Multifunction High Throughput System for Neurophysiology of Behavior

行为神经生理学多功能高通量系统

• 批准号: 8006362
• 负责人: DONALD JAY WOODWARD
• 金额: $ 29.9万
• 依托单位: BIOGRAPHICS, INC.
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-15 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8006362
• 关键词: Achievement; Alcohol abuse; Alcoholism; Algorithms; Animal Behavior; Animals; Antipsychotic Agents; Area; Behavior; Behavior Control; Behavioral; Brain; Brain Injuries; Brain Pathology; Brain region; Caliber; Client; Cognition; Communication; Complex; Computer software; Computers; Data; Data Collection; Data Set; Databases; Development; Disease; Drug Evaluation; Drug abuse; Drug effect disorder; Electronics; Epilepsy; Equipment; Evaluation; Evaluation Research; Future; Genetic Models; Goals; Housing; Individual; Infusion Pumps; Infusion procedures; Intravenous infusion procedures; Laboratories; Lead; Life; Liquid substance; Longitudinal Studies; Memory; Motor; Movement; Mus; National Institute of Drug Abuse; National Institute of Mental Health; National Institute of Neurological Disorders and Stroke; National Institute on Alcohol Abuse and Alcoholism; Neurons; Optics; Output; Pain; Performance; Pharmaceutical Preparations; Phase; Physiology; Population; Procedures; Protocols documentation; Psyche structure; Pump; Rattus; Recovery; Recovery of Function; Research; Rodent; Schizophrenia; Seizures; Services; Signal Transduction; Sleep; Sleep Disorders; Small Business Innovation Research Grant; Sorting - Cell Movement; Speed; Stimulus; Syringes; System; Technology; Testing; Therapeutic; Therapeutic Agents; Time; Tube; Wireless Technology; Work; advanced system; aging brain; base; brain research; computerized data processing; cost; cost effective; cost effectiveness; data acquisition; design; digital; experience; high throughput technology; indexing; innovation; instrument; instrumentation; interstitial; mange; neurophysiology; new technology; novel; prototype; relating to nervous system; relational database; research study; response; sensor

DESCRIPTION (provided by applicant): The goal of this project is to create a system to conduct 'High Throughput Multifunction System for Neurophysiology of Behavior', in response to PA-08-001. The advanced system will provide capabilities for many other Behavioral Neurophysiology research applications for NIMH, NINDS, NIDA, NIAAA, and NIA. Multiple new technologies will be integrated to achieve a high performance high throughput system. A group of Collaborators will provide critical test and evaluation for research applications in schizophrenia, aging brain, sleep and brain states, pain modulation, seizure prediction, and alcohol abuse. A First Aim is to design and assemble a data acquisition and control module for continuous long duration studies of animals living in an experimental chamber. The planned arrangement will also allow multiple drug infusions in long term studies of therapeutic drugs. A version of a new compact low cost multichannel recording/stimulation and spike sorting electronics module will be designed based on an advanced field programmable gate array. The compact unit will be installed above an experimental chamber on a unit that will rotate by a motor system triggered by movement of the rodent. High speed wireless, optical, or digital communication from the rotating unit will allow data and control signals to pass from each experimental chamber to a host computer data acquisition service. Multichannel electrical, optical stimulation and electrochemical sensing will be integrated in efficient manner. The tethered cabling from the data acquisition system will be inserted through a large diameter aperture to the mouse or rat. Tethered instrumentation will rotate with the animal and thus allow long duration studies, as has been routine in sleep research on rodents. Installation of syringe infusion pumps on the unit that rotates with the animal is a novel design allowing for multiple fluid connections to and from the animals without use of fluid or optical swivels. This design eliminates the traditional commutator system with many expensive slip rings, and allows greatly expanded numbers of wired, semi-wireless, fluidic, and optical connections to be made with a moving animal over long times. A Second Aim is to create remote control client software to control both an existing spike sorter software module and a real time state machine module for experimental control of behavioral studies. This enhancement will allow a remote client with a single operator to control multiple workstations. A second part of this Aim is to begin use and evaluation of a data handling strategy that employs multiple time index files in parallel with an existing high speed database engine to contain all data types collected. Input and output functions will interface with Matlab scripts and templates to reduce the time required for analysis the large volumes of data. Many types of experimental control information, neurophysiological and anatomical data will be organized with use of the relational database engine. A Third Aim is to conduct evaluation studies of drugs related to therapy for multiple applications in behavioral neurophysiology related to development of therapeutic agents. Neuron population activity will be recorded in multiple brain regions with rats housed continuously over thirty days while testing responses. The overall goal of the introduction of the interrelated enhancements is to reduce the required labor and instrumentation to achieve high efficiency. The system will provide infinite expandability to mange either very large parallel recording system or multiple individual experimental chambers. This procedure will fully test basic features of the new technology developed and conduct a key proof of concept evaluation for a wide range of application in behavioral neurophysiology. PUBLIC HEALTH RELEVANCE: This project will develop an extremely efficient and cost effective instrument to control and test a wide range of behavior of animals while recording neural activity of multiple brain regions. The system includes a number of enhancements of both electronic instrumentation and the extensive software needed to enable high throughput studies. Such a system is needed to determine the actions of drugs that can cause amelioration of schizophrenia, aging brain, sleep disorder, epilepsy, alcohol and drug abuse and recovery from brain injury. The system will useful for efficient studies related to drug abuse, alcoholism, and improvement of memory, cognition in mental disease, and function in aging brain

