THE GLYCOME OF SIALYLATION MUTANTS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 为支持NCRR生物医学血糖基金的目标而开发的技术适用于许多不同的系统。非偏倚的多聚糖鉴定方法具有足够的灵敏度，可以从少量组织中检测到少量的多聚糖。作为对这些方法的挑战，我们已经在野生型和突变品系的黑腹果蝇中检测到唾液酸化多糖加工中间体，这是一种产生非常少量唾液酸化多糖的有机体。Panin实验室已经积累了生产、运输和利用CMP-唾液酸所必需的酶的突变株，以及产生果蝇唾液酸转移酶受体的糖链结构的酶。蒂迈耶实验室已经分析了Panin实验室提供的突变组织的糖链图谱。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Technology developed in support of the aims of the NCRR Biomedical Glycomics grant is applicable to many different systems. The non-biased methods for glycan characterization are sufficiently sensitive to allow detection of minor glycans from small amount of tissue. As a challenge to these methods, we have undertaken the detection of sialylated glycan processing intermediates in wild-type and mutant strains of Drosophila melanogaster, an organism that produces very small amounts of sialylated glycans. The Panin lab has amassed mutants in enzymes necessary for the production, transport, and utilization of CMP-sialic acid, as well as in enzymes that make glycan structures that are acceptors for the Drosophila sialyltransferase. The Tiemeyer lab has analyzed glycan profiles for mutant tissues provided by the Panin lab.