ANALYSIS OF GLYCOPROTEIN & GLYCOLIPID GLYCAN EXPRESSION OF STEM CELLS

这个子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心拨款提供。次级项目的主要支助 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 利用资源中心开发的几种技术，我们详细研究了人类和小鼠胚胎干细胞谱系中糖蛋白和糖脂聚糖的相对表达模式。 重点是识别细胞分化为其他细胞类型时的显著变化。 此外，我们还比较了几种不同干细胞系以及iPS细胞中的聚糖。 我们还在开发工具，以增强高通量糖组学分析和MS数据的自动注释。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Using several technologies developed in the Resource Center, we have investigated in detail the relative expression patterns of glycoprotein and glycolipid glycans in both human and mouse embryonic stem cell lineages. The emphasis is on identifying significant changes when cells are differentiated into other cell types. In addition, we have compared the glycans in several different stem cell lines, as well as in iPS cells. We are also developing tools to enhance high-throughput glycomic analysis and automated annotation of MS data.