外周血样本。对这些样本进行了完整的分析，以测量病毒载量和28个免疫学参数。 这些信息，以及血浆和PBMC冷冻样本的储存库，可根据要求提供给Yerkes和非Yerkes研究者用于选定的研究项目。从这个项目中使用的样品已经在该领域的几个重要的出版物，因为这种方法产生了大量的免疫学和病毒学数据进行系统的纵向评估的动物的健康状况，特别是SIV感染的过程中的工具。值得注意的是，第三次动物调查于2009年完成，相关的免疫学和病毒学数据发表在Taaffe et al.，J Virol 2010. 这组数据还允许快速有效地鉴定具有特异性免疫学和/或病毒学特征的SM，并与完整的SM菌落谱系相匹配。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This management study treats Yerkes' colonies of SIV-infected and uninfected sooty mangabeys (SMs) as cohorts for a biannual Immuno-virological evaluation. The biannual sample collections began in 2004 and between the years of 2004-2009 more than 500 peripheral blood samples were collected. A complete analysis has been conducted on these samples for measurement of viral load and 28 immunological parameters. This information, and the repository of plasma and PBMC frozen samples, are made available for selected research projects to both Yerkes and non-Yerkes investigators upon request. The samples utilized from this project have been instrumental to several important publications in the field, since this approach generated a large set of immunological and virological data to conduct as systematic longitudinal assessment of the animals' health status with specific focus on the course of SIV infection. Of note, the third survey of the animals was completed in 2009, and the relevant immunological and virological data were published in Taaffe et al., J Virol 2010. This set of data has also allowed the rapid and effective identification of SMs with specific immunological and or virological features, and to match against the completed SM colony pedigree.