Vanberbilt Biomarker Developmental Laboratory

范伯比尔特生物标志物发育实验室

• 批准号: 8132582
• 负责人: Daniel C Liebler
• 金额: $ 63.7万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-20 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8132582
• 关键词: Address; Algorithms; Biological; Biological Assay; Biological Markers; Blood; Cancer Center; Characteristics; Clinical; Collection; Colorectal Cancer; Complex; Computer software; Coupled; Data Set; Detection; Development; Diagnosis; Diagnostic tests; Disease; Early Detection Research Network; Early Diagnosis; Equipment and supply inventories; Experimental Designs; Funding; Genomics; Goals; Informatics; Institutes; Laboratories; Learning; Link; Liquid Chromatography; Malignant Neoplasms; Malignant neoplasm of lung; Mass Spectrum Analysis; Methods; Mutation; Normal tissue morphology; Pathway Analysis; Peptides; Performance; Phase; Phenotype; Plasma; Post-Translational Protein Processing; Process; Proteins; Proteome; Proteomics; Research Infrastructure; Resected; Resources; Screening for cancer; Shotguns; Signal Transduction; Source; Specimen; Technology; Technology Assessment; Thoracic Oncology; Tissues; Tumor Tissue; Validation; Variant; Work; base; colorectal cancer screening; instrumentation; multiple reaction monitoring; mutant; novel; oncology program; programs; tandem mass spectrometry; tool; tumor

This application proposes a Vanderbilt Biomarker Developmental Laboratory (Vanderbilt BDL), which will serve as a component of the NCI Early Detection Research Network (EDRN). The Vanderbilt BDL will be established within the Jim Ayers Institute for Precancer Detection and Diagnosis in the Vanderbilt-lngram Cancer Center which provides outstanding resources, instrumentation and staff dedicated to the problem of biomarker development. The Vanderbilt BDL will employ standardized liquid chromatography-tandem mass spectrometry (LC-MS/MS) and liquid chromatography-multiple reaction monitoring mass spectrometry (LC-MRM-MS) analysis platforms for unbiased discovery and targeted quantitation of protein biomarkers. These analysis platforms are coupled to extensive informatics support infrastructure and ongoing biospecimen collections of the Ayers Institute and the Thoracic Oncology Program in the Vanderbilt-lngram Cancer Center. The Vanderbilt BDL will address the goals of the EDRN BDL program through three specific aims, 1) Develop and apply new informatics tools and approaches to identify mutant, variant and modified proteins as candidate cancer biomarkers. This work will emphasize application of new sequence tagging and signal analysis algorithms and software to identify novel protein forms that distinguish cancer phenotypes, 2) Discover and verify protein biomarker candidates for plasma-based detection of colorectal and lung cancers. This work will apply standardized shotgun proteomics analyses for biomarker candidate discovery in tissues and a standardized LC- MRM-MS platform for quantitation of biomarker candidates in plasma. The Vanderbilt BDL will interact closely with a proposed EDRN Biomarker Reference Laboratory (William Pao, PI) and a proposed EDRN Clinical Validation Center (Pierre Massion, PI) at Vanderbilt as well as with EDRN network partners to broadly deploy standardized proteomic technologies for biomarker development.

该申请提出了一个范德比尔特生物标志物开发实验室（范德比尔特BDL），它将作为NCI早期检测研究网络（EDRN）的一个组成部分。范德比尔特BDL将在范德比尔特-英格拉姆癌症中心的Jim Ayers癌前检测和诊断研究所内建立，该研究所提供杰出的资源、仪器和工作人员，致力于生物标志物的开发。范德比尔特BDL将采用标准化液相色谱-串联质谱（LC-MS/MS）和液相色谱-多反应监测质谱（LC-MRM-MS）分析平台，用于蛋白质生物标志物的无偏发现和靶向定量。这些分析平台与艾尔斯研究所和范德比尔特-英格拉姆癌症中心的胸部肿瘤学项目的广泛的信息学支持基础设施和正在进行的生物标本收集相结合。范德比尔特BDL将通过三个具体目标实现EDRN BDL计划的目标，1）开发和应用新的信息学工具和方法，以识别突变，变异和修饰的蛋白质作为候选癌症生物标志物。这项工作将强调新的序列标记和信号分析算法和软件的应用，以识别区分癌症表型的新型蛋白质形式，2）发现和验证用于基于血浆的结直肠癌和肺癌检测的蛋白质生物标志物候选物。这项工作将应用标准化的鸟枪蛋白质组学分析来发现组织中的生物标志物候选物，并应用标准化的LC-MRM-MS平台来定量血浆中的生物标志物候选物。范德比尔特BDL将与拟议的EDRN生物标志物参考实验室（William Pao，PI）和拟议的EDRN临床验证中心（Pierre Massion，PI）以及EDRN网络合作伙伴密切互动，以广泛部署标准化蛋白质组学技术用于生物标志物开发。