UHI COBRE: P1: MOL EPIDEMIOL & NATURAL HISTORY OF HPV IN HETEROSEXUAL MEN

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 人乳头瘤病毒（HPV）是性传播感染（STI）的主要原因，也是全球宫颈癌的主要原因。在亚太区域大部分地区，宫颈癌在所有妇女癌症中居首位。虽然现在有大量关于女性HPV感染的数据，但对异性恋男性HPV感染的自然史几乎一无所知。 缺乏这种知识阻碍了预防和控制妇女HPV感染的进展。该研究项目的目的是确定异性恋男性HPV感染的分子流行病学和自然史。我们的中心假设是，在女性中，HPV是一种常见的（高患病率和发病率），但在异性恋男性中通常是短暂的感染。我们进一步证实，年轻和低HPV病毒水平将与短暂感染相关，而年龄较大和高HPV病毒载量将预测病毒持续存在。拟议的研究将通过追求以下具体目标来检验我们的假设： 1.确定异性恋成年男性生殖器HPV感染的患病率和发病率。 2.确定异性恋成年男性生殖器HPV感染的相对短暂性和持续性。 3.确定与异性恋成年男性生殖器HPV感染的流行、偶发、短暂和持续相关的病毒和宿主因素。 一个由640名成年男性组成的队列将以4个月的间隔纵向随访24个月。将通过PCR分析生殖器（阴茎干、龟头/冠状沟、尿道）脱落细胞的HPV DNA。感染将通过HPV基因分型和变异分析来表征。此外，我们还将评估有关性史和性行为、避孕药具使用、性传播疾病史、吸烟和饮酒以及社会人口特征的信息。拟议的项目是创新的，因为它解决了目前知识中的一个重大空白。我们的期望是，我们将更好地了解男性HPV的自然史。这一新发现的知识将对改善未来HPV感染的预防策略产生重大影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Human papillomavirus (HPV) is a major cause of sexually transmitted infections (STI) and is the principal cause of cervical cancers worldwide. In much of the Asia-Pacific region, cervical carcinoma ranks first among all cancers in women. Although considerable data are now available about HPV infections in women, almost nothing is known about the natural history of HPV infections among heterosexual men. Lack of such knowledge has impeded progress in the prevention and control of HPV infection in women. The objective of this research project is to determine the molecular epidemiology and natural history of HPV infection in heterosexual men. Our central hypothesis is that, as in women, HPV is a common (high prevalence and incidence) but usually transient infection in heterosexual men. We further posit that young age and low HPV viral level will be associated with transient infections, while older age and high HPV viral load will predict viral persistence. The proposed study will test our hypotheses by pursuing the following specific aims: 1. Determine the prevalence and incidence of genital HPV infection in heterosexual adult men. 2. Determine the relative transience and persistence of genital HPV infection in heterosexual adult men. 3. Determine the viral and host factors associated with prevalent, incident, transient, and persistent genital HPV infection in heterosexual adult men. A cohort of 640 adult men will be followed longitudinally at four-month intervals for 24 months. Exfoliated cells from the genitalia (penile shaft, glans/coronal sulcus, urethra) will be analyzed for HPV DNA by PCR. Infections will be characterized by HPV genoyping and variant analysis. Also, we will evaluate information on sexual history and practices, contraceptive use, history of STI, tobacco and alcohol use, and sociodemographic characteristics. The proposed project is innovative, because it addresses a major gap in current knowledge. Our expectations are that we will have a better understanding about the natural history of HPV in men. This newfound knowledge will have a significant impact on improving future prevention strategies for HPV infection.