CORE B: PROGENITOR CELL ANALYSIS CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 对于这一核心，一个主要功能是提供高水平的流式细胞术能力，以基于免疫学、遗传学、物理和/或代谢特性分析祖细胞群体和后代。这些能力在处理稀有的干细胞和祖细胞种群方面很重要，但在细胞和生物医学研究中也有很好的应用。具体地说，这个核心配备了Becton Dickinson FACS-Calibur流式细胞仪，可以进行四色(免疫)染色以及正向和侧向散射分析。它还与配备高端软件的专用工作站相连，用于单细胞水平的种群分析，包括细胞周期参数。还支持Flo-Jo、Win-List和相关程序，以促进完整细胞图谱的出版质量图形。 随着我们中心(和研究所)的持续成功，我们的流式细胞仪系统的高效使用显着增加(以至于我们最近申请了缅因州研发债券基金来购买第二个BD系统)。对于核心B的这一方面，并允许ES细胞以及FACS和MACS组件在强大的初始支持下并行发展，后一套设施和服务(ES细胞、FACS、MACS)已在第6年(通过NCRR批准)重新构建为独立的核心E。这也使核心B开始开发多井高通量荧光/发光测量(新获得的模数系统加上注射器)，并高效地使用多功能Vi-cell分析仪。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For this Core, one prime function is to provide high level flow cytometric capabilities to analyze progenitor cell populations and progeny based on immunological, genetic, physical, and/or metabolic properties. These capabilities are of importance in working with rare stem and progenitor populations, but also find excellent application in cell and biomedical investigations at-large. Specifically, this Core is equipped with a Becton Dickinson FACS-Calibur flow cytometer that enables 4-color (immuno)staining plus forward and side scatter analyses. It's also coupled to dedicated workstations with high-end software for population analyses at the single-cell level, including cell cycle parameters. Flo-Jo, Win-List and related programs are also supported to facilitate publication-quality graphics of complete cytograms. With the continuing success of our Center (and Institute) highly productive use of our flow cytometry system has increased markedly (to the extent that we've recently applied for Maine state R & D bond funds to purchase a second BD system). For this aspect of Core B, and to allow ES cell as well as FACS and MACS components to develop in parallel with strong initial support, this latter set of facilities and services (ES cell, FACS, MACS) have been re-framed in year-6 (via NCRR approval) as separate-standing Core E. This also has allowed Core B to begin to develop multi-well high-throughput fluorimetry / luminometry (newly acquired modulus system plus injectors) and to make efficient day-to-day use of a versatile Vi-Cell cell analyzer.