COBRE: RIH: CORE B: PROTEOMICS: PROTEIN BIOINFORMATICS, HU GENOME, DNA

COBRE：RIH：核心 B：蛋白质组学：蛋白质生物信息学、HU 基因组、DNA

• 批准号: 8167901
• 负责人: DJURO JOSIC
• 金额: $ 53.03万
• 依托单位: RHODE ISLAND HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167901
• 关键词: Bioinformatics; Biological Markers; Biological Process; Centers of Research Excellence; Chromatography; Complex; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Disease; Electrophoresis; Funding; Genome; Grant; Hospitals; Institution; Ligands; Liquid Chromatography; Longevity; Malignant Neoplasms; Maps; Mass Spectrum Analysis; Methods; Modification; NCI Center for Cancer Research; Peptides; Process; Protein Analysis; Proteins; Proteomics; Publications; Recording of previous events; Relative (related person); Research; Research Methodology; Research Personnel; Resources; Rhode Island; Role; Sampling; Services; Source; Translations; Two-Dimensional Gel Electrophoresis; United States National Institutes of Health; Universities; Western Blotting; instrumentation; method development; programs; research and development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Proteomics Core in the COBRE Center for Cancer Research Development (CCRD) at Rhode Island Hospital was established in September 2002 as part of the COBRE grant. The Core is currently offering LC-MS/MS and SELDI-TOF protein identification, biomarker identification with LC-MS/MS and SELDI-TOF mass spectrometry and 2-D electrophoresis, and chromatographic and 1- and 2-D electrophoretic separations and analysis of proteins and peptides as a service for four Rhode Island COBRE Centers, Rhode Island Hospital and other Lifespan Hospitals, Brown University and other neighboring institutions. Proteomics Core serves the following functions: 1) Identification of proteins isolated by 1- and 2-D gel electrophoresis, western blotting or chromatography. 2) Differential analysis and identification and relative quantification of up- or down-regulated proteins between two different samples with LC-MS/MS, SELDI-TOF MS and/or 2-D electrophoresis. 3) Study of protein-protein and protein-ligand interaction by use of liquid chromatography, SELDI-TOF MS and immunochemical methods combined with analyses with 1- and 2-D electrophoresis. 4) Isolation and identification of proteins associated in complexes to elucidate their biological function. 5) Identification and mapping of post-translation modifications and processing of target proteins. This renewal application describes: A) Specific Aim; B) History of the Proteomics Core facility, current instrumentation and operational plan for the facility; C) Core Progress including the role of the Proteomics Core had in fulfilling research objectives in COBRE-funded, NIH-funded and other research programs in Greater Providence and Rhode Island; Publications and other highlights; D) Experimental plan  to research the method development and identification of biomarkers for cancer and other diseases as well as plans and activities to obtain additional funding for the facility.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 罗德岛医院 COBRE 癌症研究发展中心 (CCRD) 的蛋白质组学核心于 2002 年 9 月成立，作为 COBRE 资助的一部分。 Core 目前正在为四个罗德岛 COBRE 中心、罗德岛医院和其他 Lifespan 医院、布朗大学和其他邻近机构提供 LC-MS/MS 和 SELDI-TOF 蛋白质鉴定、使用 LC-MS/MS 和 SELDI-TOF 质谱和 2-D 电泳进行的生物标志物鉴定，以及蛋白质和肽的色谱和 1- 和 2-D 电泳分离和分析。 蛋白质组学核心具有以下功能： 1) 通过一维和二维凝胶电泳、蛋白质印迹或层析鉴定分离的蛋白质。 2) 使用 LC-MS/MS、SELDI-TOF MS 和/或 2-D 电泳对两个不同样品之间上调或下调的蛋白质进行差异分析和鉴定以及相对定量。 3) 使用液相色谱、SELDI-TOF MS和免疫化学方法结合一维和二维电泳分析来研究蛋白质-蛋白质和蛋白质-配体相互作用。 4) 分离和鉴定复合物中相关的蛋白质，以阐明其生物学功能。 5) 目标蛋白翻译后修饰和加工的鉴定和定位。此续订申请描述： A) 具体目标； B) 蛋白质组学核心设施的历史、当前的仪器和设施的运营计划； C) 核心进展，包括蛋白质组学核心在实现 COBRE 资助、NIH 资助以及大普罗维登斯和罗德岛州其他研究项目的研究目标方面所发挥的作用；出版物和其他亮点； D) 研究癌症和其他疾病生物标志物的方法开发和鉴定的实验计划，以及为该设施获得额外资金的计划和活动。