ANOPHELES SRPN6 FUNCTIONS DURING MALARIA PARASITE INVASION OF MOSQUITO EPITHELIA

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 尽管不断作出控制和预防努力，但疟疾仍然是世界范围内最具破坏性的传染病之一。雌性按蚊是人类疟疾的传播媒介。当蚊子叮咬受感染的个体时，它沿着血液携带寄生虫的传染形式。这种寄生虫通过其蚊子载体经历了一个复杂的旅程，然后可以通过蚊子叮咬将其注入下一个人类宿主。作为这一旅程的一部分，寄生虫必须穿过两个上皮细胞，蚊子的中肠和唾液腺。这项研究的目的是描述一种常见的蚊子上皮细胞对疟原虫入侵的免疫反应，其特征在于丝氨酸蛋白酶抑制剂serpin-6（SRPN 6）。 我们的目的是（a）探索SRPN 6在寄生虫入侵中肠和唾液腺期间的调节和（B）测量SRPN 6对寄生虫通过这些上皮的任何潜在作用。这项研究的结果可以提供重要的线索，在疾病相关的蚊子/寄生虫组合的蚊子媒介的寄生虫传播的上皮免疫的潜在贡献。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Despite ongoing control and prevention efforts, malaria remains one of the most devastating infectious diseases, world-wide. Female Anopheles mosquitoes are the vectors for human malaria. When a mosquito bites an infected individual, it takes up infectious forms of the parasite along with blood. The parasite undergoes a complex journey through its mosquito vector, before it can subsequently be injected into the next human host via mosquito bite. As part of this journey, the parasite must cross two epithelia, the mosquitoes' midgut and the salivary glands This study aims to characterize a common mosquito epithelial immune response against malaria parasite invasion, which is characterized by the serine protease inhibitor serpin-6 (SRPN6). Our aims are to (a) explore the regulation of SRPN6 during the invasion of the midgut and salivary glands by the parasite and (b) to measure any potential effect of SRPN6 on the passage of the parasite through these epithelia. The results of this study can provide important clues to the potential contribution of epithelial immunity to parasite transmission by mosquito vectors in a disease-relevant mosquito/parasite combination.