CORE B: STEM & PROGENITOR CELL SEPARATION & ANALYSIS CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 对于这一核心，一个主要功能是提供最先进的能力，根据免疫、遗传、物理和/或代谢特性分离和分析祖细胞群体。这些能力在处理稀有的干细胞和祖细胞群体时特别重要，而且在细胞和生物医学研究中也有很好的应用。该核心现在配备了用于分离(或耗尽)和分析此类细胞类型的专用仪器。这包括Becton Dickinson FACS-Calibur流式细胞仪、新的FACS-Aria细胞分选器和Miltenyi Macs系统(自动磁性细胞分选器)(每个都通过研究所匹配的基金机制购买)。更多的用户是研究所范围内的，包括MMCRI血管生物学中心的研究人员。 正如最近美国国立卫生研究院研究部分的评论所强调的那样，核心B是我们干细胞科布雷中心的一个极其重要和高生产力的组成部分。它也是我们成功招聘工作的重要工具。 最近，核心B开发了一种平行资源，用于涉及胚胎干细胞(ES)使用的研究。特别是，为胚胎干细胞研究提供某些服务、技术支持和实践培训。特定的小鼠胚胎干细胞和美国国立卫生研究院批准的人类胚胎干细胞系在适当的质量保证下得到维护。支持还包括在胚胎干细胞维持、转导和分化方面的指导。这一核心组成部分还为个人研究人员提供短期培养和操纵胚胎干细胞的服务，以及随时获得胚胎干细胞试剂(RNA、基因组DNA、蛋白质制剂)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For this Core, one prime function is to provide state-of-the-art capabilities for the isolation and analysis of progenitor cell populations based on immunological, genetic, physical and/or metabolic properties. These capabilities are of particular importance in working with rare stem and progenitor cell populations, but also find excellent application in cell and biomedical investigations at-large. This Core is now equipped with dedicated instruments for the isolation (or depletion) and analysis of such cell types. This includes a Becton Dickinson FACS-Calibur flow cytometer, a new FACS-Aria cell sorter, and Miltenyi MACS System (Automated Magnetic Cell Sorter) (with each purchased via Institute matching fund mechanisms). Additional users are Institute- wide, including investigators in MMCRI's Vascular Biology COBRE. As emphasized in recent NIH study section critiques, Core B is a critically important and highly productive component of our Stem Cell COBRE Center. It also has been an important tool for our successful recruiting efforts. Recently, Core B has developed a parallel resource for investigations involving the use of embryonic stem (ES) cells. In particular, certain services, technical support, and hands-on training for ES cell research are provided. Specified Collections of mouse ES cells and NIH-approved human ES cell lines are maintained with appropriate quality assurances. Support further includes instruction in ES cell maintenance, transduction, and differentiation. This Core component also provides a service of short-term culture and manipulation of ES cells for individual investigators, plus ready access to ES cell reagents (RNA, genomic DNA, protein preparations).