USING MASS SPEC TO DETERMINE MOLECULAR IDENTITY OF TUMOR SPECIFIC ANTIGEN
USING MASS SPEC TO DETERMINE MOLECULAR IDENTITY OF TUMOR SPECIFIC ANTIGEN
批准号:
8169743
负责人:
James D. Marks
金额:
$0.18万
依托单位国家:
美国
项目类别:
财政年份:
2010
资助国家:
美国
项目状态:
已结题
起止时间:
2010-09-12 至 2011-05-31
关键词:
AntibodiesAntigen TargetingBacteriophagesBindingCancer BiologyComputer Retrieval of Information on Scientific Projects DatabaseFundingGrantImmunoprecipitationImmunotherapeutic agentInstitutionLeadMalignant neoplasm of prostateMethodsMolecularPhage DisplayResearchResearch PersonnelResourcesSourceTumor AntigensUnited States National Institutes of Healthcancer cellhuman monoclonal antibodiesnovelprotein aminoacid sequencetandem mass spectrometry
中文摘要
该子项目是利用
由NIH/NCRR资助的中心赠款提供的资源。子项目和
研究者(PI)可能从另一个NIH来源获得主要资金,
因此可以在其他CRISP条目中表示。列出的机构是
中心,不一定是研究者的机构。
抗体噬菌体展示是开发针对已知和新的肿瘤抗原的全人单克隆抗体的有用方法。我们已经获得了超过70种噬菌体抗体,其特异性结合前列腺癌细胞并在前列腺癌细胞内内化。揭示它们的分子身份将有助于更好地理解癌症生物学,并更好地评估这些分子作为靶向免疫治疗的靶点。我们建议使用免疫沉淀和串联质谱来获得抗体所靶向的抗原的肽序列。
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Antibody phage display is a useful method of developing fully human monoclonal antibodies against both known and novel tumor antigens. We have obtained over 70 phage antibodies that specifically bind to and internalize within prostate cancer cells. Uncovering their molecular identities will lead to better understanding of cancer biology and better assessment of these molecules as targets for targeted immunotherapeutics. We propose to use immunoprecipitation and tandem mass spectrometry to obtain peptide sequences of antigens targeted by the antibodies.
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会议论文
Renewable antibodies to secreted proteins and single and multi-pass cell surface
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批准号:8702409
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项目类别:
-
资助金额:$40.94万
-
财政年份:2014
-
负责人:James D. Marks
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依托单位:
Antibody Research Technology Center
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批准号:8666864
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项目类别:
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资助金额:$154.95万
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财政年份:2014
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负责人:James D. Marks
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依托单位:
Generation of therapeutic antibodies to serotype F botulism
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批准号:8608996
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项目类别:
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资助金额:$114.01万
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财政年份:2013
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负责人:James D. Marks
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依托单位:
Generation of therapeutic antibodies to serotype F botulism
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批准号:8996674
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项目类别:
-
资助金额:$101.3万
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财政年份:2013
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负责人:James D. Marks
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依托单位:
Generation of therapeutic antibodies to serotype F botulism
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批准号:8790945
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项目类别:
-
资助金额:$114.38万
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财政年份:2013
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负责人:James D. Marks
-
依托单位:
Generation of therapeutic antibodies to serotype F botulism
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批准号:8474647
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项目类别:
-
资助金额:$120.0万
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财政年份:2013
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负责人:James D. Marks
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依托单位:
Trispecific monoclonal antibody for botulinum neurotoxin intoxication therapy
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批准号:8367214
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项目类别:
-
资助金额:$20.78万
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财政年份:2012
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负责人:James D. Marks
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依托单位:
Trispecific monoclonal antibody for botulinum neurotoxin intoxication therapy
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批准号:8469825
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项目类别:
-
资助金额:$20.1万
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财政年份:2012
-
负责人:James D. Marks
-
依托单位:
Trispecific monoclonal antibody for botulinum neurotoxin intoxication therapy
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批准号:8875583
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项目类别:
-
资助金额:$45.15万
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财政年份:2012
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负责人:James D. Marks
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依托单位:
Trispecific monoclonal antibody for botulinum neurotoxin intoxication therapy
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批准号:8839870
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项目类别:
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资助金额:$43.83万
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财政年份:2012
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负责人:James D. Marks
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依托单位:
Development of botulinum neurotoxin immunotherapy, serotypes C, D, F, and G
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批准号:8547992
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项目类别:
-
资助金额:$8.7万
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财政年份:2012
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负责人:James D. Marks
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依托单位:
USING MASS SPEC TO DETERMINE MOLECULAR IDENTITY OF TUMOR SPECIFIC ANTIGEN
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批准号:8363749
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项目类别:
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资助金额:$0.0万
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财政年份:2011
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负责人:James D. Marks
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依托单位:
Evolving Diagnostic Antibodies for Botulinum Neurotoxins
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批准号:8260256
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项目类别:
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资助金额:$30.77万
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财政年份:2011
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负责人:James D. Marks
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依托单位:
Design and Generation of Cross- Reactive Botulinum Neurotoxin Antibodies
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批准号:7675182
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项目类别:
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资助金额:$29.71万
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财政年份:2009
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负责人:James D. Marks
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依托单位:
USING MASS SPEC TO DETERMINE MOLECULAR IDENTITY OF TUMOR SPECIFIC ANTIGEN
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批准号:7957383
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项目类别:
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资助金额:$0.0万
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财政年份:2009
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负责人:James D. Marks
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依托单位:
Development of botulinum neurotoxin immunotherapy, serotypes C, D, F, and G
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批准号:7681224
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项目类别:
-
资助金额:$74.6万
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财政年份:2007
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负责人:James D. Marks
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依托单位:
Development of botulinum neurotoxin immunotherapy, serotypes C, D, F, and G
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批准号:7919484
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项目类别:
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资助金额:$74.13万
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财政年份:2007
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负责人:James D. Marks
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依托单位:
Development of botulinum neurotoxin immunotherapy, serotypes C, D, F, and G
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批准号:7324328
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项目类别:
-
资助金额:$72.97万
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财政年份:2007
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负责人:James D. Marks
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依托单位:
Development of botulinum neurotoxin immunotherapy, serotypes C, D, F, and G
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批准号:7481114
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项目类别:
-
资助金额:$74.27万
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财政年份:2007
-
负责人:James D. Marks
-
依托单位:
Development of botulinum neurotoxin immunotherapy, serotypes C, D, F, and G
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批准号:8128718
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项目类别:
-
资助金额:$73.96万
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财政年份:2007
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负责人:James D. Marks
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依托单位:
海外基金