ANALYSIS OF GLYCOPROTEIN & GLYCOLIPID GLYCAN EXPRESSION OF STEM CELLS

糖蛋白分析

• 批准号: 8363050
• 负责人: MICHAEL TIEMEYER
• 金额: $ 5.16万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363050
• 关键词: Automated Annotation; Cell Line; Cell Lineage; Cells; Data; Funding; Glycolipids; Glycoproteins; Grant; Human; Mus; National Center for Research Resources; Pattern; Polysaccharides; Principal Investigator; Relative (related person); Research; Research Infrastructure; Resources; Source; Stem cells; Technology; United States National Institutes of Health; cell type; cost; embryonic stem cell; induced pluripotent stem cell; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Using several technologies developed in the Resource Center, we have investigated in detail the relative expression patterns of glycoprotein and glycolipid glycans in both human and mouse embryonic stem cell lineages. The emphasis is on identifying significant changes when cells are differentiated into other cell types. In addition, we have compared the glycans in several different stem cell lines, as well as in iPS cells. We are also developing tools to enhance high-throughput glycomic analysis and automated annotation of MS data.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 利用资源中心开发的几种技术，我们详细研究了人类和小鼠胚胎干细胞谱系中糖蛋白和糖脂聚糖的相对表达模式。 重点是识别细胞分化为其他细胞类型时的显著变化。 此外，我们还比较了几种不同干细胞系以及iPS细胞中的聚糖。 我们还在开发工具，以增强高通量糖组学分析和MS数据的自动注释。