TOXIN INTERACTIONS WITH TRPV1

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 分析离子通道结构/功能的最有力工具之一是鉴定天然毒素、激动剂和拮抗剂。几种生物会产生作用于离子通道的小蛋白毒素，帮助猎物捕获或防御。我们建议研究毒素与离子通道TRPV1的相互作用。我们将使用MALDI质谱仪来评估通过反相色谱分离的毒素的分子组成。加州大学旧金山分校的质谱学设施将协助收集以确定分离出的毒素的分子量。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. One of the most powerful tools for analyzing ion channel structure/function has been the identification of natural toxins agonists and antagonists. Several organisms produce small protein toxins that act on ion channels and aid in prey capture or defense. We propose to study toxin interaction with the ion channel TRPV1. We will use MALDI mass spectrometry to assess the molecular composition of toxins isolated by reverse phase chromatography. The UCSF Mass Spectrometry Facility will assist in the collection to determine the molecular weight of isolated toxins.