UHI COBRE: P1: MOL EPIDEMIOL & NATURAL HISTORY OF HPV IN HETEROSEXUAL MEN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 人乳头瘤病毒(HPV)是性传播感染(STI)的主要原因，也是全球宫颈癌的主要原因。在亚太地区的大部分地区，宫颈癌在所有女性癌症中排名第一。尽管现在有大量关于女性感染HPV的数据，但对异性恋男性感染HPV的自然历史几乎一无所知。 缺乏这方面的知识阻碍了在预防和控制妇女HPV感染方面的进展。本研究项目的目的是确定异性恋男性中HPV感染的分子流行病学和自然病史。我们的中心假设是，和女性一样，HPV在异性恋男性中是一种常见的(高发病率和高发病率)但通常是短暂的感染。我们进一步假设，年轻和低HPV病毒水平将与一过性感染有关，而年龄较大和高HPV病毒载量将预测病毒持久性。拟议的研究将通过追求以下具体目标来检验我们的假设： 1.确定异性成年男性生殖器HPV感染的患病率和发病率。 2.测定异性恋成年男性生殖器HPV感染的相对暂时性和持久性。 3.确定与异性恋成年男子生殖器HPV感染的流行、偶发、一过性和持久性有关的病毒和宿主因素。 一组640名成年男性将每隔4个月进行一次纵向追踪，为期24个月。生殖器(阴茎干、龟头/冠沟、尿路)的脱落细胞将用聚合酶链式反应分析HPVDNA。感染将通过HPV基因分型和变异分析来表征。此外，我们还将评估有关性史和性行为、避孕用法、性传播感染史、烟酒使用和社会人口学特征的信息。拟议的项目是创新的，因为它解决了当前知识的一个主要缺口。我们的期望是，我们将对男性HPV的自然历史有更好的了解。这一新发现的知识将对改进未来的HPV感染预防策略产生重大影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Human papillomavirus (HPV) is a major cause of sexually transmitted infections (STI) and is the principal cause of cervical cancers worldwide. In much of the Asia-Pacific region, cervical carcinoma ranks first among all cancers in women. Although considerable data are now available about HPV infections in women, almost nothing is known about the natural history of HPV infections among heterosexual men. Lack of such knowledge has impeded progress in the prevention and control of HPV infection in women. The objective of this research project is to determine the molecular epidemiology and natural history of HPV infection in heterosexual men. Our central hypothesis is that, as in women, HPV is a common (high prevalence and incidence) but usually transient infection in heterosexual men. We further posit that young age and low HPV viral level will be associated with transient infections, while older age and high HPV viral load will predict viral persistence. The proposed study will test our hypotheses by pursuing the following specific aims: 1. Determine the prevalence and incidence of genital HPV infection in heterosexual adult men. 2. Determine the relative transience and persistence of genital HPV infection in heterosexual adult men. 3. Determine the viral and host factors associated with prevalent, incident, transient, and persistent genital HPV infection in heterosexual adult men. A cohort of 640 adult men will be followed longitudinally at four-month intervals for 24 months. Exfoliated cells from the genitalia (penile shaft, glans/coronal sulcus, urethra) will be analyzed for HPV DNA by PCR. Infections will be characterized by HPV genoyping and variant analysis. Also, we will evaluate information on sexual history and practices, contraceptive use, history of STI, tobacco and alcohol use, and sociodemographic characteristics. The proposed project is innovative, because it addresses a major gap in current knowledge. Our expectations are that we will have a better understanding about the natural history of HPV in men. This newfound knowledge will have a significant impact on improving future prevention strategies for HPV infection.