THE GLYCOME OF SIALYLATION MUTANTS

唾液酸化突变体的糖蛋白

• 批准号: 8363037
• 负责人: MICHAEL TIEMEYER
• 金额: $ 0.34万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363037
• 关键词: Cytidine Monophosphate N-Acetylneuraminic Acid; Detection; Drosophila genus; Drosophila melanogaster; Enzymes; Funding; Grant; Methods; Minor; National Center for Research Resources; Organism; Polysaccharides; Principal Investigator; Process; Production; Research; Research Infrastructure; Resources; Sialyltransferases; Source; Structure; System; Technology; Tissues; United States National Institutes of Health; cost; mutant; sialylation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Technology developed in support of the aims of the NCRR Biomedical Glycomics grant is applicable to many different systems. The non-biased methods for glycan characterization are sufficiently sensitive to allow detection of minor glycans from small amount of tissue. As a challenge to these methods, we have undertaken the detection of sialylated glycan processing intermediates in wild-type and mutant strains of Drosophila melanogaster, an organism that produces very small amounts of sialylated glycans. The Panin lab has amassed mutants in enzymes necessary for the production, transport, and utilization of CMP-sialic acid, as well as in enzymes that make glycan structures that are acceptors for the Drosophila sialyltransferase. The Tiemeyer lab has analyzed glycan profiles for mutant tissues provided by the Panin lab.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 为支持NCRR生物医学糖组学赠款的目标而开发的技术适用于许多不同的系统。 用于聚糖表征的非偏倚方法具有足够的灵敏度，可检测少量组织中的次要聚糖。 作为对这些方法的挑战，我们已着手检测黑腹果蝇野生型和突变株中的唾液酸化聚糖加工中间体，黑腹果蝇是一种产生极少量唾液酸化聚糖的生物体。 Panin实验室已经积累了CMP-唾液酸的生产、运输和利用所必需的酶的突变体，以及制造果蝇唾液酸转移酶受体聚糖结构的酶的突变体。 Tiemeyer实验室分析了Panin实验室提供的突变组织的聚糖谱。