Molecular Immunopathology Core

分子免疫病理学核心

基本信息

批准号：
8751067
负责人：
Kevin R. Macaluso
金额：
$ 15.43万
依托单位：
LOUISIANA STATE UNIV A&M COL BATON ROUGE
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-06-02 至 2019-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8751067
关键词：
Address Advertising Animal Model Animals Antibody Formation Appointment Arthropod Vectors Arthropods Bioinformatics Biological Assay Biopsy Cells Centers of Research Excellence Certification Collaborations Communicable Diseases Confocal Microscopy Core Facility Development Diagnosis Disease Ensure Equipment Faculty Fees Fiber Flow Cytometry Funding Future Goals Grant Human Resources Ice Image Immune Immunohistochemistry Immunology Immunophenotyping In Vitro Income Individual Infection Infectious Diseases Research Institution Joints Label Laboratories Laboratory Animal Medicine Lasers Lead Leadership Location Louisiana Methodology Microscope Microscopy Molecular Molecular Biology Nature Optics Participant Pathologist Pathology Phase Postdoctoral Fellow Primates Process Proteins Protocols documentation Reagent Research Research Infrastructure Research Personnel Research Support Resources Rodent Role Sampling Scanning Transmission Electron Microscopy Procedures Schedule Scientist Services Specialized Center Students Technology Testing Tissue Sample Tissues Training United States National Institutes of Health Veterinary Pathology Videoconferences Videoconferencing Viral Virus Work base cell type cost data exchange experience graduate student immunopathology instrument interest investigator training member new technology nonhuman primate novel pathogen pathogenic bacteria theories web site

项目摘要

During the previous two Phases ofthe COBRE "Center for Experimental Infectious Disease Research (CEIDR)", the CEIDR Molecular Immunopathology Core (MIPC) leveraged support of multiple Core Facilities and Equipment at both LSU and Tulane National Primate Research Center (TNPRC) to support NIH-funded infectious disease research. The original MIPC remains intact except that two specialized functions dealing with molecular biology, NGS and bioinformatics are now covered by the Genelab while protein and antibody production and protein characterization is covered by the PCL. MIPC now leverages and coordinates Core activities in flow cytometry, pathology and microscopy at both ISU-SVM and TNPRC. All three activities constitute established Core laboratories operating as a consortium of cost centers at both ISU-SVM and TNPRC. MIPC Units are highly likely to be sustained in the future, since currently, ISU-SVM provides up to 80% ofall personnel costs, while TNPC facilities are supported by the Primate Center NIH base grant and a robust fee-for-sen/ice income. LSU facilities focus primarily on rodents and arthropod vectors, while TNPRC facilities almost exclusively focus on non-human primate (NHP) research support. Thus, it is projected that COBRE phase 111 funding for these facilities will be easily replaced by fee-for-sen/ice income, ensuring a self-sustaining future for MIPC facilities. It is envisioned that increasing collaboration among LSU and TNPRC scientists, as evidenced by the presence of three collaborative projects between LSU-SVM and TNPRC scientists and a jointly hired faculty member (recruitment in process), will lead to enhancement of know-how and support functions for all MIPC facilities. Certain functions ofthe MIPC are unique to either location. The MIPC aims to 1) Provide access to specialized equipment and molecular immunopathological and microscopy expertise to CEIDR researchers. 2) Train investigators and their staff in the theory and use of technologies available in MIPC. 3) Promote the development and use of new technologies and assays that can be used to characterize pathogen-host interactions in vitro and in experimental animal infections (small animals and NHP).

在毛cor的前两个阶段，“实验性传染病研究中心（CEIDR）”，CEIDR分子免疫病理学核心（MIPC）杠杆的多个核心支持 LSU和Tulane国家灵长类动物研究中心（TNPRC）的设施和设备支持 NIH资助的传染病研究。原始MIPC保持完整，但两个专业涉及分子生物学，NGS和生物信息学的功能现在被Genelab涵盖，而蛋白质和抗体的产生和蛋白质表征由PCL覆盖。 MIPC现在利用并协调ISU-SVM和TNPRC流式细胞仪，病理和显微镜的核心活性。这三项活动构成了在两者的成本中心的财团中建立的核心实验室 ISU-SVM和TNPRC。 MIPC单元很可能将来维持，因为目前，ISU-SVM 提供多达80％的人员费用，而TNPC设施得到灵长类动物中心NIH的支持基础赠款和稳健的收费/冰收入。 LSU设施主要关注啮齿动物和节肢动物载体，而TNPRC设施几乎完全专注于非人类灵长类动物（NHP）研究支持。因此，可以预计，这些设施的Cobre第111阶段资金将很容易取代收费/冰收入，确保MIPC设施的自我维持的未来。可以预见的是增加 LSU和TNPRC科学家之间的合作，三个合作的存在证明了 LSU-SVM与TNPRC科学家以及共同聘用的教职员工之间的项目（招聘过程），将导致所有MIPC设施的专有技术和支持功能的增强。肯定 MIPC的功能在任何一个位置都是唯一的。 MIPC的目的是1）提供专业的访问权限对CEIDR研究人员的设备和分子免疫病理学和显微镜专业知识。 2）火车在MIPC中提供的技术和使用方面的研究人员及其员工。 3）促进开发和使用新技术和可用于表征病原体宿主的测定体外和实验动物感染（小动物和NHP）的相互作用。