描述（由申请人提供）：该项目的目标是根据PA-08-001的要求，创建一个用于“行为神经生理学的高通量多功能系统”的系统。该先进系统将为NIMH、NINDS、NIDA、NIAAA和NIA的许多其他行为神经生理学研究应用提供能力。将集成多种新技术以实现高性能高吞吐量系统。一组合作者将为精神分裂症、大脑老化、睡眠和大脑状态、疼痛调节、癫痫发作预测和酒精滥用的研究应用提供关键的测试和评估。第一个目标是设计和组装一个数据采集和控制模块，用于对生活在实验室内的动物进行连续、长时间的研究。计划中的安排还将允许在治疗药物的长期研究中注射多种药物。一种新型紧凑型低成本多通道记录/刺激和尖峰分选电子模块将基于先进的现场可编程门阵列进行设计。该紧凑型单元将安装在实验室上方的单元上，该单元将由啮齿动物的运动触发的电机系统旋转。旋转装置的高速无线、光学或数字通信将允许数据和控制信号从每个实验室传递到主机数据采集服务。多通道电、光刺激和电化学传感将有效地集成在一起。数据采集系统的缆绳将通过一个大直径的孔插入到小鼠或大鼠身上。拴在绳上的仪器将随着动物旋转，因此可以进行长时间的研究，就像对啮齿动物的睡眠研究一样。在与动物一起旋转的装置上安装注射器输液泵是一种新颖的设计，允许在不使用流体或光学旋转的情况下与动物进行多次流体连接。这种设计消除了带有许多昂贵滑环的传统换向器系统，并且允许在长时间内与移动的动物进行有线，半无线，流体和光学连接的数量大大增加。第二个目标是创建远程控制客户端软件来控制现有的尖峰分选软件模块和实时状态机模块，用于行为研究的实验控制。这种增强将允许一个远程客户端与一个操作员控制多个工作站。本目标的第二部分是开始使用和评估一种数据处理策略，该策略与现有的高速数据库引擎并行使用多个时间索引文件来包含收集的所有数据类型。输入和输出函数将与Matlab脚本和模板接口，以减少分析大量数据所需的时间。许多类型的实验控制信息、神经生理和解剖数据将使用关系数据库引擎进行组织。第三个目标是开展与治疗相关的药物的评估研究，用于与治疗药物开发相关的行为神经生理学的多种应用。研究人员将老鼠连续饲养30多天，记录老鼠大脑多个区域的神经元数量活动，同时测试老鼠的反应。引入相关增强功能的总体目标是减少所需的人工和仪器，以实现高效率。该系统将提供无限的可扩展性，以管理非常大的并行记录系统或多个单独的实验室。该程序将充分测试新技术的基本特征，并为行为神经生理学的广泛应用进行关键的概念验证评估。