Canine Histocompatibility Typing

核心B：犬的组织相容性分型 犬组织相容性分型核心是为了满足两种犬研究的需要而设计的 本计划项目助学金中的项目1和2。核心人员由一名核心主管、一名工作人员组成 科学家和一名技术人员。该服务的主要作用是对犬的组织相容性进行分型 鉴定用于造血细胞移植的犬白细胞抗原(DLA)相合的窝胎对 选择具有所需组织相容类型的狗对进行繁殖。这些努力至关重要，以便 狗将作为人类造血细胞移植的翻译模型。在打字的时候 努力集中在两个最多态的I类和II类基因座，DLA-88和DRB1，额外的努力 将涉及开发另外3个多等位基因DLAI基因座的分型方法。这一核心还将 培养树突状细胞，建立表达DLA-I类基因的转基因K562细胞系 等位基因，并开发四聚体，用于次要抗原特异性T细胞的鉴定和表征。

CORE B: CANINE HISTOCOMPATIBILITY TYPING The Canine Histocompatibility Typing Core is designed to meet the needs of the two canine research Projects 1 and 2 on this Program Project Grant. The Core personnel consist of a Core Director, a Staff Scientist, and one technician. The primary role of the service consists of canine histocompatibility typing to identify Dog Leukocyte Antigen (DLA)-identical littermate pairs for hematopoietic cell transplantation and to select pairs of dogs with desired histocompatibility type for breeding. These efforts are critical in order for the dog to function as a translational model for human hematopoietic cell transplantation. While the typing effort is focused on the two most polymorphic class I and class II loci, DLA-88 and DRB1, additional efforts will involve developing methods for typing 3 additional polyallelic DLAclass I loci. This Core will also generate dendritic cells, develop genetically modified K562 cell lines for expression of selected DLA class I alleles, and develop tetramers for minor antigen-specific T cell identification and characterization.