COBRE: RIH: CORE B: PROTEOMICS: PROTEIN BIOINFORMATICS, HU GENOME, DNA

这个子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 罗得岛医院的COBRE癌症研究发展中心（CCRD）的蛋白质组学核心于2002年9月成立，作为COBRE赠款的一部分。该核心目前正在提供LC-MS/MS和SELDI-TOF蛋白质鉴定，LC-MS/MS和SELDI-TOF质谱和2-D电泳的生物标志物鉴定，以及蛋白质和肽的色谱和1-和2-D电泳分离和分析，作为四个罗得岛COBRE中心，罗得岛医院和其他Lifespan医院，布朗大学和其他邻近机构的服务。 Proteomics Core具有以下功能：1）通过1-和2-D凝胶电泳，蛋白质印迹或色谱法鉴定分离的蛋白质。2)使用LC-MS/MS、SELDI-TOF MS和/或2-D电泳对两种不同样品之间上调或下调的蛋白质进行差异分析、鉴定和相对定量。3)通过液相色谱、SELDI-TOF MS和免疫化学方法结合一维和二维电泳分析研究蛋白质-蛋白质和蛋白质-配体相互作用。4)分离和鉴定复合物中相关的蛋白质，以阐明其生物学功能。5)翻译后修饰的鉴定和定位以及靶蛋白的加工。此续订申请描述： （一）具体目标; B）蛋白质组学核心设施的历史、设施的当前仪器和操作计划; C）核心进展，包括蛋白质组学核心在实现大普罗维登斯和罗得岛的COBRE-funded、NIH-funded和其他研究计划的研究目标中的作用;出版物和其他亮点; D）实验计划  研究癌症和其他疾病的生物标志物的方法开发和鉴定，以及为该设施获得额外资金的计划和活动。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Proteomics Core in the COBRE Center for Cancer Research Development (CCRD) at Rhode Island Hospital was established in September 2002 as part of the COBRE grant. The Core is currently offering LC-MS/MS and SELDI-TOF protein identification, biomarker identification with LC-MS/MS and SELDI-TOF mass spectrometry and 2-D electrophoresis, and chromatographic and 1- and 2-D electrophoretic separations and analysis of proteins and peptides as a service for four Rhode Island COBRE Centers, Rhode Island Hospital and other Lifespan Hospitals, Brown University and other neighboring institutions. Proteomics Core serves the following functions: 1) Identification of proteins isolated by 1- and 2-D gel electrophoresis, western blotting or chromatography. 2) Differential analysis and identification and relative quantification of up- or down-regulated proteins between two different samples with LC-MS/MS, SELDI-TOF MS and/or 2-D electrophoresis. 3) Study of protein-protein and protein-ligand interaction by use of liquid chromatography, SELDI-TOF MS and immunochemical methods combined with analyses with 1- and 2-D electrophoresis. 4) Isolation and identification of proteins associated in complexes to elucidate their biological function. 5) Identification and mapping of post-translation modifications and processing of target proteins. This renewal application describes: A) Specific Aim; B) History of the Proteomics Core facility, current instrumentation and operational plan for the facility; C) Core Progress including the role of the Proteomics Core had in fulfilling research objectives in COBRE-funded, NIH-funded and other research programs in Greater Providence and Rhode Island; Publications and other highlights; D) Experimental plan  to research the method development and identification of biomarkers for cancer and other diseases as well as plans and activities to obtain additional funding for the facility.