COBRE: RIH: CORE B: PROTEOMICS: PROTEIN BIOINFORMATICS, HU GENOME, DNA

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 位于罗德岛医院的科布雷癌症研究开发中心(CCRD)的蛋白质组学核心于2002年9月成立，作为科布雷赠款的一部分。该中心目前提供LC-MS/MS和SELDI-TOF蛋白质鉴定、LC-MS/MS和SELDI-TOF质谱仪生物标记物鉴定和二维电泳，以及蛋白质和多肽的层析、一维和二维电泳分离和分析，为四个罗德岛科布雷中心、罗德岛医院和其他寿命医院、布朗大学和其他邻近机构提供服务。 蛋白质组学核心具有以下功能：1)通过1-D和2-D凝胶电泳法、Western blotting或层析法分离的蛋白质进行鉴定。2)用LC-MS/MS、SELDI-TOF MS和/或双向电泳法对两个不同样品中上调或下调的蛋白质进行了差异分析、鉴定和相对定量。3)用高效液相色谱、SELDI-TOF MS和免疫化学方法研究蛋白质-蛋白质和蛋白质-配体的相互作用，并结合一、二维电泳法进行分析。4)分离和鉴定复合体中的相关蛋白，以阐明其生物学功能。5)翻译后修饰和靶蛋白加工的鉴定和定位。此续订申请描述： A)具体目标； B)蛋白质组学核心设施的历史、目前的仪器和设施的运作计划； C)核心进展，包括蛋白质组学核心在实现大普罗维登斯和罗德岛Cobre资助、NIH资助和其他研究方案的研究目标方面的作用；出版物和其他重点； D)研究癌症和其他疾病的生物标记物的方法、开发和鉴定的试验计划，以及为该设施获得额外资金的计划和活动。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Proteomics Core in the COBRE Center for Cancer Research Development (CCRD) at Rhode Island Hospital was established in September 2002 as part of the COBRE grant. The Core is currently offering LC-MS/MS and SELDI-TOF protein identification, biomarker identification with LC-MS/MS and SELDI-TOF mass spectrometry and 2-D electrophoresis, and chromatographic and 1- and 2-D electrophoretic separations and analysis of proteins and peptides as a service for four Rhode Island COBRE Centers, Rhode Island Hospital and other Lifespan Hospitals, Brown University and other neighboring institutions. Proteomics Core serves the following functions: 1) Identification of proteins isolated by 1- and 2-D gel electrophoresis, western blotting or chromatography. 2) Differential analysis and identification and relative quantification of up- or down-regulated proteins between two different samples with LC-MS/MS, SELDI-TOF MS and/or 2-D electrophoresis. 3) Study of protein-protein and protein-ligand interaction by use of liquid chromatography, SELDI-TOF MS and immunochemical methods combined with analyses with 1- and 2-D electrophoresis. 4) Isolation and identification of proteins associated in complexes to elucidate their biological function. 5) Identification and mapping of post-translation modifications and processing of target proteins. This renewal application describes: A) Specific Aim; B) History of the Proteomics Core facility, current instrumentation and operational plan for the facility; C) Core Progress including the role of the Proteomics Core had in fulfilling research objectives in COBRE-funded, NIH-funded and other research programs in Greater Providence and Rhode Island; Publications and other highlights; D) Experimental plan  to research the method development and identification of biomarkers for cancer and other diseases as well as plans and activities to obtain additional funding for the facility.