Hopkins Digestive Diseases Basic and Translational Research Core Center

霍普金斯消化疾病基础与转化研究核心中心

• 批准号: 8893061
• 负责人: MARK DONOWITZ
• 金额: $ 121.66万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8893061
• 关键词: A Mouse; Basic Science; Binding; Blood; Cells; Clinical; Collection; Communication; DNA; Databases; Development; Digestive System Disorders; Disease; Enzyme-Linked Immunosorbent Assay; Epithelial; Epithelial Cells; Equipment; Feces; Fluorescence Resonance Energy Transfer; Gastrointestinal tract structure; Genotype; Health; Image; Instruction; Intestines; Kidney; Lasers; Liver; Maps; Metabolic; Microscopy; Mus; Occupational activity of managing finances; Pancreas; Pathologist; Patients; Perfusion; Physiology; Pilot Projects; Post-Translational Protein Processing; Preparation; Proteins; Proteomics; RNA; Regulation; Research; Research Personnel; Scanning; Signal Transduction; Site; Slide; Specimen; Tissues; Translational Research; Universities; Urine; base; cytokine; design; differential expression; digital; medical schools; member; mouse model; programs; quantitative imaging; sample collection; trafficking; transmission process; two-photon; voltage clamp

The Hopkins Digestive Diseases Basic and Translational Research Core Center is a proposed Silvio Conte Core Center which has been designed to advance basic science and translational digestive diseases research at the Johns Hopkins University School of Medicine. This Core Center has as its theme, "Regulation of Epithelial Function, Especially via Changes in Signal Transduction, Trafficking and Development". It has a Research Base of 38 full Members and 12 Associate Members. It consists of: 4 scientific Cores and an Administrative Core. Core A, Proteomics Core will help identify proteins that are differentially expressed in GI tract/liver normally or in diseases, interactions among proteins including identification of binding partners, will map cleavage sites and determine post-translational modifications. Core B, Imaging Core will make multiple types of cutting edge imaging and EM (scanning, transmission, immuno) of epithelial cells available to Core Center investigators and provide a Core Manager to help perform the studies. These include confocal and two-photon microscopy with FRAP, FRET and TIRF; use of fluorometers; single cell quantitative imaging; digital camera; access to Laser Capture Microscopy. Core C, Mouse Physiology Core will provide advice in establishing mouse colonies; genotype mouse models of digestive diseases; make available metabolic cages and perform urine, stool, blood collections and analysis. This Core will also perform aortic perfusion of mice as part of intestinal, liver pancreas, kidney procurement and preparation for Northern and Western analysis and histological slide preparation. A mouse pathologist, cytokine multiplex ELISA and a FACS technician are new components of Core C. Core C makes equipment available and provides instruction in its use for Ussing chamber/voltage clamp studies. Core D, Translational Research Enhancement Core provides help in forming a clinical database and specimen collection from patients for DNA, RNA, specimen procurement Administrative Core will provide the communication, financial management and administrative functions of the Core Center, organize the Enrichment Program and administer the Pilot Project and Mini-sabbatical Programs.

霍普金斯消化疾病基础和转化研究核心中心是一个拟议的西尔维奥·孔蒂核心中心，旨在推进约翰霍普金斯大学医学院的基础科学和转化消化疾病研究。该中心的主题是“调节上皮功能，特别是通过信号转导，运输和发育的变化”。它有38个正式成员和12个准成员的研究基地。它由四个科学核心和一个行政核心组成。核心A，蛋白质组学核心将有助于识别在胃肠道/肝脏正常或疾病中差异表达的蛋白质，蛋白质之间的相互作用，包括识别结合伴侣，将绘制切割位点并确定翻译后修饰。Core B，Imaging Core将为Core Center研究者提供多种类型的尖端成像和上皮细胞EM（扫描、透射、免疫），并提供一名Core Manager帮助进行研究。这些包括与FRAP，FRET和TIRF共聚焦和双光子显微镜;使用荧光计;单细胞定量成像;数码相机;获得激光捕获显微镜。核心C，小鼠生理学核心将提供建立小鼠群体的建议;消化系统疾病的基因型小鼠模型;提供代谢笼，并进行尿液，粪便，血液收集和分析。该核心还将进行小鼠的主动脉灌注，作为肠道、肝脏、胰腺、肾脏采集和北方和西方分析准备以及组织学载玻片制备的一部分。小鼠病理学家、细胞因子多重ELISA和FACS技术员是核心C的新组成部分。核心C使设备可用，并提供其用于Ussing室/电压钳研究的说明。核心D，转化研究增强核心提供帮助，形成一个临床数据库和标本收集从病人的DNA，RNA，标本采购行政核心将提供沟通，财务管理和核心中心的行政职能，组织丰富计划和管理试点项目和迷你休假计划。