TOXIN INTERACTIONS WITH TRPV1

毒素与 TRPV1 的相互作用

• 批准号: 8169776
• 负责人: David Julius
• 金额: $ 3.18万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-12 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169776
• 关键词: Agonist; Collection; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; Ion Channel; Mass Spectrum Analysis; Molecular; Molecular Weight; Organism; Proteins; Research; Research Personnel; Resources; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Structure; TRPV1 gene; Toxin; United States National Institutes of Health; reversed phase chromatography; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. One of the most powerful tools for analyzing ion channel structure/function has been the identification of natural toxins agonists and antagonists. Several organisms produce small protein toxins that act on ion channels and aid in prey capture or defense. We propose to study toxin interaction with the ion channel TRPV1. We will use MALDI mass spectrometry to assess the molecular composition of toxins isolated by reverse phase chromatography. The UCSF Mass Spectrometry Facility will assist in the collection to determine the molecular weight of isolated toxins.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 用于分析离子通道结构/功能的最有力的工具之一是鉴定天然毒素激动剂和拮抗剂。 几种生物产生作用于离子通道的小蛋白质毒素，帮助捕获或防御猎物。 我们建议研究毒素与离子通道TRPV 1的相互作用。 我们将使用MALDI质谱法来评估通过反相色谱分离的毒素的分子组成。 UCSF质谱设施将协助收集，以确定分离毒素的分子量。