Multifunction High Throughput System for Neurophysiology of Behavior

行为神经生理学多功能高通量系统

• 批准号: 8914474
• 负责人: DONALD JAY WOODWARD
• 金额: $ 49.99万
• 依托单位: BIOGRAPHICS, INC.
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-15 至 2017-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8914474
• 关键词: Adopted; Adoption; Alcohol abuse; Alcohol consumption; Alcohol dependence; Alcoholism; Alcohols; Amplifiers; Animal Behavior; Animals; Behavior; Behavior Control; Behavioral; Behavioral Research; Brain; Brain region; Chemical Stimulation; Chemicals; China; Client; Cognition; Collection; Communication; Complex; Computer software; Computers; Data; Data Analyses; Data Set; Detection; Development; Devices; Disease; Dopamine; Drug Addiction; Drug Evaluation; Drug abuse; Drug effect disorder; Electrochemistry; Electronics; Equipment; Ethanol; Evaluation Research; Evolution; Family; Funding; Future; Genetic Models; Goals; Head; Health; Housing; Image; Infusion Pumps; Infusion procedures; Ingestion; Intake; Intravenous infusion procedures; Investments; Laboratories; Lasers; Lead; Life; Light; Liquid substance; Measurement; Measures; Memory; Microdialysis; Mission; Motor; Movement; Mus; National Institute of Drug Abuse; National Institute of Mental Health; National Institute of Neurological Disorders and Stroke; National Institute on Alcohol Abuse and Alcoholism; Neurons; Neurosciences; Optics; Pain; Performance; Peripheral; Pharmaceutical Preparations; Phase; Phenotype; Physiologic pulse; Population; Procedures; Psyche structure; Pump; Rattus; Research; Research Activity; Rodent; Scanning; Schizophrenia; Seizures; Self Administration; Services; Signal Transduction; Site; Sleep; Small Business Innovation Research Grant; Source; Speed; Staging; Stimulus; Syringes; System; Systems Development; Technology; Testing; Therapeutic; Therapeutic Agents; Time; Tissues; Tube; Wireless Technology; Work; advanced system; aging brain; alcohol measurement; alcohol research; alcohol testing; analog; base; carbon fiber; commercialization; cost effective; cost effectiveness; data acquisition; design; digital; dopaminergic neuron; drinking; drug of abuse; experience; improved; innovation; instrument; instrumentation; mesolimbic system; miniaturize; neurophysiology; new technology; novel; parallel processing; prototype; research study; response; sensor; sensory gating; subcutaneous; validation studies

DESCRIPTION (provided by applicant): The goal of this project is to create a system to conduct research with the assistance of a 'High Throughput Multifunction System for Neurophysiology of Behavior'. This Phase II application to the Omnibus Solicitation is based on the Phase I for PA-08-001 that was funded by NIAAA 5R43AA020676-02. The advanced system will provide capabilities for many other Behavioral Neurophysiology research activity related to the missions of NIAAA, NIMH, NINDS, NIDA, and NIA. Multiple new technologies will be integrated to achieve a high performance high throughput system so that single operator can conduct multiple experiments. The main focus is to design and assemble an advanced data acquisition and control module for multiple continuous long duration studies of animals living in an experimental chamber. A rotating platform with tethered instrumentation will follow the movements of the animal. The purpose of the arrangement is to avoid the need for multiple swivel connection that limits the connections of different types that can be made with a moving animal. This arrangement will allow multiple electrical, optical and fluid connections to be made over long times. Installation of laser light sources and syringe infusion pumps on the rotating platform is a novel design. An advance data acquisition electronics module for recording and stimulation from a large number of channels will consist of an Altera Arria V GX FPGA with a PCIe connection to a PC motherboard. The multiple functions will allow continuous analog signal recording spike waveform capture, simultaneously electrochemical detection from many channels. The module also includes extensive capabilities for multiple imager support. The device is re-purposed from a DARPA that supported 'Gigapixel' camera project to enable parallel processing from hundreds of imager devices. The unique design will enable assembly of very large system configurations 10,000 or more parallel channels for future neuroscience as related technology matures. The compact unit will be installed above an experimental chamber on a unit that will rotate by a motor system triggered by movement of the rodent. The rotating system has been designed for transfer of power and control. High speed wireless, optical, or digital communication from the rotating unit will allow data and control signals to pass from each experimental chamber to a host computer data acquisition service A further aim is to advance a universal real time state machine for control of behavioral chambers and multiple devices for sensing and stimulation. Remote control client software will be created for control and analysis of data. An additional aim is to conduct evaluation studies of drugs related to therapy for multiple applications in behavioral neurophysiology related to development of therapeutic agents. Neuron population activity and multiple carbon fibers will employ fast scan electrochemistry to detect dopamine release transient in multiple brain regions with rats housed continuously over thirty days while testing responses. Microdialysis probes, multiple precision syringe pumps, camera imaging, and a colorimetric system will be adapted for repeated measurement of tissue alcohol levels in rats ingesting ethanol over time with intermittent access. Efficiency of microdialysis research will be enhanced by allowing use of multiple probes along with an improved automated collection system. A group of Collaborators to validate will provide critical and essential tests and evaluation for research applications in alcohol and drug addiction, schizophrenia, aging brain, sleep and brain states, pain modulation, and seizure prediction. The overall goal of the introduction of the interrelated enhancements is to reduce the required labor and instrumentation to achieve high efficiency for many types of behavioral research conducted simultaneously in multiple chambers, while employing a family of sensors and technologies that will be adapted for use with the rotating system. By achieving a high level of integration and efficiency the system aims to result in a System for Development of Therapeutic Drugs and Procedures'

