ANALYSIS OF GLYCOPROTEIN & GLYCOLIPID GLYCAN EXPRESSION OF STEM CELLS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 利用资源中心开发的几项技术，我们详细研究了糖蛋白和糖脂多糖在人和小鼠胚胎干细胞谱系中的相对表达模式。重点是当细胞分化成其他类型的细胞时，识别显著的变化。此外，我们还比较了几种不同干细胞系以及iPS细胞中的葡聚糖。我们还在开发工具，以增强高通量血糖分析和MS数据的自动注释。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Using several technologies developed in the Resource Center, we have investigated in detail the relative expression patterns of glycoprotein and glycolipid glycans in both human and mouse embryonic stem cell lineages. The emphasis is on identifying significant changes when cells are differentiated into other cell types. In addition, we have compared the glycans in several different stem cell lines, as well as in iPS cells. We are also developing tools to enhance high-throughput glycomic analysis and automated annotation of MS data.