CORE B: PROGENITOR CELL ANALYSIS CORE

核心 B：祖细胞分析核心

• 批准号: 8167685
• 负责人: Pradeep Sathyanarayana
• 金额: $ 8.7万
• 依托单位: MAINEHEALTH
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167685
• 关键词: Apoptosis; Cell Cycle; Cells; Color; Complex; Computer Retrieval of Information on Scientific Projects Database; Computer software; Coupled; Flow Cytometry; Funding; Genetic; Grant; Institutes; Institution; Investigation; Metabolic; Population; Property; Publications; Research; Research Personnel; Resources; Side; Source; Staining method; Stains; Stem cells; System; United States National Institutes of Health; Work; base; cell analyzer; instrumentation; progenitor; programs; stem; success

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For this Core, one prime function is to provide high level flow cytometric capabilities to analyze stem and progenitor cell populations (and their progeny) based on immunological, genetic, physical, and/or metabolic properties. These capabilities are of importance in working with rare pluripotent stem and progenitor populations, but also find excellent application in cell and biomedical investigations at-large. Core B specifically is equipped with a Becton Dickinson FACS-Calibur flow cytometer that enables 4-color (immuno)staining plus forward and side scatter analyses. It's also coupled to dedicated workstations with high-end software for population analyses at the single-cell level (including apoptosis and cell cycle parameters). Flo-Jo, Win-List and related programs are also supported to facilitate publication-quality graphics of complex cytograms (via dedicated workstations). With the continuing success of our Center (and Institute), use of our flow cytometry system is increasing significantly. During present year-7, users also have begun to make increasing use of this Core's multi-well high-throughput fluorimetry / luminometry instrumentation (with a newly acquired modulus system plus injectors), as well as a versatile Vi-Cell cell analyzer.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 对于该核心，一个主要功能是提供高水平的流式细胞术能力，以基于免疫学、遗传学、物理和/或代谢特性分析干细胞和祖细胞群（及其后代）。这些能力在与罕见的多能干细胞和祖细胞群体的工作中非常重要，而且在细胞和生物医学研究中也有很好的应用。 核心B专门配备了Becton Dickinson FACS-Calibur流式细胞仪，可进行四色（免疫）染色以及前向和侧向散射分析。它还与专用工作站耦合，该工作站配备高端软件，用于单细胞水平的群体分析（包括细胞凋亡和细胞周期参数）。还支持Flo-Jo、Win-List和相关程序，以促进复杂细胞图的出版质量图形（通过专用工作站）。 随着我们中心（和研究所）的持续成功，我们的流式细胞仪系统的使用显着增加。 在今年7月，用户也开始越来越多地使用该核心的多孔高通量荧光/光度仪器（与新收购的模数系统加注射器），以及一个多功能的Vi-Cell细胞分析仪。