USING MASS SPEC TO DETERMINE MOLECULAR IDENTITY OF TUMOR SPECIFIC ANTIGEN

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 抗体噬菌体展示是制备针对已知和新的肿瘤抗原的全人单抗的有效方法。我们已经获得了70多个能与前列腺癌细胞结合并内化的噬菌体抗体。揭示它们的分子身份将有助于更好地理解癌症生物学，并更好地评估这些分子作为靶向免疫疗法的靶点。我们建议使用免疫沉淀和串联质谱学来获得抗体靶向的抗原的多肽序列。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Antibody phage display is a useful method of developing fully human monoclonal antibodies against both known and novel tumor antigens. We have obtained over 70 phage antibodies that specifically bind to and internalize within prostate cancer cells. Uncovering their molecular identities will lead to better understanding of cancer biology and better assessment of these molecules as targets for targeted immunotherapeutics. We propose to use immunoprecipitation and tandem mass spectrometry to obtain peptide sequences of antigens targeted by the antibodies.