Progenitor Cell Analysis Core

祖细胞分析核心

• 批准号: 8543894
• 负责人: Pradeep Sathyanarayana
• 金额: $ 9.22万
• 依托单位: MAINEHEALTH
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8543894
• 关键词: Agreement; Algorithms; Apoptosis; Applications Grants; Area; Biological Assay; Biomedical Research; Carbon Dioxide; Cell Culture System; Cell Culture Techniques; Cell Cycle; Cell Line; Cell Separation; Cell surface; Cells; Cellular biology; Centers of Research Excellence; Clinical; Collection; Color; Complex; Confocal Microscopy; Consultations; DNA; Data; Development; Electroporation; Faculty; Flow Cytometry; Genomics; Histopathology; Hypoxia; Incubators; Injectable; Institutes; Instruction; Investigation; Laboratories; Lasers; Licensing; Magnetism; Microscopy; Mus; Phase; Phosphoproteins; Physiology; Population; Preparation; Property; Proteins; Quantitative Microscopy; RNA; Reagent; Regenerative Medicine; Research; Research Personnel; Resources; Role; Scanning; Services; Signal Transduction; Stem cells; System; Training; Transfection; United States National Institutes of Health; Viral Vector; base; cell preparation; cellular imaging; embryonic stem cell; fluorescence microscope; human embryonic stem cell line; induced pluripotent stem cell; innovation; instrumentation; medical specialties; molecular phenotype; quality assurance; release of sequestered calcium ion into cytoplasm; stem; tissue culture; vector

PROJECT SUMMARY (See Instructions): Towards advancing Center and Institute investigations (and continued Center development), our Progenitor Cell Analysis Core provides expert services, training, instrumentation and resources in three related areas: i) analytical flow cytometry, together with FACS and MACS cell isolation services; ii) high through-put colony/cell imaging, fluorimetry, and luminometry; iii) services/resources for stem & progenitor cell culture, including unique cell populations, plus ES cells and approved cell lines. Several core supported services (eg, flow cytometry and FACS) are indispensable, unique components, and are used widely by Center and Institute investigators. Combined instrumentation as a BD FacsCalibur flow cytometer plus FACSAria-2 cell sorter (near-UV laser) enable complex four color multi-parameter analyses. This includes cell surface markers, as well as (co-) analyses of cell cycle parameters, apoptosis, phosphoprotein signaling, ROS, calcium flux and a number of additional informative cell properties. Our expert staff are also skilled in the enrichment and isolation of rare cell sub-populations (eg, using combined MACS plus FACS approaches). In addition, this core supports basic analyses (and manipulations of) progenitor cell populations (Vicell system, injectable multi-well luminometer, fluorimeter, electroporation/transfection units). Recently, we also have launched several innovative cell-based analyses, with cell and colony scanning via STEMvision as one example. With regards to services and resources for the culture and manipulation of stem and progenitor cells, these benefit further from a dedicated tissue culture facility (Zeiss and Evos fluorescent microscopy systems, cell culture hoods and incubators, hypoxia chambers, clinical centrifuges, specialty reagents). Our Progenitor Cell Analysis Core also interacts directly with Center and Institute cores in Histopathology, Molecular Phenotyping, Confocal Microscopy and Viral Vectors; facilitates productive multi-unit interactions; provides vital roles in new Center and Institute faculty recruitment; and continues to advance our biomedical research investigations (together with nationally competitive grant applications).

项目摘要（见说明）：推进中心和研究所的调查（和持续的中心发展），我们的祖细胞分析核心在三个相关领域提供专家服务，培训，仪器和资源：i）分析流式细胞术，以及FACS和MACS细胞分离服务; ii）高通量集落/细胞成像，荧光测定和发光测定; iii）干细胞和祖细胞培养的服务/资源，包括独特的细胞群，加上ES细胞和批准的细胞系。几项核心支持服务（如流式细胞术和流式细胞仪）是不可或缺的独特组成部分，并被中心和研究所研究者广泛使用。BD FacsCalibur流式细胞仪和FACSAria-2细胞分选仪（近紫外激光）的组合仪器能够进行复杂的四色多参数分析。这包括细胞表面标志物，以及细胞周期参数、细胞凋亡、磷蛋白信号传导、ROS、钙通量和许多其他信息性细胞特性的（共同）分析。我们的专家工作人员还擅长稀有细胞亚群的富集和分离（例如，使用组合的MACS加FACS方法）。此外，该核心支持祖细胞群的基本分析（和操作）（Vicell系统、可注射多孔光度计、荧光计、电穿孔/转染装置）。最近，我们还推出了几项创新的基于细胞的分析，例如通过STEMvision进行细胞和菌落扫描。关于干细胞和祖细胞培养和操作的服务和资源，这些进一步受益于专用的组织培养设施（Zeiss和Evos荧光显微镜系统、细胞培养罩和培养箱、低氧室、临床离心机、专用试剂）。我们的祖细胞分析核心还与组织学，分子表型分析，共聚焦显微镜和病毒载体的中心和研究所核心直接互动;促进生产性的多单元互动;在新的中心和研究所教师招聘中发挥重要作用;并继续推进我们的生物医学研究调查（以及具有国家竞争力的拨款申请）。