COBRE: RIH: CORE B: PROTEOMICS: PROTEIN BIOINFORMATICS, HU GENOME, DNA

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 蛋白质组学核心为COBRE癌症中心、位于罗得岛的其他COBRE中心和更大的学术界的成员的研究工作提供最先进的仪器和支持。我们的收费服务包括质谱分析，如LC/MS/MS和SELDI-TOF质谱，电泳分析，包括SDS-PAGE，等电聚焦，2-D电泳，免疫印迹和使用VersaDoc成像系统的图像分析，以及蛋白质和肽色谱和肽合成。我们还提供蛋白质纯化和表征以及小规模下游加工方面的专业知识。我们的设施位于RI医院的科罗中心。今年，共有23个研究小组使用了我们的服务和设施。目前有七位研究者使用我们实验室的仪器，主要是质谱仪、Ciphergen SELDI-TOF色谱系统来分离、鉴定和表征他们的样品。我们的两种质谱仪，ABI的QStar XL LC/MS/MS系统和Ciphergen的SELDI-TOF都是常规使用的。SELDI-TOF系统也可用于非蛋白质组学核心研究人员。我们还新培训了来自其他COBRE实验室的四名年轻研究人员使用该仪器。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Proteomics Core provides state-of-the art instrumentation and support for the research efforts of the members of this COBRE Cancer Center, other COBRE Centers located in Rhode Island and greater academic community. Our fee-based services include mass spectrometric analyses such as LC/MS/MS and SELDI-TOF mass spectrometry, electrophoretic analyses, including SDS-PAGE, isoelectric focusing, 2-D electrophoresis, immunoblot, and image analysis by use of the VersaDoc Imaging System, and protein and peptide chromatography and peptide synthesis. We also offer expertise in protein purification and characterization, and small-scale down stream processing. Our facility is located in the Coro Center at RI Hospital. In this year, a total of 23 research groups have made use of our services and facilities. Seven investigators currently use instruments in our laboratory, mainly the mass spectrometer, Ciphergen SELDI-TOF chromatographic systems for isolation, identification and characterization of their samples. Both of our mass spectrometers, a QStar XL LC/MS/MS system from ABI and SELDI-TOF from Ciphergen are in routine use. The SELDI-TOF system is also available for non-proteomics core investigators. We have also newly trained four young investigators from other COBRE laboratories to use this instrument.