Hopkins Digestive Diseases Basic and Translational Research Core Center

霍普金斯消化疾病基础和转化研究核心中心是由Silvio Conte提议的核心中心，旨在推进约翰霍普金斯大学医学院的基础科学和转化消化疾病研究。该中心的主题是“上皮功能的调控，特别是通过信号转导、运输和发育的变化”。它有一个由38个正式成员和12个准成员组成的研究基地。它由四个科学核心和一个行政核心组成。核心A，蛋白质组学核心将帮助识别在胃肠道/肝脏正常或疾病中差异表达的蛋白质，蛋白质之间的相互作用，包括鉴定结合伙伴，将绘制切割位点并确定翻译后修饰。Core B， Imaging Core将为核心中心的研究人员提供多种类型的前沿上皮细胞成像和EM（扫描、传输、免疫），并提供Core Manager来帮助进行研究。这些包括共聚焦和双光子显微镜与FRAP， FRET和TIRF；使用荧光计；单细胞定量成像；数码相机;访问激光捕获显微镜。核心C，小鼠生理学核心将为建立小鼠菌落提供建议；消化道疾病小鼠模型的基因分型；准备代谢笼，进行尿液、粪便、血液收集和分析。该核心还将进行小鼠的主动脉灌注，作为肠道、肝脏、胰腺、肾脏的一部分，并为北部和西部分析和组织学切片制备做准备。小鼠病理学家，细胞因子多重ELISA和FACS技术员是Core C的新组成部分。Core C提供设备，并提供使用说明，用于使用腔室/电压钳研究。核心D，即转化研究增强核心（Translational Research Enhancement Core），帮助建立临床数据库，收集患者的DNA、RNA样本，采购样本。行政核心（Administrative Core）将提供核心中心的沟通、财务管理和行政职能，组织充实计划（Enrichment Program），管理试点项目（Pilot Project）和迷你休假计划（mini - sabatical Programs）。

The Hopkins Digestive Diseases Basic and Translational Research Core Center is a proposed Silvio Conte Core Center which has been designed to advance basic science and translational digestive diseases research at the Johns Hopkins University School of Medicine. This Core Center has as its theme, "Regulation of Epithelial Function, Especially via Changes in Signal Transduction, Trafficking and Development". It has a Research Base of 38 full Members and 12 Associate Members. It consists of: 4 scientific Cores and an Administrative Core. Core A, Proteomics Core will help identify proteins that are differentially expressed in GI tract/liver normally or in diseases, interactions among proteins including identification of binding partners, will map cleavage sites and determine post-translational modifications. Core B, Imaging Core will make multiple types of cutting edge imaging and EM (scanning, transmission, immuno) of epithelial cells available to Core Center investigators and provide a Core Manager to help perform the studies. These include confocal and two-photon microscopy with FRAP, FRET and TIRF; use of fluorometers; single cell quantitative imaging; digital camera; access to Laser Capture Microscopy. Core C, Mouse Physiology Core will provide advice in establishing mouse colonies; genotype mouse models of digestive diseases; make available metabolic cages and perform urine, stool, blood collections and analysis. This Core will also perform aortic perfusion of mice as part of intestinal, liver pancreas, kidney procurement and preparation for Northern and Western analysis and histological slide preparation. A mouse pathologist, cytokine multiplex ELISA and a FACS technician are new components of Core C. Core C makes equipment available and provides instruction in its use for Ussing chamber/voltage clamp studies. Core D, Translational Research Enhancement Core provides help in forming a clinical database and specimen collection from patients for DNA, RNA, specimen procurement Administrative Core will provide the communication, financial management and administrative functions of the Core Center, organize the Enrichment Program and administer the Pilot Project and Mini-sabbatical Programs.