CORE B: PROGENITOR CELL ANALYSIS CORE

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 对于该核心，一个主要功能是提供高水平的流式细胞术功能，以根据免疫学、遗传、物理和/或代谢特性分析干细胞和祖细胞群（及其后代）。这些功能对于处理罕见的多能干细胞和祖细胞群非常重要，而且在整个细胞和生物医学研究中也有出色的应用。 Core B 特别配备了 Becton Dickinson FACS-Calibur 流式细胞仪，可进行 4 色（免疫）染色以及前向和侧向散射分析。它还与配备高端软件的专用工作站相结合，用于单细胞水平的群体分析（包括细胞凋亡和细胞周期参数）。还支持 Flo-Jo、Win-List 和相关程序，以促进复杂细胞图的出版质量图形（通过专用工作站）。 随着我们中心（和研究所）的不断成功，我们的流式细胞术系统的使用量显着增加。 在今年的第 7 年，用户也开始更多地使用该 Core 的多孔高通量荧光测定/发光测定仪器（带有新获得的模量系统和注射器）以及多功能 Vi-Cell 细胞分析仪。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For this Core, one prime function is to provide high level flow cytometric capabilities to analyze stem and progenitor cell populations (and their progeny) based on immunological, genetic, physical, and/or metabolic properties. These capabilities are of importance in working with rare pluripotent stem and progenitor populations, but also find excellent application in cell and biomedical investigations at-large. Core B specifically is equipped with a Becton Dickinson FACS-Calibur flow cytometer that enables 4-color (immuno)staining plus forward and side scatter analyses. It's also coupled to dedicated workstations with high-end software for population analyses at the single-cell level (including apoptosis and cell cycle parameters). Flo-Jo, Win-List and related programs are also supported to facilitate publication-quality graphics of complex cytograms (via dedicated workstations). With the continuing success of our Center (and Institute), use of our flow cytometry system is increasing significantly. During present year-7, users also have begun to make increasing use of this Core's multi-well high-throughput fluorimetry / luminometry instrumentation (with a newly acquired modulus system plus injectors), as well as a versatile Vi-Cell cell analyzer.