描述（由申请人提供）：该项目的目标是创建一个系统，以进行研究的援助下，高吞吐量多功能系统的神经生理学的行为。综合征集的第二阶段申请基于由NIAAA 5 R43 AA 020676 -02资助的PA-08-001的第一阶段。先进的系统将为与NIAAA，NIMH，NINDS，NIDA和NIA任务相关的许多其他行为神经生理学研究活动提供能力。多项新技术将被整合以实现高性能高通量系统，以便单个操作员可以进行多个实验。 主要重点是设计和组装一个先进的数据采集和控制模块，用于对生活在实验室中的动物进行多个连续的长时间研究。一个带有拴系仪器的旋转平台将跟随动物的运动。该布置的目的是避免对多个旋转连接的需要，该多个旋转连接限制了可以与移动的动物进行的不同类型的连接。这种布置将允许长时间进行多个电、光和流体连接。将激光光源和注射器输液泵安装在旋转平台上是一种新颖的设计。 用于从大量通道记录和刺激的高级数据采集电子模块将由Altera Arria V GX FPGA组成，该FPGA通过PCIe连接到PC主板。多功能将允许连续的模拟信号记录尖峰波形捕获，同时从许多通道进行电化学检测。该模块还包括支持多个成像器的广泛功能。该设备是从DARPA重新设计的，该DARPA支持“千兆像素”相机项目，以实现数百个成像设备的并行处理。随着相关技术的成熟，这种独特的设计将能够组装10，000或更多并行通道的超大系统配置，用于未来的神经科学。紧凑型装置将安装在实验室上方的一个装置上，该装置将通过啮齿动物运动触发的电机系统旋转。 旋转系统的设计是为了传递动力和控制。来自旋转单元的高速无线、光学或数字通信将允许数据和控制信号从每个实验室传递到主计算机数据采集服务。进一步的目的是推进用于控制行为室和用于感测和刺激的多个设备的通用真实的时间状态机。将创建远程控制客户端软件，用于控制和分析数据。 另一个目的是对与治疗药物开发相关的行为神经生理学中多种应用的治疗相关药物进行评价研究。神经元群体活性和多个碳纤维将采用快速扫描电化学来检测多个脑区域中的多巴胺释放瞬变，其中大鼠在测试响应时连续圈养超过30天。微透析探针、多个精密注射泵、摄像成像和比色系统将适用于重复测量间歇性接触乙醇大鼠随时间的组织酒精水平。微透析研究的效率将通过允许使用多个探针沿着改进的自动收集系统来提高。一组合作者将为酒精和药物成瘾，精神分裂症，大脑老化，睡眠和大脑状态，疼痛调制和癫痫预测的研究应用提供关键和必要的测试和评估。 引入相互关联的增强功能的总体目标是减少 需要劳动力和仪器，以实现高效率的许多类型的行为研究，同时进行多个室，同时采用一系列的传感器和技术，将适用于与旋转系统。通过实现高水平的整合和效率，该系统旨在建立一个治疗药物和程序开发系统。