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ES Cell & Transgenic

胚胎干细胞

基本信息

批准号：
8181148
负责人：
RICHARD O HYNES
金额：
$ 16.02万
依托单位：
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-20 至 2015-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8181148
关键词：
A Mouse Animal Barrier Facility Animals Area Arts Backcrossings Breeding Cancer Center Cancer Center Support Grant Cataloging Catalogs Cell Culture Techniques Cell Differentiation process Cells Charge Chimera organism Collection Consultations Core Facility DNA Development Drug resistance ES Cell Line Education Embryo Ensure Fee-for-Service Plans Female Fibroblasts Fostering Freezing Funding Gene Silencing Gene Targeting Gene Transfer Techniques Generations Genes Genetic Genetic Recombination Genetic Screening Genomics Genotype Germ Lines Goals Harvest Histology Hybrids In Vitro Individual Injection of therapeutic agent Institutes Karyotype determination procedure Knock-out Laboratories Learning Location Maintenance Medicine Methodology Methods Microinjections Mission Missouri Modeling Molecular Mouse Strains Mus Mutation Oocytes Operative Surgical Procedures Pharmaceutical Preparations Phenotype Plasmids Procedures Production Protocols documentation Publications Reagent Recommendation Recording of previous events Reporter Research Personnel Resistance Resource Sharing Route Scientist Screening procedure Serum Services Site Southern Blotting Stem cells Subfamily lentivirinae Superovulation Techniques Technology Testing Time TimeLine Tissues Toxin Training Transgenic Animals Transgenic Mice Transgenic Organisms Universities Vertebral column Vial device Virus Weaning Work Workload animal facility anticancer research base blastocyst blastomere structure cancer therapy cell preparation cell type comparative design design and construction embryonic stem cell flexibility genetic strain human cancer mouse model implantation improved investigator training meetings member mouse model mutant mutant mouse model operation pathogen preimplantation promoter pup recombinase repository research study stem cell differentiation success transmission process tumor vector web site

项目摘要

This shared resource provides fee-for-service support for many aspects of the generation and study of mouse models human cancer. The ES Cell and Transgenics Facility specializes in assisting researchers with the design, generation, maintenance, and analysis of mice containing designer mutations. The staff also provides skilled assistance with a variety of protocols utilizing mouse ES cells (establishment of new lines, differentiation into specific cell types). Members of the Koch Institute have been leaders in the use of mouse models to study the molecular basis of the tumor development and increasingly in cancer treatment and resistance. The ES Cell & Transgenics Core Facility (formerly the Transgenic Animal Core) acts as a centralized service to ensure that all Kl members have access to mouse modeling technology. Specifically, the Core provides consultative services to help Kl investigators design their gene targeting and overall modeling strategy, produces genetically-modified ES cells (by either gene targeting or de novo isolation from blastocytes) and conducts injections to generate chimeric and transgenic mice. The ES Cell & Transgenics Core places equal emphasis on investigator education and provides state-of-the-art training to Kl researchers in any or all the procedures required to create targeted ES cells, transgenics, and mutant mouse models. It also maintains a repository of useful mouse strains, including strains expressing Cre or Flp recombinase under tissue-specific or inducible promoters, which are provided on demand. Over the current funding period, there has been a dramatic increase in the number of Kl members who use this Core. To ensure that services are provided in a timely manner, the Core's ES Cell Suite has been relocated to an improved location and we have increased the staff, the T ES Cell & Transgenics Core has also continued to expand its services to remain on the cutting edge. For example, it has capitalized on its access to the scientists who first generated ES cell-derived mice by tetraploid embryo complementation by learning and optimizing this technique so that can be offered as a robust service. With an excellent menu of reliable and efficient services, flexibility to accommodate unique requests and a dedicated, talented and enthusiastic staff, the ES Cell & Transgenics Core is well situated to support Kl investigators in their goal of generating increasingly precise mouse models and also to continue to expand into new areas of cancer research using cutting-edge techniques.

这种共享资源为小鼠模型人类癌症的生成和研究的许多方面提供了按服务收费的支持。ES细胞和转基因设施专门协助研究人员设计，生成，维护和分析含有设计者突变的小鼠。工作人员还提供熟练的援助与各种协议利用鼠标 ES细胞（建立新细胞系，分化为特定细胞类型）。科赫研究所的成员一直是使用小鼠模型研究分子生物学的领导者。肿瘤发展的基础，并越来越多地在癌症的治疗和耐药性。ES细胞& 转基因核心设施（以前的转基因动物核心）作为一个集中的服务，以确保所有的Kl成员都可以使用鼠标建模技术具体而言，核心提供咨询服务，以帮助KI研究人员设计他们的基因靶向和整体建模策略，产生基因修饰的ES细胞（通过基因靶向或从胚细胞中从头分离）并进行注射以产生嵌合和转基因小鼠。ES细胞和转基因核心同等重视研究者教育，并为Kl研究人员提供最先进的培训在任何或所有程序所需的建立有针对性的胚胎干细胞，转基因，和突变小鼠模型。它还保存了有用的小鼠品系，包括表达Cre或Flp的品系在一些实施方案中，重组酶在组织特异性或诱导型启动子下启动，其按需提供。来在当前的资助期间，使用此功能的Kl成员数量急剧增加核心为了确保及时提供服务，核心的ES Cell Suite已经搬迁到一个更好的位置，我们增加了工作人员，T ES细胞和转基因核心并继续扩展服务，以保持领先地位。例如，它获得了科学家谁第一次产生ES细胞衍生小鼠的四倍体胚胎通过学习和优化这种技术来补充，以便可以作为一种强大的服务提供。凭借可靠和高效的服务，灵活性，以满足独特的要求， ES细胞和转基因核心是一个专门的，有才华的和热情的工作人员，研究人员的目标是产生越来越精确的小鼠模型，并继续利用尖端技术扩展到癌症研究的新领域。