Ultraspec III TOF/TOF 200 MALDI-TOF Mass Spectrometry Imaging System

描述（由申请人提供）：申请资金用于购买Ultraflex III TOF/TOF 200 MALDI-TOF质谱成像系统，这是一种最新的Ultraflex III TOF/TOF 200模块化飞行时间质谱仪以及相应的软件和附件，为组织分子成像提供样品制备。质谱成像系统也将用于蛋白质鉴定，但主要建议是参考（对照组织）和研究组织（主要来自主要用户组（MUG））之间不同蛋白质表达的分子成像和检测。该系统的分子成像和蛋白质鉴定将显着有利于研究和教学的努力，七个NIH资助的研究人员，其项目在此提交的详细信息。布朗大学校园的调查人员和布朗大学沃伦阿尔珀特医学院附属的五家寿命医院也将使用这种仪器。该仪器将位于蛋白质组学核心，这是NIH/NCRR资助的生物医学研究卓越中心（COBRE）癌症研究开发中心（CCRD）的一部分，也将由COBRE CCRD研究者使用。MUG的两名研究人员（希克森博士和莫斯博士）也是COBRE CCRD计划的成员。蛋白质组学核心实验室位于核心研究实验室（CRL）附近。Proteomics Core和CRL之间的紧密合作计划促进MALDI TOF/TOF系统在分子成像中的最佳使用。CRL实验室及其主任Paul McMillan博士在组织化学和分子成像样品制备方面的丰富经验也将成为这种最先进仪器的用户的非凡资源。目前有两个质谱仪在使用中的蛋白质组学核心。首先，QSTAR XL（Applied Biosystems/Sciex）用于电泳分离和凝胶内消化后的蛋白质鉴定，或用于更复杂样品（如细胞和细胞器，特别是质膜提取物）的凝胶内消化后的蛋白质鉴定。第二个是Ciphergen/Bio-Rad SELDI-TOF系统，用于生物标志物发现中的蛋白质谱分析。这些仪器成功地为80多名客户提供服务，这些客户主要来自Lifespan和布朗大学的科学界，也有来自美国其他学术界的其他客户。Ultraflex III TOF/TOF 200 MALDI-TOF质谱成像系统将补充和增强Proteomics Core质谱服务，从而为科学界提供以多种方式分析蛋白质的灵活性和广度，最终目标是发现疾病生物标志物和细胞分化生物标志物。我们的意图是，核心用户从这项技术中获得的研究成果将最终加速蛋白质组学相关的转化研究。所要求的仪器不仅将支持本申请中描述的七个主要用户组项目的转化和基础科学研究，还将支持五个生物化学研究卓越中心（COBRE）的研究人员，包括我们的中心（COBRE CCRD），罗得岛医院的COBRE Skeleton健康中心和布朗大学的COBRE中心，妇女和婴儿医院和罗杰威廉姆斯医疗中心，并将可能使布朗大学和6家附属教学医院的其他研究者受益。最后，Ultraflex III TOF/TOF 200 MALDI-TOF质谱成像系统将是罗得岛州唯一具有这种配置和这种应用的仪器，可向整个科学界开放。

DESCRIPTION (provided by applicant): Funds are requested for the acquisition of Ultraflex III TOF/TOF 200 MALDI- TOF Mass Spectrometry Imaging System, an up-to-date Ultraflex III TOF/TOF200 modular time-of-flight mass spectrometer together with corresponding software and accessories, to provide sample preparation for tissue molecular imaging. The mass spectrometry imaging system will also be used for protein identification, but the main propose will be the molecular imaging and detection in different protein expression between the reference (control tissue) and investigated tissue, mainly from the Major User Group (MUG). The system for molecular imaging and protein identification will significantly benefit the research and teaching endeavors of the seven NIH-funded investigators whose projects are detailed in this submission. Brown University campus- based investigators and of the five Lifespan Hospitals that are affiliated with the Brown University's Warren Alpert Medical School will also have access to this instrument. This instrument will be located in the Proteomics Core, which is part of the NIH/NCRR-funded Centers of Biomedical Research Excellence (COBRE) Center for Cancer Research Development (CCRD), and will be also used by the COBRE CCRD investigators. Two of the MUG investigators (Drs. Hixson and Moss) are also members of the COBRE CCRD program. Proteomics Core laboratory is located adjacent to Core Research Laboratories (CRL). A tight collaboration between the Proteomics Core and CRL is planned to foster optimal use of the MALDI TOF/TOF system for molecular imaging. The vast experience of the CRL laboratory and its Director, Dr. Paul McMillan, both in histochemistry and sample preparation for molecular imaging will also be an extraordinary resource to the users of this state-of-the-art instrument. Currently there are two mass spectrometers in use in the Proteomics Core. First, the QSTAR XL (Applied Biosystems/Sciex) is being used for protein identification after electrophoretic separation and in-gel digestion, or after in-gel digestion of more complex samples such as cell and organelles, especially plasma membrane extracts. The second one is the Ciphergen/Bio-Rad SELDI-TOF system, which is used for protein profiling in biomarker discovery. These instruments successfully serve a group of more than 80 costumers mostly from the Lifespan and Brown University scientific community, but also other customers from other academic communities throughout the United States. The Ultraflex III TOF/TOF 200 MALDI-TOF Mass Spectrometry Imaging System will complement and enhance the Proteomics Core mass spectrometry services, thus providing the scientific community with the flexibility and breadth to analyze proteins in numerous ways with the final goal to discover disease biomarkers and biomarkers for cellular differentiation. It is our intent that Core users' research findings from this technology will ultimately expedite proteomics-related translational research PUBLIC HEALTH RELEVANCE: The requested instrumentation will not only support the translational and basic science research of the seven Major User Group projects described in this application, but will also support investigators of five Center of Biochemical Research Excellence (COBRE), including our Center (COBRE CCRD), COBRE Center for Skeletal Health at the Rhode Island Hospital and COBRE Centers at the Brown University, Women and Infants Hospital and Roger Williams Medical Center, and will potentially benefit other investigators from Brown University and 6 affiliated teaching hospitals. Finally, the Ultraflex III TOF/TOF 200 MALDI-TOF Mass Spectrometry Imaging System will be the only instrument in this configuration and for this kind of application in the State of Rhode Island, with open access to the whole scientific community.