USING MASS SPEC TO DETERMINE MOLECULAR IDENTITY OF TUMOR SPECIFIC ANTIGEN

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 抗体噬菌体展示是开发针对已知和新的肿瘤抗原的全人单克隆抗体的有用方法。我们已经获得了超过70种噬菌体抗体，其特异性结合前列腺癌细胞并在前列腺癌细胞内内化。揭示它们的分子身份将有助于更好地理解癌症生物学，并更好地评估这些分子作为靶向免疫治疗的靶点。我们建议使用免疫沉淀和串联质谱来获得抗体所靶向的抗原的肽序列。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Antibody phage display is a useful method of developing fully human monoclonal antibodies against both known and novel tumor antigens. We have obtained over 70 phage antibodies that specifically bind to and internalize within prostate cancer cells. Uncovering their molecular identities will lead to better understanding of cancer biology and better assessment of these molecules as targets for targeted immunotherapeutics. We propose to use immunoprecipitation and tandem mass spectrometry to obtain peptide sequences of antigens targeted by the antibodies.