ES Cell & Transgenic

这一共享资源为小鼠模型人类癌症的产生和研究的许多方面提供了收费服务支持。胚胎干细胞和转基因设施专门协助研究人员设计、生成、维护和分析含有设计突变的小鼠。工作人员还利用鼠标提供各种协议的熟练帮助 ES细胞(建立新的细胞系，分化为特定的细胞类型)。 科赫研究所的成员在使用老鼠模型研究分子方面一直处于领先地位。 肿瘤的发展基础和在癌症治疗中的耐药性日益增强。ES细胞和 转基因核心设施(以前的转基因动物核心)作为一个集中服务，以确保 所有Kl成员都可以使用鼠标建模技术。具体地说，核心提供 咨询服务，帮助KL调查人员设计他们的基因靶向和整体建模策略， 产生转基因的ES细胞(通过基因打靶或从胚泡细胞中分离) 并进行注射以产生嵌合和转基因小鼠。ES细胞与转基因核心 同时重视调查员教育，为KL研究员提供最先进的培训 在建立靶向ES细胞、转基因和突变小鼠模型所需的任何或所有程序中。 它还维护着一个有用的小鼠品系的储存库，包括表达Cre或FLP的品系 根据需要提供的组织特异性或诱导性启动子下的重组酶。超过了 在当前的资助期内，使用这一服务的Kl成员的数量急剧增加 核心。为确保及时提供服务，核心的ES Cell Suite 搬迁到一个更好的位置，我们增加了工作人员，T ES细胞和转基因核心 也继续扩展其服务，以保持领先地位。例如，它已经将 关于它接触到第一批通过四倍体胚胎产生ES细胞来源的小鼠的科学家 通过学习和优化这项技术来实现互补，从而可以作为一项健壮的服务提供。 提供一系列可靠、高效的服务，灵活地满足独特的需求， 作为一名敬业、有才华和热情的员工，ES细胞和转基因核心处于支持KL的有利位置 研究人员的目标是产生越来越精确的小鼠模型，并继续 利用尖端技术拓展到癌症研究的新领域。

This shared resource provides fee-for-service support for many aspects of the generation and study of mouse models human cancer. The ES Cell and Transgenics Facility specializes in assisting researchers with the design, generation, maintenance, and analysis of mice containing designer mutations. The staff also provides skilled assistance with a variety of protocols utilizing mouse ES cells (establishment of new lines, differentiation into specific cell types). Members of the Koch Institute have been leaders in the use of mouse models to study the molecular basis of the tumor development and increasingly in cancer treatment and resistance. The ES Cell & Transgenics Core Facility (formerly the Transgenic Animal Core) acts as a centralized service to ensure that all Kl members have access to mouse modeling technology. Specifically, the Core provides consultative services to help Kl investigators design their gene targeting and overall modeling strategy, produces genetically-modified ES cells (by either gene targeting or de novo isolation from blastocytes) and conducts injections to generate chimeric and transgenic mice. The ES Cell & Transgenics Core places equal emphasis on investigator education and provides state-of-the-art training to Kl researchers in any or all the procedures required to create targeted ES cells, transgenics, and mutant mouse models. It also maintains a repository of useful mouse strains, including strains expressing Cre or Flp recombinase under tissue-specific or inducible promoters, which are provided on demand. Over the current funding period, there has been a dramatic increase in the number of Kl members who use this Core. To ensure that services are provided in a timely manner, the Core's ES Cell Suite has been relocated to an improved location and we have increased the staff, the T ES Cell & Transgenics Core has also continued to expand its services to remain on the cutting edge. For example, it has capitalized on its access to the scientists who first generated ES cell-derived mice by tetraploid embryo complementation by learning and optimizing this technique so that can be offered as a robust service. With an excellent menu of reliable and efficient services, flexibility to accommodate unique requests and a dedicated, talented and enthusiastic staff, the ES Cell & Transgenics Core is well situated to support Kl investigators in their goal of generating increasingly precise mouse models and also to continue to expand into new areas of cancer research using cutting-edge techniques.