ENHANCEMENT OF DENGUE VIRUS TRANSMISSION DUE TO SALIVARY PROTEINS OF ITS VECTOR

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 第1款.登革热诱导的埃及伊蚊唾液腺提取物差异蛋白表达 基于观察到的感染和未感染蚊子体内蛋白质表达的差异，我们决定评估这种现象是否也在它们的唾液腺中观察到。唾液腺被登革病毒感染是登革热传播的必要步骤。 第2款.登革病毒与唾液腺蛋白的相互作用 最近，发现来自伊蚊的几种唾液腺蛋白与登革热病毒结合（Cao-Lormeau等人，2009）。然而，尚不清楚这些蛋白质是结构性的还是分泌性的。我们计划研究唾液腺蛋白能够结合任何四种血清型的登革热病毒。 第3款.抗Ae的体液免疫应答埃及SGE 唾液腺蛋白已被证明是一个非常有用的工具，用于评估蚊虫叮咬暴露。我们想评估抗  AE.登革热流行区人群中埃及伊蚊SGE抗体水平。 第4款.复杂登革热病毒生态的传播动力学 在全球范围内，登革热病毒是最重要的虫媒病毒感染，影响了热带地区数百万人。我们正在调查登革热病毒在多种共循环血清型和毒株背景下的传播动力学。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Section 1. Dengue-induced differential protein expression in salivary gland extracts of Aedes aegypti mosquitoes Based on the observed differences in protein expression between infected and uninfected mosquito bodies, we decided to evaluate if this phenomenon was also observed in their salivary glands. Infection of salivary glands by dengue virus is a required step in dengue fever transmission. Section 2. Interaction of Dengue virus with salivary gland proteins Recently, several salivary gland proteins from Aedes mosquitoes were found to bind to dengue virus (Cao-Lormeau et al. 2009). However, it was not clear whether these proteins were structural or secreted. We plan to study salivary gland proteins that are able to bind any of the four serotypes of dengue virus. Section 3. Humoral immune response against Ae. aegypti SGE Salivary gland proteins have shown to be a very useful tool for evaluating mosquito bite exposure. We wanted to evaluate the anti  Ae. aegypti SGE antibody level in people living in dengue endemic regions. Section 4. Transmission dynamics of complex dengue virus ecologies Globally, dengue virus is the most significant arboviral infection, affecting millions of people throughout the tropics. We are investigating the transmission dynamics of dengue virus in the context of multiple, co-circulating serotypes and strains.