CORE B: STEM & PROGENITOR CELL SEPARATION & ANALYSIS CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 对于该核心，一个主要功能是提供基于免疫学、遗传学、物理和/或代谢特性的祖细胞群的分离和分析的最新能力。 这些能力在处理罕见的干细胞和祖细胞群体时特别重要，而且在细胞和生物医学研究中也有很好的应用。该核心现在配备了专用仪器，用于分离（或消除）和分析此类细胞类型。这包括一台Becton Dickinson FACS-Calibur流式细胞仪、一台新的FACS-Aria细胞分选仪和Miltenyi MACS系统（自动磁性细胞分选仪）（均通过研究所匹配基金机制购买）。 其他用户是研究所范围内的，包括MMCRI的血管生物学COBRE的研究人员. 正如在最近的NIH研究部分评论中所强调的那样，核心B是我们干细胞COBRE中心的一个至关重要和高产的组成部分。 它也是我们成功招聘的重要工具。 最近，核心B已经开发了一个平行的资源，涉及使用胚胎干细胞（ES）的调查。 特别是，为ES细胞研究提供某些服务，技术支持和实践培训。 小鼠ES细胞和NIH批准的人ES细胞系的特定集合以适当的质量保证进行维护。 支持还包括指令在胚胎干细胞的维护，转导和分化。 该核心组件还为个体研究者提供ES细胞的短期培养和操作服务，以及随时获得ES细胞试剂（RNA，基因组DNA，蛋白质制剂）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For this Core, one prime function is to provide state-of-the-art capabilities for the isolation and analysis of progenitor cell populations based on immunological, genetic, physical and/or metabolic properties. These capabilities are of particular importance in working with rare stem and progenitor cell populations, but also find excellent application in cell and biomedical investigations at-large. This Core is now equipped with dedicated instruments for the isolation (or depletion) and analysis of such cell types. This includes a Becton Dickinson FACS-Calibur flow cytometer, a new FACS-Aria cell sorter, and Miltenyi MACS System (Automated Magnetic Cell Sorter) (with each purchased via Institute matching fund mechanisms). Additional users are Institute- wide, including investigators in MMCRI's Vascular Biology COBRE. As emphasized in recent NIH study section critiques, Core B is a critically important and highly productive component of our Stem Cell COBRE Center. It also has been an important tool for our successful recruiting efforts. Recently, Core B has developed a parallel resource for investigations involving the use of embryonic stem (ES) cells. In particular, certain services, technical support, and hands-on training for ES cell research are provided. Specified Collections of mouse ES cells and NIH-approved human ES cell lines are maintained with appropriate quality assurances. Support further includes instruction in ES cell maintenance, transduction, and differentiation. This Core component also provides a service of short-term culture and manipulation of ES cells for individual investigators, plus ready access to ES cell reagents (RNA, genomic DNA, protein preparations).