Canine Histocompatibility Typing

犬组织相容性分型

• 批准号: 8459336
• 负责人: SCOTT Stoll GRAVES
• 金额: $ 11.02万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8459336
• 关键词: Alleles; Allogenic; Allografting; Amino Acids; Antigens; Bacteriophages; Base Sequence; Breeding; Canis familiaris; Cell Line; Cell membrane; Chimerism; Chromosomes; Clinical; Complementary DNA; Dendritic Cells; Exons; Frequencies; Gene Structure; Genes; Genome; Genomics; Genotype; HLA Antigens; Hematopoietic; Histocompatibility Antigens Class II; Histocompatibility Testing; Human; Human Resources; Immunoglobulin Variable Region; In Vitro; Introns; K562 Cells; Knowledge; Libraries; Location; MHC Class I Genes; MHC Class II Genes; Major Histocompatibility Complex; Mammals; Methods; Minor; Modeling; Molecular; Molecular Analysis; Molecular Probes; Molecular Structure; Mus; Names; Nucleotides; Organ Transplantation; Outcome; Pre-Clinical Model; Principal Investigator; Program Research Project Grants; Research Project Grants; Restriction fragment length polymorphism; Role; Scientist; Serological; Services; Solid; Stem cells; T-Lymphocyte; Techniques; United States National Institutes of Health; Variant; base; design; genome database; hematopoietic cell transplantation; human leukocyte antigen gene; meetings; programs; prospective; web site

CORE B: CANINE HISTOCOMPATIBILITY TYPING The Canine Histocompatibility Typing Core is designed to meet the needs of the two canine research Projects 1 and 2 on this Program Project Grant. The Core personnel consist of a Core Director, a Staff Scientist, and one technician. The primary role of the service consists of canine histocompatibility typing to identify Dog Leukocyte Antigen (DLA)-identical littermate pairs for hematopoietic cell transplantation and to select pairs of dogs with desired histocompatibility type for breeding. These efforts are critical in order for the dog to function as a translational model for human hematopoietic cell transplantation. While the typing effort is focused on the two most polymorphic class I and class II loci, DLA-88 and DRB1, additional efforts will involve developing methods for typing 3 additional polyallelic DLAclass I loci. This Core will also generate dendritic cells, develop genetically modified K562 cell lines for expression of selected DLA class I alleles, and develop tetramers for minor antigen-specific T cell identification and characterization.

核心b：犬组织相容性分型