USING MASS SPEC TO DETERMINE MOLECULAR IDENTITY OF TUMOR SPECIFIC ANTIGEN

使用质谱确定肿瘤特异性抗原的分子身份

• 批准号: 8169743
• 负责人: James D. Marks
• 金额: $ 0.18万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-12 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169743
• 关键词: Antibodies; Antigen Targeting; Bacteriophages; Binding; Cancer Biology; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Immunoprecipitation; Immunotherapeutic agent; Institution; Lead; Malignant neoplasm of prostate; Methods; Molecular; Phage Display; Research; Research Personnel; Resources; Source; Tumor Antigens; United States National Institutes of Health; cancer cell; human monoclonal antibodies; novel; protein aminoacid sequence; tandem mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Antibody phage display is a useful method of developing fully human monoclonal antibodies against both known and novel tumor antigens. We have obtained over 70 phage antibodies that specifically bind to and internalize within prostate cancer cells. Uncovering their molecular identities will lead to better understanding of cancer biology and better assessment of these molecules as targets for targeted immunotherapeutics. We propose to use immunoprecipitation and tandem mass spectrometry to obtain peptide sequences of antigens targeted by the antibodies.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 抗体噬菌体展示是开发针对已知和新型肿瘤抗原的全人单克隆抗体的有用方法。我们已经获得了 70 多种噬菌体抗体，它们特异性结合并内化前列腺癌细胞。揭示它们的分子身份将有助于更好地了解癌症生物学，并更好地评估这些分子作为靶向免疫治疗的靶标。我们建议使用免疫沉淀和串联质谱法来获得抗体靶向的抗原的肽序列。