CORE B: STEM & PROGENITOR CELL SEPARATION & ANALYSIS CORE

核心B：STEM

• 批准号: 7720702
• 负责人: Pradeep Sathyanarayana
• 金额: $ 11.76万
• 依托单位: MAINEHEALTH
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-04 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720702
• 关键词: Arts; Biology; Blood Vessels; Cell Maintenance; Cell Separation; Cells; Centers of Research Excellence; Collection; Computer Retrieval of Information on Scientific Projects Database; Critiques; DNA; Funding; Funding Mechanisms; Genetic; Genomics; Grant; Hand; Individual; Institutes; Institution; Instruction; Investigation; Magnetism; Metabolic; Mus; Population; Preparation; Property; Proteins; Reagent; Recruitment Activity; Research; Research Personnel; Resources; Services; Source; Specific qualifier value; Stem Cell Research; Stem cells; Study Section; System; Training; United States National Institutes of Health; Work; base; cell type; embryonic stem cell; human embryonic stem cell line; instrument; quality assurance; stem; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For this Core, one prime function is to provide state-of-the-art capabilities for the isolation and analysis of progenitor cell populations based on immunological, genetic, physical and/or metabolic properties. These capabilities are of particular importance in working with rare stem and progenitor cell populations, but also find excellent application in cell and biomedical investigations at-large. This Core is now equipped with dedicated instruments for the isolation (or depletion) and analysis of such cell types. This includes a Becton Dickinson FACS-Calibur flow cytometer, a new FACS-Aria cell sorter, and Miltenyi MACS System (Automated Magnetic Cell Sorter) (with each purchased via Institute matching fund mechanisms). Additional users are Institute- wide, including investigators in MMCRI's Vascular Biology COBRE. As emphasized in recent NIH study section critiques, Core B is a critically important and highly productive component of our Stem Cell COBRE Center. It also has been an important tool for our successful recruiting efforts. Recently, Core B has developed a parallel resource for investigations involving the use of embryonic stem (ES) cells. In particular, certain services, technical support, and hands-on training for ES cell research are provided. Specified Collections of mouse ES cells and NIH-approved human ES cell lines are maintained with appropriate quality assurances. Support further includes instruction in ES cell maintenance, transduction, and differentiation. This Core component also provides a service of short-term culture and manipulation of ES cells for individual investigators, plus ready access to ES cell reagents (RNA, genomic DNA, protein preparations).

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 对于该核心，一个主要功能是根据免疫学、遗传、物理和/或代谢特性提供最先进的祖细胞群分离和分析能力。 这些功能在处理稀有干细胞和祖细胞群时特别重要，而且在整个细胞和生物医学研究中也有出色的应用。该核心现在配备了用于分离（或去除）和分析此类细胞类型的专用仪器。其中包括 Becton Dickinson FACS-Calibur 流式细胞仪、新型 FACS-Aria 细胞分选仪和 Miltenyi MACS 系统（自动磁性细胞分选仪）（均通过研究所配套资金机制购买）。 其他用户是研究所范围内的，包括 MMCRI 血管生物学 COBRE 的研究人员。 正如最近 NIH 研究部门批评中所强调的那样，Core B 是我们干细胞 COBRE 中心极其重要且高效的组成部分。 它也是我们成功招聘的重要工具。 最近，Core B 开发了一种并行资源，用于涉及胚胎干 (ES) 细胞使用的研究。 特别是为胚胎干细胞研究提供某些服务、技术支持和实践培训。 小鼠 ES 细胞和 NIH 批准的人类 ES 细胞系的指定集合均得到适当的质量保证。 支持还包括 ES 细胞维持、转导和分化方面的指导。 该核心组件还为个人研究人员提供 ES 细胞的短期培养和操作服务，以及随时获取 ES 细胞试剂（RNA、基因组 DNA、蛋白质制剂）的服务。