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Transgenic Mouse

转基因小鼠

基本信息

批准号：
8180600
负责人：
MARK A MAGNUSON
金额：
$ 9.44万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-10 至 2015-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8180600
关键词：
Age Alleles Animals Award Cancer Center Cancer Center Support Grant Cells Collaborations Complex Consultations Cryopreservation DNA Disease Electroporation Embryo Embryo Transfer Employee Ensure Evaluation Fee-for-Service Plans Female Fertilization Fertilization in Vitro Freezing Funding Future Ganciclovir Gene Targeting Generations Genes Genetic Guidelines Harvest Human Human Resources Implant Individual Knock-in Mouse Laboratories Leadership Liquid substance Maintenance Malignant Neoplasms Methods Microinjections Modification Monitor Mus Nitrogen Outcome Ovarian Ovary Partner in relationship Plasmids Pluripotent Stem Cells Policies Preparation Price Principal Investigator Procedures Protocols documentation Puromycin Quality Control Research Personnel Resource Sharing Resources Risk Schedule Screening procedure Services Site-Directed Mutagenesis Solid Southern Blotting Sterility Techniques Technology Testing Time Training Transgenes Transgenic Mice Transgenic Organisms Translating Transplantation Vial device animal facility antibiotic G 418 assisted reproduction base blastocyst data management design embryo cell embryonic stem cell fusion gene genome-wide human cancer mouse model hygromycin A improved male member mouse genome mouse model multidisciplinary ovary transplantation pathogen prevent pup reconstitution research study sperm cell vector web site zygote

项目摘要

The Transgenic Mouse/ES Cell Shared Resource has been in existence since 1993 and has received continuous funding from the NCI since the Vanderbilt-lngram Cancer Center (VICC) was awarded its first Cancer Center Support Grant in 1995. At the time of the last competitive renewal, this shared resource received an "Outstanding" rating. This resource provides gene targeting, DNA pronuclear microinjections, ES cell microinjections, assisted reproduction and both sperm and embryo cryopreservation services to members of the VICC. These services are essential for the generation, maintenance, and long-term storage of germline-altered mice. The resource continues to function in a multidisciplinary manner and has undergone several significant changes since the last competitive evaluation that have enabled it to continue to provide gene targeting and microinjection services at reasonable prices, to develop several new services, and to improve both its quality control and data management strategies. The Transgenic Mouse/ES Cell Shared Resource continues to offer services that remain in high demand and vital to the generation of new mouse models of cancer and other diseases. This will be critical as the gene-specific information from genome-wide cancer studies is translated to mouse models of human cancer. Moreover, the technology base that this resource supports is expected to be important in allowing Vanderbilt investigators to adapt to using of human pluripotent stem cells in the future.

转基因小鼠/胚胎干细胞共享资源自 1993 年以来一直存在，自范德比尔特-英格拉姆癌症中心 (VICC) 于 1995 年获得第一笔癌症中心支持拨款以来，该资源一直获得 NCI 的持续资助。在上次竞争性更新时，该共享资源获得了“杰出”评级。该资源提供基因靶向、DNA 原核显微注射、ES 细胞显微注射、辅助生殖以及精子和胚胎冷冻保存服务 VICC 成员。这些服务对于种系改变小鼠的产生、维持和长期储存至关重要。该资源继续以多学科的方式运作，自上次竞争性评估以来经历了一些重大变化，使其能够继续以合理的价格提供基因靶向和显微注射服务，开发多种新服务，并改进其质量控制和数据管理策略。转基因小鼠/胚胎干细胞共享资源继续提供需求量很大且对新一代细胞至关重要的服务。癌症和其他疾病的小鼠模型。这将是至关重要的，因为来自全基因组癌症研究的基因特异性信息被转化为人类癌症的小鼠模型。此外，该资源支持的技术基础预计对于范德比尔特研究人员未来适应人类多能干细胞的使用非常重要。