Immunology Flow Cytometry Core

免疫学流式细胞术核心

• 批准号: 7925273
• 负责人: Denise L Faustman
• 金额: $ 24.32万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7925273
• 关键词: Antibodies; Area; Arts; Automation; Award; Biological Assay; Blood; Boston; Cell Separation; Cells; Collaborations; Communities; Complex; Computer Assisted; Consult; DNA purification; Data; Development; Diabetes Mellitus; Education; Endocrinology; Engineering; Flow Cytometry; General Hospitals; Goals; Hand; Home environment; Human; Immunologic Techniques; Immunologics; Immunology; Institution; Insulin-Dependent Diabetes Mellitus; Label; Laboratory Research; Lymphocyte; Manuals; Massachusetts; Methods; Mus; Non-Insulin-Dependent Diabetes Mellitus; Phase; Preparation; Process; Research; Research Design; Research Personnel; Robotics; Sampling; Science; Scientist; Services; Sorting - Cell Movement; Standardization; Surveys; T-Lymphocyte; Techniques; Technology Transfer; Training; Training and Education; Translations; Universities; Work; base; color detection; cost effective; cost effectiveness; cost efficient; design; double-blind placebo controlled trial; improved; magnetic cell separation; magnetic field; medical schools; medical specialties; operation; research study; type I and type II diabetes

The Boston-Cambridge area is home to a large research community of diverse scientists studying Type I and Type II diabetes. At present, many investigators are attempting to carry out techniques in their own laboratories for research goals that in many cases are no longer their primary area of specialty. This is particularly true for flow cytometry and complex separations of lymphocytes with the need for tight controls on the quality of the preparations. Importantly, few flow cytometry cores are dedicated to investigators involved in diabetes research. The purpose of this Immunology Core of the Boston Area Diabetes Endocrinology Research Center (BADERC) is to financially improve and scientifically expedite the translation of excellent ongoing diabetes research through a cost efficient mechanism. This has been successfully accomplished by the Immunology Core, which has been highly utilized over the past 5 years. The Immunology Core has provided strong education to investigators and a diversity of state-of-the-art flow cytometric techniques and new robotic methods for lymphocyte separations. Remarkably, at the entire Massachusetts General Hospital, Harvard Medical School as well as Boston University no flow cytometry core for diabetes users and collaborators and for their applications central to Type 1 and Type II diabetes research existed prior to the BADERC. The Immunology Flow Cytometry Core has provided these fundamental services to the Boston-Cambridge diabetes based research community including: .Flow cytometer access, data preparation and interpretations .Flow cytometry training, education and experiment design assistance. .Flow cytometry sample analysis with sorting, multi-color detection, fluorchrome labeling, antibody purifications, DNA and Ca flux experiments, etc. .Manual and robotic human blood lymphocyte separations for standardizations of assays using robotics. These Center services have been very cost effective as well as to provide rigorous training in Immunology.

波士顿-剑桥地区是一个由研究I型和II型的不同科学家组成的大型研究社区的所在地。 II型糖尿病。目前，许多研究人员正试图在他们自己的实验室中进行技术研究， 研究目标，在许多情况下，不再是他们的主要专业领域。对于心流来说尤其如此 细胞计数和淋巴细胞的复杂分离，需要严格控制细胞的质量， 准备工作。重要的是，很少有流式细胞术核心是专门用于参与糖尿病研究的研究人员。 波士顿地区糖尿病内分泌研究中心（BADERC）的免疫学核心的目的是通过具有成本效益的机制，在经济上改善和科学上加快正在进行的优秀糖尿病研究的转化。这已经成功地完成了免疫学核心，这在过去5年中得到了高度利用。免疫学核心为研究人员提供了强有力的教育，并提供了多种最先进的流式细胞术技术和淋巴细胞分离的新机器人方法。值得注意的是，在整个马萨诸塞州总医院、哈佛医学院以及波士顿大学，在BADERC之前，没有针对糖尿病用户和合作者的流式细胞术核心，也没有针对1型和II型糖尿病研究的核心应用。免疫学流式细胞术核心提供了这些 为波士顿-剑桥糖尿病研究社区提供基本服务，包括： .流式细胞仪访问、数据准备和解释 ·流式细胞仪培训、教育和实验设计协助。 ·流式细胞仪样品分析，包括分选、多色检测、荧光染料标记、抗体纯化、DNA和Ca通量实验等。 ·手动和机器人血液淋巴细胞分离，用于使用机器人技术进行分析。 这些中心的服务非常具有成本效益，并提供严格的免疫学培训。