TRP CHANNEL MODULATION

TRP 通道调制

• 批准号: 8363787
• 负责人: David Julius
• 金额: $ 0.45万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363787
• 关键词: Binding; Biochemical; Chemicals; Cytoplasmic Protein; Cytoplasmic Tail; Esthesia; Funding; Future; Grant; Mass Spectrum Analysis; Membrane Proteins; National Center for Research Resources; Pain; Peptides; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Site; Source; United States National Institutes of Health; cost; crosslink; small molecule

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The purpose of this project is to perform biochemical characterization of proteins that modulate the activity of channels important for pain sensation. The targets of study will include channel cytoplasmic domains, small molecule modulators, peptide modulators, and associated membrane or cytoplasmic proteins. The objective is to purify proteins, determine molecular interaction sites, and perform structural studies.The UCSF mass spectrometry facility will aid us in determining the accurate mass and of purified products, as well as confirming the identity of purified products. The aid of the facility will also allow us to interpret results from chemical crosslinking studies in the future.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 本项目的目的是对调节痛觉重要通道活性的蛋白质进行生物化学表征。 研究的目标将包括通道胞质结构域、小分子调节剂、肽调节剂和相关的膜或胞质蛋白。 目的是纯化蛋白质，确定分子相互作用位点，并进行结构研究。UCSF质谱设备将帮助我们确定纯化产物的准确质量和纯度，以及确认纯化产物的身份。 该设施的帮助也将使我们能够解释未来化学交联研究的结果。