TOXIN INTERACTIONS WITH TRPV1

毒素与 TRPV1 的相互作用

• 批准号: 8363781
• 负责人: David Julius
• 金额: $ 0.03万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363781
• 关键词: Agonist; Collection; Funding; Grant; Ion Channel; Mass Spectrum Analysis; Molecular; Molecular Weight; National Center for Research Resources; Organism; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Structure; TRPV1 gene; Toxin; United States National Institutes of Health; cost; reversed phase chromatography; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. One of the most powerful tools for analyzing ion channel structure/function has been the identification of natural toxins agonists and antagonists. Several organisms produce small protein toxins that act on ion channels and aid in prey capture or defense. We propose to study toxin interaction with the ion channel TRPV1. We will use MALDI mass spectrometry to assess the molecular composition of toxins isolated by reverse phase chromatography. The UCSF Mass Spectrometry Facility will assist in the collection to determine the molecular weight of isolated toxins.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 分析离子通道结构/功能的最有力工具之一是鉴定天然毒素、激动剂和拮抗剂。几种生物会产生作用于离子通道的小蛋白毒素，帮助猎物捕获或防御。我们建议研究毒素与离子通道TRPV1的相互作用。我们将使用MALDI质谱仪来评估通过反相色谱分离的毒素的分子组成。加州大学旧金山分校的质谱学设施将协助收集以确定分离出的毒素的分子量。