Ophthalmic Imaging Using Adaptive Optics and OCT

描述（由申请人提供）：本BRP的目的是开发和评估活体眼睛细胞三维成像技术，并使用这种新技术了解与导致全球失明的最常见疾病（包括年龄相关性黄斑变性和青光眼）相关的细胞层变化。四个机构的合作伙伴将致力于将提供高横向分辨率的自适应光学（AO）与提供高轴向分辨率的光学相干断层扫描（OCT）相结合的仪器。牵头机构是加州大学戴维斯分校（约翰S。Werner，PI）。在加州大学戴维斯分校和印第安纳州大学（唐纳德T。米勒，研究中心PI），将开发并测试AO-OCT仪器。杜克大学（Joseph Izatt，研究中心PI）将开发将被纳入的新型OCT方法，而劳伦斯利弗莫尔国家实验室（Scot Olivier，研究中心PI）将提供AO的前沿进展。这些AO-OCT仪器将允许具有足够分辨率和对比度的人类体内成像，以可视化人类视网膜中最小的细胞。在上一个项目期间，我们使用AO-OCT创建了以前仅在组织学上可见的结构的体积图像，包括感光细胞外段、Henle纤维、单个视神经纤维束、黄斑变性患者玻璃疣内的详细结构以及视神经筛板的精细结构。在这次更新中，我们提出解决新的技术问题，以更充分地挖掘AO-OCT的潜力和功能。工程目标是通过使用自体荧光，双折射，结合“极端AO”技术，以及与血液灌注，视网膜血管中的血红蛋白氧饱和度以及神经元细胞体积的电变化相关的功能变化来增加细胞结构的对比度。相当大的努力将致力于三维可视化和分割视网膜体积更大的横向和轴向程度比以前可能的。工程目标有平行的临床目标，以提高我们对与年龄相关性黄斑变性和青光眼标志物相关的细胞变化的理解。这些先进的成像技术将用于评估视网膜和视神经的变化，这些变化是由新的治疗方法引起的，这些新的治疗方法可以从失明的主要原因中拯救视网膜层。

DESCRIPTION (provided by applicant): The purpose of this BRP is to develop and evaluate technology for three-dimensional imaging of cells in the living eye, and to use this novel technology to understand changes in cell layers associated with the most common diseases leading to world-wide blindness, including age-related macular degeneration and glaucoma. Partners at four institutions will contribute to instrumentation that combines adaptive optics (AO), providing high lateral resolution, with optical coherence tomography (OCT), providing high axial resolution. The lead institution is the University of California, Davis (John S. Werner, PI). Both at UC Davis and at Indiana University (Donald T. Miller, site PI), AO-OCT instrumentation will be developed and tested. Duke University (Joseph Izatt, site PI) will develop novel OCT approaches that will be incorporated, while Lawrence Livermore National Laboratory (Scot Olivier, site PI) will provide cutting-edge advances in AO. These AO-OCT instruments will permit human in vivo imaging with sufficient resolution and contrast to visualize the smallest of cells in the human retina. In the previous project period we have used AO-OCT to create volume images of structures previously only visible with histology, including the photoreceptor outer segments, Fibers of Henle, individual optic nerve fiber bundles, detailed structures within drusen of macular degeneration patients, and fine structure of the lamina cribosa of the optic nerve. In this renewal, we propose to solve new technical issues to more fully tap the potential and functionality of AO-OCT. The engineering goals are directed toward increasing contrast of cellular structures by use of autofluoresence, birefringence, the incorporation of "extreme AO" techniques, and functional changes associated with blood perfusion, hemoglobin oxygen saturation in the retinal vasculature as well as electrical changes in neuronal cell volumes. Considerable effort will be devoted to three-dimensional visualization and segmentation over retinal volumes of larger lateral and axial extent than previously possible. The engineering goals have parallel clinical aims for improving our understanding of the cellular changes associated with markers for age-related macular degeneration and glaucoma. These advanced imaging techniques will be deployed for evaluating the changes in the retina and optic nerve resulting from novel therapies for rescuing retinal layers from the leading causes of blindness.