LSU VETERINARY COBRE: PATHOGENESIS OF RICKETTSIA SP

路易斯安那州立大学兽医 COBRE：立克次体 SP 的发病机制

• 批准号: 8167884
• 负责人: Kevin R. Macaluso
• 金额: $ 19.98万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167884
• 关键词: Adult; Antigens; Blood capillaries; Cellular Structures; Computer Retrieval of Information on Scientific Projects Database; Coupled; Cues; Dermacentor; Event; Funding; Gene Expression; Grant; Immunoblotting; Individual; Infection; Institution; Larva; Liquid Chromatography; Metabolic; Nymph; Ovary; Pathogenesis; Proteins; Research; Research Personnel; Resources; Rickettsia; Rickettsia rickettsii; Salivary Glands; Source; Staging; Ticks; Time; Tissues; Two-Dimensional Gel Electrophoresis; United States National Institutes of Health; Vertical Disease Transmission; capillary; egg; feeding; novel; polyclonal antibody; protein expression; protein profiling; transmission process; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Specific Aims. Aim 1. Characterize the rate at which SFG rickettsiae spread through individual ticks and throughout individual tick tissues. Uninfected Dermacentor variabilis will be exposed to Rickettsia rickettsii, R. montanensis, or R. bellii via capillary feeding. Parameters of SFG infection and transmission, including dispersion and multiplication, in individual tick tissues will be determined. A. Assess SFG rickettsial infection and subsequent tissue dissemination in the tick vector using quantitative real-time PCR. B. Quantify rickettsiae associated with horizontal (salivary gland) and vertical (ovary) transmission events in infected egg, larva, nymph, and adult stages. Aim 2. Identify and characterize uniquely expressed protein from three tick-borne rickettsiae in tick hosts during horizontal and vertical transmission events. Although not well understood, a metabolic union between other SFG rickettsiae and their tick hosts has been described. Rickettsia undergo a reactivation event changing in cell structure and vertebrate infectivity in association with host environmental cues coupled to bloodmeal acquisition. This aim will provide novel information concerning tick-borne rickettsiae in relation to global protein expression and antigenic profiles. A. Initial studies will compare protein profiles of SFG rickettsiae during transmission events within the tick tissues involved in horizontal (salivary gland) and vertical (ovary) transmission by two dimensional gel electrophoresis. B. Identify rickettsial antigens by immunoblot using polyclonal antibodies to tick-borne rickettsiae and liquid chromatography quadruple-time-of-flight (2DLC-QTOF) analysis. C. Compare observed protein expression to gene expression, as assessed by real-time PCR.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 具体目标。 AIM 1。表征SFG立克西亚通过单个壁虱和整个单个tick组织传播的速度。未感染的Dermacentor variabilis将通过毛细管喂养暴露于立克西亚立克西氏菌，R。Montanensis或R. Bellii。将确定单个tick组织中SFG感染和传播的参数，包括分散和乘法。 A.使用定量实时PCR评估tick矢量中的SFG人力素感染和随后的组织传播。 B.量化与水平（唾液腺）和感染卵，幼虫，若虫和成人阶段的水平（唾液腺）和垂直（卵巢）传播事件相关的人力体。 AIM 2。在水平和垂直传输事件期间，在tick宿主中的三个tick传播人力体中识别并表征唯一表达的蛋白质。尽管不太了解，但已经描述了其他SFG Rickettsiae及其tick宿主之间的代谢结合。人力车经历了细胞结构和脊椎动物感染性的重新激活事件，并与宿主环境提示相关联，并与血液采集结合。该目标将提供有关tick传播的立克西亚与全球蛋白质表达和抗原谱有关的新信息。 A.初步研究将通过二维凝胶电泳在涉及水平（唾液腺）和垂直（卵巢）透射的tick组织中的传输事件中的SFG立克赛的蛋白质曲线进行比较。 B.通过使用多克隆抗体来鉴定出tick传播的立克西亚和液相色谱Quadruple-Flipter（2DLC-QTOF）分析的双胞胎抗原。 C.通过实时PCR评估，将观察到的蛋白表达与基因表达进行比较。