USING MASS SPEC TO DETERMINE MOLECULAR IDENTITY OF TUMOR SPECIFIC ANTIGEN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 抗体噬菌体展示是制备针对已知和新的肿瘤抗原的全人单抗的有效方法。我们已经获得了70多个能与前列腺癌细胞结合并内化的噬菌体抗体。揭示它们的分子身份将有助于更好地理解癌症生物学，并更好地评估这些分子作为靶向免疫疗法的靶点。我们建议使用免疫沉淀和串联质谱学来获得抗体靶向的抗原的多肽序列。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Antibody phage display is a useful method of developing fully human monoclonal antibodies against both known and novel tumor antigens. We have obtained over 70 phage antibodies that specifically bind to and internalize within prostate cancer cells. Uncovering their molecular identities will lead to better understanding of cancer biology and better assessment of these molecules as targets for targeted immunotherapeutics. We propose to use immunoprecipitation and tandem mass spectrometry to obtain peptide sequences of antigens targeted by the antibodies.