ENHANCEMENT OF DENGUE VIRUS TRANSMISSION DUE TO SALIVARY PROTEINS OF ITS VECTOR

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 第1节登革热诱导埃及伊蚊唾液腺提取液差异蛋白表达 根据观察到的感染和未感染蚊体之间蛋白质表达的差异，我们决定评估这种现象是否也在它们的唾液腺中观察到。感染登革热病毒的唾液腺是登革热传播的必要步骤。 第二节登革病毒与唾液腺蛋白的相互作用 最近，几种来自伊蚊的唾液腺蛋白被发现与登革病毒结合(Cao-Lormeau等人。2009年)。然而，目前尚不清楚这些蛋白质是结构性的还是分泌性的。我们计划研究能够与登革热病毒四种血清型中的任何一种结合的唾液腺蛋白。 第三节.抗Ae的体液免疫反应埃及伊蚊 唾液腺蛋白已被证明是评估蚊子叮咬暴露的一个非常有用的工具。我们想要评估抗Ae抗体。登革热流行区人群埃及伊蚊SGE抗体水平。 第四节复杂登革热病毒生态系统的传播动态 在全球范围内，登革热病毒是最重要的虫媒病毒感染，影响着热带地区数百万人。我们正在研究在多种共同传播的血清型和毒株的背景下登革热病毒的传播动力学。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Section 1. Dengue-induced differential protein expression in salivary gland extracts of Aedes aegypti mosquitoes Based on the observed differences in protein expression between infected and uninfected mosquito bodies, we decided to evaluate if this phenomenon was also observed in their salivary glands. Infection of salivary glands by dengue virus is a required step in dengue fever transmission. Section 2. Interaction of Dengue virus with salivary gland proteins Recently, several salivary gland proteins from Aedes mosquitoes were found to bind to dengue virus (Cao-Lormeau et al. 2009). However, it was not clear whether these proteins were structural or secreted. We plan to study salivary gland proteins that are able to bind any of the four serotypes of dengue virus. Section 3. Humoral immune response against Ae. aegypti SGE Salivary gland proteins have shown to be a very useful tool for evaluating mosquito bite exposure. We wanted to evaluate the anti  Ae. aegypti SGE antibody level in people living in dengue endemic regions. Section 4. Transmission dynamics of complex dengue virus ecologies Globally, dengue virus is the most significant arboviral infection, affecting millions of people throughout the tropics. We are investigating the transmission dynamics of dengue virus in the context of multiple, co-circulating serotypes and strains.