SENSORS FOR ANIONS OF ENVIRONMENTAL AND BIOMEDICAL RELEVANCE

基于硫代酰胺的阴离子传感器 环境与生物医学的相关性 阴离子的选择性结合发生在生命中的许多生理和酶过程中，它是 对许多应用至关重要，例如新传感设备的潜在原型、新的 萃取剂和生物载体。令人惊讶的是，人工合成的阴离子受体和传感器 仍然非常有限。该提案将专注于开发新的阴离子受体、粘合剂和传感器， 这将在改善人类健康和环境方面有应用。该项目的基础是 假设引入(C=S)NH作为与合成受体的结合部位，增强 阴离子结合能力，以及如果连接到分子上的荧光团显示可检测的光学 阴离子存在时的变化。该项目的长期目标是为 设计高选择性的传感器系统，从而为阴离子领域增添了一个新的维度 传感器化学。在特定目标1中，一系列新的具有不同间隔基的硫代酰胺受体 并将合成可变刚性，包括(1)带有Tren的无环硫酰胺受体， 芳香族和冠醚单元，(Ii)具有可变间隔的大环硫酰胺受体，和(Iii) 含有芘和丹磺酰基的光学传感器。系列(I)和(II)将通过 (C=S)NH…X-相互作用，而串联(III)的信号单元(S)将作为阴离子传感。特定的 目的2研究合成的分子对阴离子的结合性能。 不同的溶剂。目标阴离子是硫酸盐、磷酸盐、硝酸盐、高氯酸盐和卤化物，它们是 与环境健康和公共健康直接相关。具体目标3将重点放在隔离 与我们的合成配体结合的阴离子络合物，并确定这些络合物的结构 通过X射线结晶学研究。在具体目标4中，将研究硫代酰胺类配体的细胞毒性。 用四甲基偶氮唑盐比色法测定人肝癌细胞和人甲状腺癌细胞的DNA含量。成功 该项目的完成将极大地促进制定环境保护框架。 和生物医学应用，并为实现杰克逊州立大学RCMI的总体目标 大学。

THIOAMIDE-BASED SENSOR FOR ANIONS OF ENVIRONMENTAL AND BIOMEDICAL RELEVANCE Selective binding of anions occurs in numerous physiological and enzymatic processes in life and is of paramount importance for many applications such as potential prototypes of new sensory devices, new extraction agents, and biological carriers. Surprisingly, synthetic receptors and sensors for anions are still very limited. The proposal will focus on developing new anion receptors, binders and sensors, which will have application in improving human health and the environment. The project is based on the hypothesis that the introduction of (C=S)NH, as a binding site to a synthetic receptor, enhances anion binding ability, and a fluorophore group if linked to the molecule displays a detectable optical change in presence of an anion. The long term goal of this project is to provide strategies for the design of highly selective sensor systems, and thus it will add a new dimension to the field of anion sensor chemistry. In Specific Aim 1, a new series of thioamide-based receptors with different spacers and variable rigidity will be synthesized, which include (1) acyclic thioamide receptors with TREN, aromatic and crown ether units, (ii) macrocyclic thioamide receptors with variable spacers, and (iii) optical sensors containing pyrene and dansyl groups. Series (i) and (ii) will bind anions through (C=S)NH...X- interactions, while the signaling unit(s) in series (iii) will serve as anion sensing. Specific Aim 2 is designed to investigate the binding properties of the synthesized molecules for anions in different solvents. The target anions are sulfate, phosphate, nitrate, perchlorate and halides, which are directly related to environment health and public health. Specific Aim 3 will focus on isolating complexes of anions bound to our synthetic ligands, and determining the structure of those complexes by X-ray crystallography. In Specific Aim 4, the cytotoxicity of thioamide-based ligands will be studied in both human liver carcinoma and human thyroid cancer cells using the MTT assay. Successful completion of this project will contribute greatly to the development of a framework for environmental and biomedical applications, and to the achievement the overall goals of RCMI at Jackson State University.