CORE B: PROGENITOR CELL ANALYSIS CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 对于这一核心，一个主要功能是提供高水平的流式细胞术能力，根据免疫学、遗传学、物理和/或代谢特性分析干细胞和祖细胞群体(及其后代)。这些能力在处理稀有的多能干细胞和祖细胞种群方面很重要，但在细胞和生物医学研究中也有很好的应用。核心B特别配备了Becton Dickinson FACS-Calibur流式细胞仪，可以进行四色(免疫)染色以及正向和侧向散射分析。它还与配备高端软件的专用工作站相连，用于单细胞水平的群体分析(包括细胞凋亡和细胞周期参数)。还支持Flo-Jo、Win-List和相关程序，以促进复杂细胞图的出版质量图形(通过专用工作站)。随着我们中心(和研究所)的持续成功，我们的流式细胞仪系统的使用正在显著增加。在今年的第七年，用户也开始越来越多地使用这一核心的多井高通量荧光/发光测量仪器(带有新获得的模数系统和注射器)，以及多功能Vi-Cell细胞分析仪。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For this Core, one prime function is to provide high level flow cytometric capabilities to analyze stem and progenitor cell populations (and their progeny) based on immunological, genetic, physical, and/or metabolic properties. These capabilities are of importance in working with rare pluripotent stem and progenitor populations, but also find excellent application in cell and biomedical investigations at-large. Core B specifically is equipped with a Becton Dickinson FACS-Calibur flow cytometer that enables 4-color (immuno)staining plus forward and side scatter analyses. It's also coupled to dedicated workstations with high-end software for population analyses at the single-cell level (including apoptosis and cell cycle parameters). Flo-Jo, Win-List and related programs are also supported to facilitate publication-quality graphics of complex cytograms (via dedicated workstations). With the continuing success of our Center (and Institute), use of our flow cytometry system is increasing significantly. During present year-7, users also have begun to make increasing use of this Core's multi-well high-throughput fluorimetry / luminometry instrumentation (with a newly acquired modulus system plus injectors), as well as a versatile Vi-Cell cell analyzer.