LOUISIANA COBRE: OBESITY & DIABETES RES: CELL BIOLOGY & BIOIMAGING CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 细胞生物学和生物成像核心(http://labs.pbrc.edu/cellbiology/index.htm))是为了满足科布雷项目绩效指标和彭宁顿生物医学研究中心研究人员的特定需求而组织的。它的目的是开发和提供最先进的生物成像方法，利用Micro CT研究活的和固定的细胞和组织中的生物过程的细胞和分子生物学，以及体内的生理过程。CBB核心提供最先进的生物成像和分析仪器和支持，以促进Cobre Project PI和PBRC教职员工的数据收集和分析。目前，CBBC的工作人员是担任主任的David H.Burk博士、担任副主任的Barbara Kozak博士以及两名研究助理Courtney Cain女士和Drury Ingram先生。 Pennington Cobre于2006年9月获得资助，成立了细胞生物学和生物成像核心，并围绕五个主要分析平台进行组织：蔡司LSM-510准多光子共焦、直立式荧光显微镜和倒置荧光显微镜、用于活细胞成像的扫描荧光仪和FACS口径分析细胞分选器。头两年的主要重点是加强我们现有的显微镜专业知识，并在流式细胞仪、免疫组织化学、成像和图像分析领域开发新的专业知识。我们的总体目标是通过提供对这些技术的无缝访问来增强我们教师的竞争力。 CBBC大致分为成像、组织学和标本制备和流式细胞术三个部分。在成像部分，核心有两个多光子共焦装置，一个用于高速比率成像的全电动倒置系统，一个用于固定细胞或组织成像的直立式电动系统，一个多色激光TIRF装置，两个用于常规检查载玻片或多孔板(亮场和荧光)的手动显微镜，以及一个数字病理载玻片扫描系统。组织学和标本制备部拥有四台冷冻机、三台旋转显微切割机、一台自动组织处理机、组织包埋站、自动玻片染色机/盖拖鞋、自动IHC染色机和一台激光显微切割系统。Core的流式细胞仪部分配备了用于四色分析的BD FACSCalibur和配备在BSL2层流罩内的iCyt反射高速细胞分选机。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell Biology and Bioimaging (CBB) Core (http://labs.pbrc.edu/cellbiology/index.htm) was organized in response to the specific needs of COBRE Project PIs and researchers at the Pennington Biomedical Research Center. Its purpose is to develop and provide state of the art bioimaging approaches to study the cell and molecular biology of biological processes in live and fixed cells and tissues, as well as physiological processes in vivo using micro CT. The CBB Core provides state of the art bioimaging and analytical instrumentation and support to facilitate data collection and analysis by COBRE Project PIs and PBRC faculty. Currently the CBBC is staffed by Dr. David H. Burk who serves as director, Dr. Barbara Kozak who serves as associate director and two research associates Ms. Courtney Cain and Mr. Drury Ingram. Funding of the Pennington COBRE in September 2006 brought the Cell Biology and Bioimaging Core into existence and it was organized around five major analytical platforms: a Zeiss LSM-510 meta multiphoton confocal, an upright epifluorescence microscope and an inverted epifluorescence microscope, a scanning fluorometer for live cell imaging, and a FACS Caliber analytical cell sorter. A primary focus in the first two years was to enhance our existing microscopy expertise and development new expertise in the areas of flow cytometry, immunohistochemistry, imaging and image analysis. Our overall goal is to enhance the competitiveness of our faculty by providing seamless access to these technologies. The CBBC is loosely divided into three sections  imaging, histology & specimen preparation, and flow cytometry. Within the imaging section, the core has two multi-photon confocals, one fully motorized inverted system for high speed ratiometric imaging, an upright motorized system for fixed cell or tissue imaging, a multi-color laser TIRF rig, two manual scopes for routine examination of slides or multi-well plates (brightfield and fluorescence), and a digital pathology slide scanning system. The histology & specimen preparation section holds four cryostats, three rotary microtomes, an automated tissue processor, tissue embedding station, automated slide stainer/coverslipper, auto IHC stainer, and a laser microdissection system. The Core's flow cytometry section houses a BD FACSCalibur for 4 color analysis and an iCyt Reflection high speed cell sorter equipped within a BSL2 lamellar flow hood.