THE GLYCOME OF SIALYLATION MUTANTS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 为支持NCRR生物医学糖组学赠款的目标而开发的技术适用于许多不同的系统。 用于聚糖表征的非偏倚方法具有足够的灵敏度，可检测少量组织中的次要聚糖。 作为对这些方法的挑战，我们已着手检测黑腹果蝇野生型和突变株中的唾液酸化聚糖加工中间体，黑腹果蝇是一种产生极少量唾液酸化聚糖的生物体。 Panin实验室已经积累了CMP-唾液酸的生产、运输和利用所必需的酶的突变体，以及制造果蝇唾液酸转移酶受体聚糖结构的酶的突变体。 Tiemeyer实验室分析了Panin实验室提供的突变组织的聚糖谱。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Technology developed in support of the aims of the NCRR Biomedical Glycomics grant is applicable to many different systems. The non-biased methods for glycan characterization are sufficiently sensitive to allow detection of minor glycans from small amount of tissue. As a challenge to these methods, we have undertaken the detection of sialylated glycan processing intermediates in wild-type and mutant strains of Drosophila melanogaster, an organism that produces very small amounts of sialylated glycans. The Panin lab has amassed mutants in enzymes necessary for the production, transport, and utilization of CMP-sialic acid, as well as in enzymes that make glycan structures that are acceptors for the Drosophila sialyltransferase. The Tiemeyer lab has analyzed glycan profiles for mutant tissues provided by the Panin lab.